Assessment of effects of a formula used in the traditional Chinese medicine (Buzhong Yi Qi Wan) on the morphologic and osmotic fragility of red blood cells

Giani, Tania Santos; Paoli, Severo de; Presta, G.; Maiworm, Adalgisa Ieda; Santos-Filho, Sebastião David; Fonseca, A S; Bernardo-Filho, Mário

doi:10.1590/s0102-695x2007000400005

Cited by 2 publications

(2 citation statements)

References 27 publications

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“…The results obtained with the quality comparison of the shape of the RBC (non treated and treated with natural extracts) under optical microscopy could justify the modifications in the uptake of 99 mTc for the red blood cells in the presence of Mentha crispa extract, similar to that observed with the extract of Maytenus iliciofolia [ [18][19][20][21][22].…”

Section: Discussionmentioning

confidence: 55%

Cellular effects of an aqueous solution of Losartan® on the survival of Escherichia coli AB1157 in the presence and absence of SnCl2, and on the physiological property (osmotic fragility) of the erytrocyte

Zaidan¹,

Matos²,

Machado³

et al. 2010

ABB

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The angiotensin receptors type 1 (AT1) have affinity by Losartan®, low affinity to non-peptides antagonists and similar effect as Angiotensin-convert-enzyme inhibitors. It have been reported that natural and synthetic products might reduce the genotoxic and cytotoxic effects related to stannous chloride (SnCl2). SnCl2 is used in nuclear medicine as a reducing agent to obtain technetium-99 m-radiopharmaceuticals. The aim of this work was to evaluate the cellular effects produced by a solution of Losartan® (25 mg/ml) on the survival of Escherichia coli AB1157 in the presence and absence of SnCl2, and on the osmotic fragility of erythrocytes of the blood of Wistar rats. Briefly, blood sample was withdrawn by Wistar rats with heparinized syringe and incubated with Losartan® solution. Saline (NaCL 0.9%) was used as a control. The samples were gently mixed with hypotonic solutions of NaCl. After that it was centrifuged and the supernadant isolated for optical determination of the hemoglobin present. E. coli AB1157 cultures (exponential growth phase) were collected by centrifugation, washed and resuspended in 0.9% NaCl. Samples were incubated in water bath shaker with: (a) SnCl2 (25 μg/ml), (b) Losartan® (25 mg/ml) and (c) SnCl2 (25 μg/ml) + Losartan® (25 mg/ml). Incubation with 0.9% NaCl was also carried out (control). At 60 min intervals, aliquots were withdrawn, diluted, spread onto Petri dishes with solid LB medium and incubated overnight. The colonies formed were counted and the survival fractions calculated. Statistical analysis was performed. The results showed that there was a significantly increase (P < 0.05) in the osmotic fragility of the blood cells treated with Losartan®. Moreover, Losartan® was also able to protect the E. coli cultures against the lesive action of SnCl2. Although, in erythrocyte the osmotic fragility was increased by the presence of Losartan® that could 1) alter the physical properties of this cell, or 2) had a direct or indirect effect on the intracellular sodium concentration or 3) had acted on the cardiovascular system. It suggested that the Losartan® did interfere strongly with cellular metabolism and did alter the survival fractions of E. coli AB1157.

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Section: Discussionmentioning

confidence: 55%

Cellular effects of an aqueous solution of Losartan® on the survival of Escherichia coli AB1157 in the presence and absence of SnCl2, and on the physiological property (osmotic fragility) of the erytrocyte

Zaidan¹,

Matos²,

Machado³

et al. 2010

ABB

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“…The osmotic fragility assay is a classical, rapid, useful and easy technique that has permitted to obtain relevant information about the interactions of natural and synthetic drugs with cellular membrane (Khanna et al, 2002). Morphological analysis is another available method that has permitted to evaluate the effects of natural products on membrane of red blood cells (Oliveira et al, 2002, Oliveira et al, 2003Presta et al, 2007;Giani et al, 2007).…”

Section: Discussionmentioning

confidence: 99%

Osmotic and morphological effects on red blood cell membrane: action of an aqueous extract of Lantana camara

Maiworm¹,

Presta²,

Santos-Filho³

et al. 2008

Rev. bras. farmacogn.

