Comparação dos efeitos do laser de baixa potência e do ultrassom de baixa intensidade associado ao Biosilicato® no processo de reparo ósseo em tíbias de ratos

Oliveira, Poliani de; Fernandes, Kelly Rossetti; Sperandio, Evandro Fornias; Pastor, Fabio Alexandre Casarin; Nonaka, Keico Okino; Parizotto, Nivaldo Antônio; Rennó, Ana Cláudia Muniz

doi:10.1590/s0102-36162012000100014

Cited by 6 publications

(2 citation statements)

References 11 publications

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“…A similar result was found by Oliveira et al [30] when LIPUS equipment was used at an intensity of 30 mW/cm 2 . However, our findings are not yet conclusive regarding bone-healing acceleration.…”

Section: Discussionsupporting

confidence: 89%

The influence of low-intensity physiotherapeutic ultrasound on the initial stage of bone healing in rats: an experimental and simulation study

et al. 2016

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BackgroundLow-intensity physiotherapeutic ultrasound has been used in physical therapy clinics; however, there remain some scientific issues regarding the bone-healing process. The objective of this study was to investigate the influence of low-intensity physiotherapeutic ultrasound on the initial stage of bone healing in rats.MethodsTwenty-two male adult rats were assessed quantitatively and qualitatively using radiographic, biochemical, and histological analyses. Numerical simulations were also performed. Fractures in animals in the ultrasound group (n = 11) were treated with low-intensity ultrasound (pulsed mode, duty cycle 20 %) for 10 min daily at an intensity of 40 mW/cm2 SATA (1.0 MHz) for 10 days. Fractures in animals in the control group (n = 11) were not treated.ResultsAlkaline phosphatase levels were non-significantly higher in the ultrasound group than in the control group in the time intervals considered (t(13) = 0.440; 95 % confidence interval (CI) −13.79 to 20.82; p = 0.67). Between-group serum calcium levels were also not significantly different (t(13) = −0.842; 95 % CI −0.48 to 0.21; p = 0.42). Finally, there were no significant differences in radiological scores between the two groups (U = 118; 95 % CI −1.99 to 1.99; p = 0.72). However, the diameter of the newly formed bone tissue was greater and more evident in the ultrasound group.ConclusionsThirteen days after fracture, there was no significant between-group differences in bone-healing processes, although the increased alkaline phosphatase levels and diameter of new bone tissue need to be further investigated.

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Section: Discussionsupporting

confidence: 89%

The influence of low-intensity physiotherapeutic ultrasound on the initial stage of bone healing in rats: an experimental and simulation study

et al. 2016

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“…Atualmente, o laser de baixa potência tem ganhado grande destaque mundial nas Ciências da Saúde em função de busca por formas menos invasivas de tratamento. Com isso, tornouse um método muito utilizado na maioria dos países e é estudada mundialmente, e a sua popularidade está relacionada não só a sua ação menos invasiva, mas também por ser atérmica, indolor, baixo custo (DE OLIVEIRA et al, 2012) e com tempo de aplicabilidade inferior aos demais recursos fisioterapêuticos (LINS et al, 2010). Grande variedade de condições patológicas utilizando a FBM é descrita na literatura, tais como: a modulação de processo inflamatório (CAETANO; ZANUTO, 2013), aceleração do reparo tecidual (LORETI, et al, 2015), alívio de dor e tratamento de algumas desordens neurológicas (STEIN et al, 2018).…”

