Determinação espectrométrica dos flavonóides das folhas de Maytenus (Celastraceae) e de Passiflora (Passifloraceae) e comparação com método CLAE-UV

Abstract

RESUMO:Este trabalho apresenta uma modificação dos procedimentos descritos nas Farmacopéias Francesa e Européia para a análise de flavonoides de Passiflora incarnata L., Passifloraceae, por espectrometria UV-Visível e propõe a sua aplicação na determinação dos flavonoides totais das folhas da espinheira-santa (Maytenus aquifolium Mart. e Maytenus ilicifolia (Schrad.) Planch., Celastraceae) e do maracujá (Passiflora edulis Sims. e Passiflora alata Curtis, Passifloraceae). Os resultados obtidos por espectrometria no UV-Visível foram comparados aos obtidos por cromatografia líquida de alta eficiência (CLAE-UV), encontrando-se resultados estatisticamente similares entre os métodos espectrométrico modificado da Farmacopéia Francesa e CLAE-UV.Unitermos: flavonoides, espectrometria UV-Visível, fitoterápicos, Maytenus spp., Passiflora spp.ABSTRACT: "Spectrometric determination of flavonoids from Maytenus (Celastraceae) and Passiflora (Passifloraceae) leaves and comparison with an HPLC-UV method". This paper reports on a modification of the spectrometric procedures originally described in the French and European Pharmacopoeia for the analysis of Passiflora incarnata L. (Passifloraceae) flavonoids, proposing its application in the determination of total flavonoids from "espinheira-santa" (Maytenus aquifolium Mart. and Maytenus ilicifolia (Schrad.) Planch., Celastraceae) and "maracujá" leaves (Passiflora edulis Sims and Passiflora alata Curtis, Passifloraceae). A comparison was made of the results obtained by the spectrometric procedure with those obtained by high performance liquid chromatography (HPLC-UV), which demonstrated complete compatibility between the modified French Pharmacopoeia (spectrometric) and HPLC-UV methods.

Glycemic Regulation and Cytotoxic Characteristics of the Ethyl Acetate Fraction from <em>Passiflora cincinnata</em> Mast Leaves

“…Mast showed a high content of phenolic compounds in the form of tannins, flavonoids and flavonols in relation to other species of the genus [11,20,21,22,23], strengthening the evidence that flavonoids are the major components in several species of the genus Passiflora (table 1). To corroborate with these observations, it was previously shown that ExEt had a high amount of phenolic compounds and flavonoids using the HPLC analyzis [24].…”

Plants used to treat snakebites in Santarém, western Pará, Brazil: An assessment of their effectiveness in inhibiting hemorrhagic activity induced by Bothrops jararaca venom

“…Hydrated catechin (Sigma) was used for the standard curve (0.1 to 1.0 mg/mL, R=0.99), and absorbance was read at 500 nm. The test to determine the concentration of flavonoids was based on complexation of these compounds with AlCl 3 (Vetec), as described by Chabariberi et al (2009). Hydrated rutin (Sigma) was used for the standard curve (5 to 50 mg/mL, R= 0.99), and absorbance was read at 425 nm.…”

Applying design of experiments (DOE) to flavonoid extraction from Passiflora alata and P. edulis

“…The remaining aqueous extracted material was lyophilized in an Edwards lyophilizer (Brazil) at 0.9 mbar (-40 ºC). The total flavonoids content of each extract (5 and 2.5 mg/mL) compared to a compensation solution were measured spectrophotometrically in triplicate at 427 nm based upon the formation of a complex with 2% AlCl 3 (Pozzi, 2007;Chabariberi et al, 2009). …”

“…Pozzi (2007) and Chabariberi et al (2009) reported on a modification of the spectrometric procedures originally described in the French Pharmacopoeia for the analysis of Passiflora incarnata L., Passifloraceae, flavonoids and proposed its application in the determination of total flavonoids from P. edulis and P. alata leaves. In these studies, the results obtained by a spectrometric procedure were compared to those obtained by high performance liquid chromatography (HPLC-UV).…”

“…In these studies, the results obtained by a spectrometric procedure were compared to those obtained by high performance liquid chromatography (HPLC-UV). The authors demonstrated complete compatibility between the modified French Pharmacopoeia (spectrometric) and HPLC-UV methods (Pozzi, 2007;Chabariberi et al, 2009).…”