Isolation and molecular characterization of Rhizoctonia-like fungi associated with orchid roots in the Quadrilátero Ferrífero and Zona da Mata regions of the state of Minas Gerais, Brazil

Nogueira, Ricardo Eustáquio; Berg, Cássio van den; Pereira, Olinto Liparini; Kasuya, Maria Catarina Megumi

doi:10.1590/s0102-33062014000200017

Cited by 12 publications

(8 citation statements)

References 16 publications

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“…In Ecuador, Suarez et al (2006) isolated mycorrhizal fungi belonging to the genera Tulasnella and Sebacina. In Brazil, many clades of Tulasnella (Epulorhiza) have been found in several species (Pereira et al 2005;Nogueira et al 2014; and Ceratobasidium has also been detected in a recent study of Coppensia doniana (sin. Oncidium donianum) .…”

Section: Introductionmentioning

confidence: 87%

The epiphytic orchids Ionopsis utricularioides and Psygmorchis pusilla associate with different Ceratobasidium lineages at Valle del Cauca, Colombia

et al. 2015

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In Orchidaceae, association with symbiotic fungi is required for seed germination and seedling development, thereby being the main energy source during the first steps of germination. Colombia is one of the countries with the greatest biodiversity of orchids, with an estimated 3,200 species, but few studies on orchid mycorrhiza have been conducted. In our study, we isolated and sequenced the internal transcribed spacer rDNA region of fungi from two co-occurring Colombian epiphytic orchids, I. utricularioides and P. pusilla, both belonging to the subtribe Oncidiinae. All sequences were recognized as belonging to the genus Ceratobasidium, known to be a common orchid mycorrhizal fungus in both tropical and temperate orchids. One sequence was 100% similar to fungi isolated from I. utricularioides in Costa Rica in a previous study. I. utricularioides was confirmed to be a specialist, associating with only one clade of mycorrhizal fungi. However, P. pusilla was shown to be a generalist, associating with three clades. This finding indicates that the variation in mycorrhizal specificity could be an important factor in the co-existence of orchids. The high affinity between the subtribe Oncidiinae and Ceratobasidium was also reinforced.

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Section: Introductionmentioning

confidence: 87%

The epiphytic orchids Ionopsis utricularioides and Psygmorchis pusilla associate with different Ceratobasidium lineages at Valle del Cauca, Colombia

et al. 2015

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“…In Brazil, many isolated fungi from orchids belong to this respective anamorphic, the Epulorhiza genus, obtained from Epidendrum dendrobioides and Sophronitis milleri (Nogueira et al 2005), Epidendrum secundum (Pereira et al 2009;Nogueira et al 2014), Acianthera limae and Polystachya concreta (Nogueira et al 2014) and Cyrtopodium vernum (Gonçalves et al 2014).…”

Section: Isolation and Identification Of Mycorrhizal Fungusmentioning

confidence: 99%

Cyrtopodium paludicolum germination with two Tulasnella isolates

et al. 2017

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Symbiosis between orchid seeds and mycorrhizal fungi has been reported to be a determining factor in the success of germination and protocorm development in vitro. Th e aim of this study was to isolate and identify by molecular analysis the mycorrhizal fungus associated with Cyrtopodium paludicolum, and to evaluate its effi ciency in facilitating seed germination and development. Germination experiments were carried out using a fungus isolated from C. paludicolum (CH01) and Epidendrum secundum (M65), which has been successfully used a number of times in symbiotic germination. Th e experiments were conducted in a completely randomized design with treatments of CH01, M65 as well as under asymbiotic conditions. Th e mycobiont CH01 was successfully isolated from Cyrtopodium paludicolum and identifi ed as Tulasnella sp. Treatments with both fungi reached a higher germination percentage than under asymbiotic conditions, indicating no specifi city in the relationship between Cyrtopodium paludicolum and the fungi. Th e results presented have the potential to advance research into the propagation and conservation of C. paludicolum, a native of the Cerrado biome.

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“…The molecular and morphological analyses allowed us to identify the selected strains as different clades of Tulasnella, a genus included in the 'Rhizoctonia-like fungal complex' (Valadares & al. 2012;Nogueira & al. 2014;.…”

Section: Discussionmentioning

confidence: 99%

“…2004;Pereira & al. 2005;Nogueira & al., 2014), and it has also been documented in the Mexican photosynthetic orchids B. urbana Dressler and B. campanulata Lex. (Ortega-Larrocea & Rangel-Villafranco 2007).…”

Section: Discussionmentioning

confidence: 99%

Characterization of mycorrhizal fungi of the genus <em>Tulasnella (Tulasnellaceae, Basidiomycota)</em> in the genus of orchids <em>Bletia</em> from Barranca del Cupatitzio Natural Reserve, Mexico

Beltrán-Nambo

Montero-Castro

Martı́nez-Trujillo

et al. 2018

Anal. Jard. Bot. Madr.

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The goal of this study was the identification of mycorrhizal fungi associated with three terrestrial orchids of the genus Bletia Ruiz & Pav.: B. roezlii Rchb. f., B. purpurata A.Rich., and B. punctata Lex., in Barranca del Cupatitzio Natural Reserve - Michoacán, México - . Thirty-nine strains were isolated and morphologically characterized. Nine strains were selected from the molecular analysis. Bletia punctata, an endemic species of Mexico, showed the lowest variability in mycorrhizal fungi. Morphological analysis showed that 39 isolated strains belong to the ‘Rhizoctonia-like fungal complex’. According with the tree of Euclidian distances generated by the analysis WARD, all isolates were included into four subgroups, all related to the genus Tulasnella J.Schröt - which belongs to the ‘Rhizoctonia-like fungal complex’?. Molecular and phylogenetic analysis of the nine selected strains corroborated the results of the morphological study: the sequences obtained were clustered in four subclades related to species of Tulasnella. Our results indicate that a single species of Bletia from a single locality can be associated with different species of mycorrhizal fungi, at least during the adult stage and that the combination of morphological and molecular analyses is a good tool to identify orchid mycorrhizal fungi.

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Isolation and molecular characterization of Rhizoctonia-like fungi associated with orchid roots in the Quadrilátero Ferrífero and Zona da Mata regions of the state of Minas Gerais, Brazil

Cited by 12 publications

References 16 publications

The epiphytic orchids Ionopsis utricularioides and Psygmorchis pusilla associate with different Ceratobasidium lineages at Valle del Cauca, Colombia

The epiphytic orchids Ionopsis utricularioides and Psygmorchis pusilla associate with different Ceratobasidium lineages at Valle del Cauca, Colombia

Cyrtopodium paludicolum germination with two Tulasnella isolates

Characterization of mycorrhizal fungi of the genus <em>Tulasnella (Tulasnellaceae, Basidiomycota)</em> in the genus of orchids <em>Bletia</em> from Barranca del Cupatitzio Natural Reserve, Mexico

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