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RESUMO: "Efeitos osmótico e morfológico na membrana de hemácias: ação de um extrato aquoso de Lantana camara". As infusões de folhas de Lantana camara (cambara-de-espinho) são usadas popularmente em alguns países para tratar doenças gastrointestinais. O experimento de fragilidade osmótica e a análise morfométrica têm sido usados para verifi car a interação de drogas com a membrana de hemácias. O objetivo deste trabalho foi avaliar os efeitos de um extrato aquoso de Lantana camara na fragilidade osmótica e na morfologia de hemácias. Amostras de sangue foram tratadas com extrato de Lantana camara (10 mg/mL), o ensaio de fragilidade osmótica e a análise morfológica foram realizadas. Na presença do extrato, os dados obtidos indicaram (i) um aumento signifi cativo (p < 0,05) da hemólise e (ii) modifi cações na morfologia das hemácias. Estes efeitos da Lantana camara poderiam estar associados com algumas propriedades farmacológicas de compostos químicos do extrato estudado.Unitermos: Lantana camara, Verbenaceae, morfologia, fragilidade osmótica, hemácias. ABSTRACT:The Lantana camara ("cambara de espinho") leaves infusions are used popularly in some countries to treat gastrointestinal diseases. Osmotic fragility assay and morphometric analysis have been used to verify the interaction of drugs with the membrane of red blood cells (RBC). The aim of this work was to evaluate the effects of an aqueous extract of Lantana camara on the osmotic fragility and on the morphology of RBC. Blood samples were treated with extract of Lantana camara (10 mg/mL), osmotic fragility assay and morphological analysis were carried out. In the presence of the extract, the data obtained indicated (i) a signifi cant (p < 0.05) increase of hemolysis and (ii) modifi cations on the morphology of RBC. These effects of the Lantana camara may be associated with some pharmacological properties of the chemical compounds of this studied extract.

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Assessment of effects of a formula used in the traditional Chinese medicine (Buzhong Yi Qi Wan) on the morphologic and osmotic fragility of red blood cells

Cited by 2 publications

References 27 publications

Cellular effects of an aqueous solution of Losartan® on the survival of Escherichia coli AB1157 in the presence and absence of SnCl2, and on the physiological property (osmotic fragility) of the erytrocyte

Cellular effects of an aqueous solution of Losartan® on the survival of Escherichia coli AB1157 in the presence and absence of SnCl2, and on the physiological property (osmotic fragility) of the erytrocyte

Osmotic and morphological effects on red blood cell membrane: action of an aqueous extract of Lantana camara

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Assessment of effects of a formula used in the traditional Chinese medicine (Buzhong Yi Qi Wan) on the morphologic and osmotic fragility of red blood cells

Cited by 2 publications

References 27 publications

Cellular effects of an aqueous solution of Losartan&#174; on the survival of Escherichia coli AB1157 in the presence and absence of SnCl2, and on the physiological property (osmotic fragility) of the erytrocyte

Cellular effects of an aqueous solution of Losartan&#174; on the survival of Escherichia coli AB1157 in the presence and absence of SnCl2, and on the physiological property (osmotic fragility) of the erytrocyte

Osmotic and morphological effects on red blood cell membrane: action of an aqueous extract of Lantana camara

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Cellular effects of an aqueous solution of Losartan® on the survival of Escherichia coli AB1157 in the presence and absence of SnCl2, and on the physiological property (osmotic fragility) of the erytrocyte

Cellular effects of an aqueous solution of Losartan® on the survival of Escherichia coli AB1157 in the presence and absence of SnCl2, and on the physiological property (osmotic fragility) of the erytrocyte