Isolamento, identificação e avaliação da atividade antileucêmica de alcaloides indólicos monoterpênicos de Tabernaemontana salzmannii A. DC., Apocynaceae

Figueiredo, Elaine Rodrigues; Vieira, Ivo José Curcino; Souza, Jucimar Jorgeane de; Braz‐Filho, Raimundo; Mathias, Lêda; Kanashiro, Milton; Côrtes, Fernanda H.

doi:10.1590/s0102-695x2010000100016

Cited by 5 publications

(5 citation statements)

References 7 publications

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“…Coronaridine is characterized by the presence of a functional ether group in the aromatic ring, whereas voacangine and heyneanine present no substitution in the aromatic ring. The presence of this ether group in the aromatic ring may explain the greater cytotoxic activity of coronaridine against Hep-2 cells (Figueiredo et al , 2010). Coronaridine was also cytotoxic to the normal 3T3 cell line, demonstrating that this activity is not specific for tumor cells.…”

Section: Discussionmentioning

confidence: 99%

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Cytotoxicity and genotoxicity of coronaridine from Tabernaemontana catharinensis A.DC in a human laryngeal epithelial carcinoma cell line (Hep-2)

et al. 2013

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Cancer has become a major public health problem worldwide and the number of deaths due to this disease is increasing almost exponentially. In the constant search for new treatments, natural products of plant origin have provided a variety of new compounds to be explored as antitumor agents. Tabernaemontana catharinensis is a medicinal plant that produces alkaloids with expressive antitumor activity, such as heyneanine, coronaridine and voacangine. The aim of present study was firstly to screen the cytotoxic activity of the indole alkaloids heyneanine, coronaridine and voacangine against HeLa (human cervix tumor), 3T3 (normal mouse embryo fibroblasts), Hep-2 (human laryngeal epithelial carcinoma) and B-16 (murine skin) cell lines by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide); and secondly to analyze the apoptotic activity, cell membrane damage and genotoxicity of the compound that showed the best cytotoxic activity against the tumor cell lines tested. Coronaridine was the one that exhibited greater cytotoxic activity in the laryngeal carcinoma cell line Hep-2 (IC50 = 54.47 μg/mL) than the other alkaloids tested (voacangine IC50 = 159.33 g/mL, and heyneanine IC50 = 689.45 μg/mL). Coronaridine induced apoptosis in cell lines 3T3 and Hep-2, even at high concentrations. The evaluation of genotoxicity by comet assay showed further that coronaridine caused minimal DNA damage in the Hep-2 tumor cell line, and the LDH test showed that it did not affect the plasma membrane. These results suggest that further investigation of coronaridine as an antitumor agent has merit.

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Section: Discussionmentioning

confidence: 99%

“…Figueiredo et al (2010) tested the alkaloids coronaridine, (19S)-heyneanine, isovoacangine, isovoacristine and voacangine isolated from Tabernaemontana salzmannii and observed satisfactory cytotoxic activity against human leukemia cells (THP-1). Cell death due to apoptosis was detected in 100% of cells.…”

Section: Discussionmentioning

confidence: 99%

Cytotoxicity and genotoxicity of coronaridine from Tabernaemontana catharinensis A.DC in a human laryngeal epithelial carcinoma cell line (Hep-2)

et al. 2013

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“…A voacangina apresentou atividade como inibidora da angiogênese e agente antineoplásico, segundo Kim et al [29] , o composto inibiu a formação de novos vasos sanguíneos a partir dos pré-existentes na membrana corioalantóide em doses não tóxicas de forma dose-dependente; por promover a diminuição dos níveis de expressão do fator 1α induzível de hipóxia e gene alvo do fator de crescimento endotelial vascular (VEGF), suprimindo in vitro a formação tubular e quimio-invasão induzida por este fator. O alcaloide mostrou-se capaz de induzir morte celular por apoptose em células leucêmicas humanas THP-1 quando comparadas ao controle sulfato de vincristina (um bloqueador da progressão mitótica das células) [30] .…”

Section: Voacanginaunclassified

Etnobotânica da planta Tabernaemontana sananho Ruiz & Pavon (Apocynaceae) - Revisão Integrativa

