Schinus terebinthifolius raddi (anacardiaceae) no processo de cicatrização de gastrorrafias em ratos

Abstract

-Background -Gastrorraphy, isolated or associated with the use of biological adhesives, was throughout the history of surgery the usual way to promote healing in gastric lesions and the use of herbal medicine has been increasingly more employed. Aim -To evaluate the wound healing in the stomach of rats with the use of the hydroalcoholic extract of Schinus terebinthifolius Raddi (aroeira). Methods -Sixty rats, adult males, were divided into two groups: aroeira group and control group. Each one was subdivided into four subgroups of 15 animals (test groups). Each subdivided subgroup was also subdivided into three subgroups of five rats (deaths periods of 7, 14 and 21 days). All animals underwent the same surgical procedure (injury and stomach suture); animals in the aroeira group received daily dose of 100 mg/kg of hydroalcoholic extract via gavage while the control group received isotonic saline solution. Parameters evaluated were: macroscopic and microscopic changes, test for resistance to insufflation of atmospheric air and test for tensile strength. Results -All animals had good healing of the abdominal wall and gastrorraphies without infection and dehiscence. Both groups had adhesions to the gastrorraphies surfaces with neighboring organs. The resistance test by insufflation of atmospheric air and tensile strength showed higher average of pressure on the 7th day and breaking strength in the time periods for the aroeira group. The intensity of chronic inflammation revealed statistically significant differences in the variables fibroblast proliferation and collagen. Conclusion -The use of hydroalcoholic extract of Schinus terebinthifolius Raddi accelerated the stomach healing in rats ABCDDV/862 RESUMO -Racional -A gastrorrafia isolada ou associada ao uso de adesivos biológicos constituiu ao longo da história da cirurgia a forma usual de promover a cicatrização nas lesões gástricas e o uso de fitoterápicos tem sido cada vez mais empregado. Objetivo -Avaliar a cicatrização de ferida provocada no estômago de ratos com uso do extrato hidroalcoólico de Schinus terebinthifolius Raddi. Métodos -Foram utilizados 60 ratos, adultos, machos, distribuídos em dois grupos: grupo aroeira e grupo controle, os quais foram subdivididos em três subgrupos de cinco animais conforme o momento das mortes dos animais (7, 14 e 21 dias). Todos os animais foram submetidos ao mesmo procedimento cirúrgico (lesão e rafia do estômago) diferindo apenas que os animais do grupo aroeira receberam dose diária de 100 mg/kg do extrato hidroalcoólico via gavagem enquanto o grupo controle recebeu solução salina isotônica. Os parâmetros avaliados foram: alterações macroscópicas e microscópicas, teste de resistência à insuflação de ar atmosférico e teste pela força de tração. Resultados -Todos os animais demonstraram boa cicatrização da parede abdominal e das gastrorrafias, sem infecção e deiscência. Ambos os grupos apresentaram aderências à superfície das gastrorrafias com órgãos vizinhos. O teste de resistência por insuflação de ar atmosféric...

Schinus terebinthifolius raddi (Aroeira) and Orbignya phalerata mart. (Babassu) effect in cecorrahphy healing in rats

“…In relation to fibroblast proliferation, there was a significant difference at 14 days between the GC and GB, favoring GC and GA, similar to other studies 24,25 . Another important variable to assess the chronic inflammatory process is fibrosis or collagen deposition.…”

“…Another important variable to assess the chronic inflammatory process is fibrosis or collagen deposition. In this research it was observed to be missing/discrete in all groups without significant difference; other studies showed significant differences favoring the experimental group 26,27 and, in contrast, favoring the GC 24 . In the gastrointestinal tract, collagen is synthesized by fibroblasts and smooth muscle cells, predominantly in the scar collagen type I (68%) that plays an important role in the tensile strength of the scar 18 .…”

“…Studies of Lucena et al 9 and Santos et al 25 showed intensity on day 7 of chronic inflammation favoring the experimental group with a significant difference in angiogenesis variable. In the present study, there was significant difference on day 14 in GC and GA. Santos et al 24 found no difference between groups. Batista et al 29 have published significant trend for the experimental group (babassu), suggesting that longer observation could point to advantages of using herbal medicine.…”

“…Already the oral route of administration by daily gavage 24,25 differs from other papers 23,26 using it parenterally, intraperitoneally and even topically in different dosages, proving that this herbal can be used by different administration routes. Was considered by the authors of this study that daily administration would give better action opportunity and could contribute to the literature with alternative higher dosage.…”

Resistance to rupture of the equine stomach

“…An aneroid manometer was connected to the probe, and stomachs were immersed in water and injected with air at 0.1 mL/s. The release of bubbles in the water from a rupture indicated the occurrence of rupture, when pressure was measured in mm Hg (Santos et al 2012). …”