Cytotoxicity and genotoxicity of coronaridine from Tabernaemontana catharinensis A.DC in a human laryngeal epithelial carcinoma cell line (Hep-2)

Abstract

Cancer has become a major public health problem worldwide and the number of deaths due to this disease is increasing almost exponentially. In the constant search for new treatments, natural products of plant origin have provided a variety of new compounds to be explored as antitumor agents. Tabernaemontana catharinensis is a medicinal plant that produces alkaloids with expressive antitumor activity, such as heyneanine, coronaridine and voacangine. The aim of present study was firstly to screen the cytotoxic activity of the indole alkaloids heyneanine, coronaridine and voacangine against HeLa (human cervix tumor), 3T3 (normal mouse embryo fibroblasts), Hep-2 (human laryngeal epithelial carcinoma) and B-16 (murine skin) cell lines by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide); and secondly to analyze the apoptotic activity, cell membrane damage and genotoxicity of the compound that showed the best cytotoxic activity against the tumor cell lines tested. Coronaridine was the one that exhibited greater cytotoxic activity in the laryngeal carcinoma cell line Hep-2 (IC50 = 54.47 μg/mL) than the other alkaloids tested (voacangine IC50 = 159.33 g/mL, and heyneanine IC50 = 689.45 μg/mL). Coronaridine induced apoptosis in cell lines 3T3 and Hep-2, even at high concentrations. The evaluation of genotoxicity by comet assay showed further that coronaridine caused minimal DNA damage in the Hep-2 tumor cell line, and the LDH test showed that it did not affect the plasma membrane. These results suggest that further investigation of coronaridine as an antitumor agent has merit.

Antiviral effect of compounds derived from the seeds of Mammea americana and Tabernaemontana cymosa on Dengue and Chikungunya virus infections

“…2b). For the coumarins, it has been reported that voacangine inhibits the proliferation of endothelial tumor cells [58], and in non-tumor cells, a CC 50 greater than 400 μg/mL has been reported, which is consistent with our results [59]. In the case of lupeol acetate, although there are no reports on its anti-proliferative activity, it has been demonstrated that the CC 50 of lupeol (a structurally related compound) in VERO cells is greater than 300 μg/mL, which is also consistent with our results [60].…”

Chemical Characterization of Enteromorpha prolifera Extract Obtained by Enzyme-Assisted Extraction and Its Influence on the Metabolic Activity of Caco-2

“…Algal extracts obtained using the EAE method were also not toxic toward other epithelial cell lines, e.g., VERO [11,44]. Typically, the extracts prepared using standard methods, e.g., organic or water, are cytotoxic at a very low concentration of algal biomass and are considered as potential anticancer agents [45,46]. In turn, the algal extracts obtained using the EAE method are well-tolerated by mammalian cells even at high concentrations [11,44].…”

Gene Expression Response ofTrichophyton rubrumduring Coculture on Keratinocytes Exposed to Antifungal Agents

“…The cells were trypsinized (0.15% trypsin and 0.02% EDTA), counted in a hemocytometer (2.5 × 10 5 cells/well), and incubated in a 96-well plate for 24 h. A stock solution (10 mg/mL) of the antifungal agents was prepared in 10% DMSO and each agent was directly diluted in RPMI medium. After addition of the antifungals in fresh medium, the cells were cultured at 37°C in a 5% CO 2 atmosphere for 24 h and cytotoxicity was analyzed by the MTT assay [ 11 ], with modifications, as described by Rizo et al, 2013 [ 12 ]. Briefly, 20 μ L MTT/well (5 mg/mL in Hanks solution) was added to the 96-well plate and incubated for 4 h under the same conditions.…”