2023

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A Tabernaemontana sananho Ruiz & Pavon (Apocynaceae) é uma planta usada como medicina pelos povos da América Latina com diferentes contextos antropológicos e botânicos. Popularmente conhecida no Brasil como “colírio da floresta” ou “sananga”, tem seu uso expandido das aldeias para centros urbanos. Esse estudo teve por objetivo detalhar a planta sob a perspectiva da medicina indígena e medicina tradicional ocidental, compreendendo os aspectos etnobotânicos, farmacognósicos e farmacológicos. Trata-se de uma revisão de literatura integrativa e qualitativa, com descritor Tabernaemontana sananho, nas línguas: português, espanhol e inglês, nas bases de dados PUBMED, LILACS e Portal de Periódicos da CAPES. Convergências plausíveis entre atribuições da planta para determinadas situações foram encontradas no sentido nociceptivo, anti-inflamatório, em parte antimicrobiano e no estado de vigília. Algumas propriedades descritas relacionam-se aos alcaloides indólicos monoterpenoides. Os achados sugerem necessidade de mais pesquisas com o extrato da planta quanto a averiguação de propriedades e uso racional da sananga por conter alcaloide de caráter irritante.

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“…Senyawa alkaloid monomer dari tumbuhan V. foetida seperti voacangin memiliki aktivitas sitotoksik kuat pada sel kanker HEPG-2, A375, NDA-MB-231, SHSY-5Y, dan CT26 [24], senyawa voacangin juga bekerja dengan cara memediasi apoptosis pada Tsel HP-1 dan HUVEC [25]. Menurut Bailly (2020), mekanisme kerja terpenoid sebagai antikanker adalah menghambat siklus sel, menghambat proliferasi sel, menginduksi apoptosis, menghalangi transisi sel epitel ke sel mesenkim, penghambatan migrasi dan invasi sel kanker, dan memodulasi sistem kekebalan PD-L1 [26].…”

Section: Hasil Dan Diskusiunclassified

In Vitro Cytotoxic Activity Test of Tampa Badak (Voacanga foetida (Blume) Leaves on L1210 Leukemia

Susanty¹

2021

J Sains Farm Klin

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Based on previous research, the ethanolic extract of the Voacanga foetida (Blume) Rolfe plant has cytotoxic activity using the Brine Shrimps Lethality test. Therefore, further tested the anticancer activity of extracts, fractions and isolated compounds from V. foetida leaves in vitro on L1210 leukemia cells using the direct cell count method with positive control doxorubicin. The results showed that the methanol extract, fraction of butanol fraction, ethyl acetate, and hexane, as well as isolated compounds Tb1 from fraction of hexane, Tb2 from fraction of ethyl acetate, and Tb3 from fraction of butanol leaves from V. foetida, had IC50 values of 4,4; 9; 8,4; 16,8; 0,9; 1,8; and 1,1 µg/mL, while doxorubicin has an IC 50 value of 0.2 µg/mL. Based on the IC 50 value, it showed that the extract, fraction, and isolated compound from Voacanga foetida leave had a strong cytotoxic effect as evidenced by the IC50 value < 20 µg/mL (extract and fraction) and < 4 µg/mL (isolate) for each test concentration.

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Isolamento, identificação e avaliação da atividade antileucêmica de alcaloides indólicos monoterpênicos de Tabernaemontana salzmannii A. DC., Apocynaceae

Cited by 5 publications

References 7 publications

Cytotoxicity and genotoxicity of coronaridine from Tabernaemontana catharinensis A.DC in a human laryngeal epithelial carcinoma cell line (Hep-2)

Cytotoxicity and genotoxicity of coronaridine from Tabernaemontana catharinensis A.DC in a human laryngeal epithelial carcinoma cell line (Hep-2)

Etnobotânica da planta Tabernaemontana sananho Ruiz & Pavon (Apocynaceae) - Revisão Integrativa

In Vitro Cytotoxic Activity Test of Tampa Badak (Voacanga foetida (Blume) Leaves on L1210 Leukemia

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