Uma nova espécie de Bacopa Aubl. (Scrophulariaceae) da América do Sul

Souza, Vinícius Castro

doi:10.1590/s0102-33062001000100007

Cited by 4 publications

(2 citation statements)

References 2 publications

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“…Morr., Angelonia integerrima Spreng. are currently considered as ancestral species [4] – [6] . We therefore use the term Angelonia × angustifolia .…”

Section: Introductionmentioning

confidence: 99%

Isolation of Dihydroflavonol 4-Reductase cDNA Clones from Angelonia x angustifolia and Heterologous Expression as GST Fusion Protein in Escherichia coli

et al. 2014

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Blue Angelonia × angustifolia flowers can show spontaneous mutations resulting in white/blue and white flower colourations. In such a white line, a loss of dihydroflavonol 4-reductase (DFR) activity was observed whereas chalcone synthase and flavanone 3-hydroxylase activity remained unchanged. Thus, cloning and characterization of a DFR of Angelonia flowers was carried out for the first time. Two full length DFR cDNA clones, Ang.DFR1 and Ang.DFR2, were obtained from a diploid chimeral white/blue Angelonia × angustifolia which demonstrated a 99% identity in their translated amino acid sequence. In comparison to Ang.DFR2, