Avaliação in vitro das atividades antiinflamatória, antioxidante e antimicrobiana do alcalóide montanina

Abstract

RESUMO:Diversas propriedades biológicas, dentre as quais atividade antiparasitária, antitumoral, antiviral e anticolinesterásica são associadas aos extratos e produtos isolados, incluindo alcalóides de espécies de Amarilidáceas. Neste trabalho verifi cou-se para o alcalóide montanina, isolado dos bulbos de Rhodophiala bifi da (Herb.) Traub (Amaryllidaceae), atividade antioxidante e ação inibitória sobre o crescimento de culturas de Saccharomyces cerevisae (ATCC 2601), Pseudomonas aeruginosa (ATCC 27853), Staphylococcus aureus (ATCC 6538), Staphylococcus epidermides (ATCC 12228) e Escherichia coli (ATCC 25922). A atividade antiinfl amatória avaliada através da determinação da capacidade antiquimiotáxica da amostra apresentou resultados não signifi cativos na dose empregada.Unitermos: Amaryllidaceae, Rhodophiala bifi da, montanina, alcalóides.ABSTRACT: "In vitro evaluation of the antioxidant, anti-infl ammatory and antimicrobial activities of the montanine alkaloid". Several biological properties, such as antiparasite, antitumor, antiviral and anticholinesterasic activities, are associated with extracts and isolated products, including alkaloids from Amaryllidaceae species. In this study, the antioxidant activity and inhibitory action on the growth of cell cultures of Saccharomyces cerevisae (ATCC 2601), Pseudomonas aeruginosa (ATCC 27853), Staphylococcus aureus (ATCC 6538), Staphylococcus epidermides (ATCC 12228) and Escherichia coli (ATCC 25922) were observed to the montanine alkaloid, isolated from the bulbs of Rhodophiala bifi da (Herb.) Traub (Amaryllidaceae). The antiinfl ammatory activity, which was evaluated through the sample antichemotaxis ability determination, was not signifi cant in the used dose.

Antidiabetic and antiradical activities of plants from Venezuelan Amazon

“…Alkaloids were detected in almost all the extracts studied (Table 1). Those compounds are known to exhibit a variety of biological activities including inhibition of malignant cell growth and proliferation, leishmanicidal, anti-HIV, anti-infl ammatory and antioxidant activities (Bacher et al, 2001;Chan-Bacab and Peña-Rodríguez, 2001;Ra ková et al, 2004;Singh et al, 2005;Barbosa-Filho et al, 2006;Castilhos et al, 2007). On the other hand, secoiridoids are reported to show leishmanicidal and antioxidant activity (ChanBacab and Peña-Rodríguez, 2001;Carrasco-Pancorbo et al, 2005).…”

Antifungal constituents of the plant family Amaryllidaceae

“…Minimum quantity (μg) required for activity; na, not active. Standards: Nystatin MICs 4, 8, and 8 μg/ml against C. albicans , C. parapsilosis , and C. neoformans , respectively (Pagning et al, ); Chlorotrimazole MIC not given against C. albicans (Evidente et al, ); Amphotericin B MIC 0.6 μg/ml against C. albicans (Bonvicini et al, ); Tioconazole MICs 4, 0.03, 0.125, and 0.03 μg/ml against C. albicans , C. dubliniensis , C. glabrata , and L. elongisporus , respectively (Ločárek et al, ); Ketoconazole MIC not given against S. cerevisiae (Castilhos et al, ); Ketoconazole MICs 0.87, 0.92, and 1.26 μg/ml against A. niger , C. albicans , and T. rubrum , respectively (Wu et al, ).…”

“…In addition to phenanthridones, lycoranes, and crinanes, four other structural groups were represented in antifungal screenings of Amaryllidaceae alkaloids. These included members of the galanthamine ( 1 ), tazettine ( 24 ), homolycorine ( 25 ), and montanine ( 26 ) groups (Scheme ) (Castilhos et al, ; Evidente et al, ; Jimenez et al, ; Ločárek et al, ). As early as 1975, Jimenez et al showed that activities for pretazettine ( 27 ) against the S. cerevisiae strains Y166 and TR 1 were mild (MICs >2 mM; Table ).…”

“…Hippeastrine ( 28 ) was the only homolycorine representative on the list and has been screened against C. albicans in which its activity was moderate (MIC 125 μg/ml) (Evidente et al, ). The two montanine group members were exposed to S. cerevisiae and C. albicans cultures, respectively, where 10 μg of montanine ( 26 ) was the minimum quantity required for activity against the former, and a MIC of 188 μg/ml was determined for pancracine ( 29 ) in the latter pathogen (Castilhos et al, ; Evidente et al, ). Finally, the galanthamine group members, galanthamine ( 1 ), chlidanthine ( 30 ), and lycoramine ( 31 ), were all inactive against C. albicans and C. glabrata but had MICs of 512 μg/ml each against both C. dubliniensis and L. elongisporus (Ločárek et al, ).…”

The Brazilian Amaryllidaceae as a source of acetylcholinesterase inhibitory alkaloids

“…Galanthamine (purity [ 99 %) used for the calibration curves was previously obtained by the authors, and codeine (purity C 99 %) used as internal standard was purchased from Sigma Aldrich (St. Louis, MO, USA). (Castilhos et al 2007;da Silva 2005;da Silva et al 2008;Pagliosa et al 2010;Giordani et al 2011a, b;de Andrade et al 2011;Sebben 2005).…”