Section: Fotobiomodulaçãounclassified

Efeitos da Fotobiomodulação em Células Tumorais in vitro: Revisão da Literatura

et al. 2021

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Atualmente, novos métodos de tratamento contra o câncer estão sendo investigados para a obtenção de um bom prognóstico e menor índice de efeitos colaterais, e uma das terapias atuais sob investigação é a Fotobiomodulação (FBM). Sabe-se que a proliferação de células normais e tumorais pode ser estimulada ou inibida pela FBM, e esse processo vai depender dos parâmetros utilizados. O objetivo deste estudo foi conduzir uma revisão da literatura, a fim de avaliar os efeitos biológicos de diferentes parâmetros da FBM sobre cultura de células tumorais (osteossarcoma). Realizou-se busca na literatura através das bases de dados PubMed/MEDLINE, Cochrane Library e EMBASE. Os estudos mostraram que a FBM pode ser utilizada em células cancerígenas de forma a diminuir a sua proliferação, contudo, a falta de padronização dos protocolos de irradiação a laser para investigações in vitro não permite estabelecer os parâmetros ideais para esse fim. Com base nos achados relatados se pode sugerir que a FBM, em parâmetros específicos, pode promover a bioestimulação ou até mesmo a inibição das células cancerígenas, sendo necessários mais estudos para determinar os protocolos de tratamento ideais e, por isso, deve ser usada com cautela na prática clínica. Palavras-chave: Terapia com Luz de Baixa Intensidade. Terapia a Laser. Osteossarcoma. Abstract Currently new treatment methods against cancer have been investigated to obtain a good prognosis and a lower rate of side effects, and one of the current therapies under investigation is Photobiomodulation (PBM). It is known that the normal and tumor cells proliferation can be stimulated or inhibited by PBM, and this process will depend on the parameters used. The objective of this study was to conduct a literature review in order to assess the biological effects of different PBM parameters on tumor cell culture (osteosarcoma). Literature search was performed through the PubMed/MEDLINE, Cochrane Library and EMBASE databases. Inclusion criteria were: to be an in vitro study with cancer cells, and to have at least one treatment group with PBM and a control group (without treatment). Studies have shown that PBM can be used in cancerous lesions in order to decrease the proliferation of these cells, however, the lack of standardization of laser irradiation protocols for in vitro investigations does not allow to establish the ideal parameters for this purpose. Based on the reported findings, it can be suggested that PBM in specific parameters may promote biostimulation or even inhibition of cancer cells. More studies are necessary to determine the ideal treatment protocols, and, because of this, it should be used with caution in clinical practice. Keywords: Low Intensity Light Therapy. Laser therapy. Osteosarcoma

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Evaluation of bone repair after application of a norbixin membrane scaffold with and without laser photobiomodulation (λ 780 nm)

et al. 2018

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Biocompatible membranes are widely used in medicine to stimulate bone repair. Several studies have demonstrated that laser photobiomodulation (PBM) also stimulates osteoblast proliferation and osteogenesis at the fracture site, leading to a greater deposition of bone mass and accelerating the process of bone consolidation. This work assessed the therapeutic effect of 780-nm laser PBM and a polystyrene membrane coated with norbixin and collagen (PSNC) on bone healing in rats with calvarial bone defect. Histological staining, Raman spectroscopy, and scanning electron microscopy (SEM) were used to evaluate the bone repair process. Four experimental treatment groups were compared: C, control; M, membrane only; L, laser PBM only; and ML, membrane + laser PBM. A bone defect was created in the calvaria of each animal, with each group subdivided into two subgroups that underwent euthanasia after 15 and 30 days treatment. The L and ML groups were irradiated (λ = 780 nm, ED = 6 J/cm, P = 60 mW, t = 4 s) postoperatively on alternate days until they were euthanized. The bone concentration of hydroxyapatite (CHA) showed a clear gradation with increasing phosphate area in the order B (normal cortical bone) > L > M > ML > C for both periods. The PSNC membrane was effective in reducing the inflammatory process and served as a scaffold for bone repair. The laser PBM also showed positive effects on the bone repair process with increased deposition and organization of the newly formed bone. However, laser PBM failed to improve the bioactive properties of the membrane scaffold.

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Comparação dos efeitos do laser de baixa potência e do ultrassom de baixa intensidade associado ao Biosilicato® no processo de reparo ósseo em tíbias de ratos

Cited by 6 publications

References 11 publications

The influence of low-intensity physiotherapeutic ultrasound on the initial stage of bone healing in rats: an experimental and simulation study

The influence of low-intensity physiotherapeutic ultrasound on the initial stage of bone healing in rats: an experimental and simulation study

Efeitos da Fotobiomodulação em Células Tumorais in vitro: Revisão da Literatura

Evaluation of bone repair after application of a norbixin membrane scaffold with and without laser photobiomodulation (λ 780 nm)

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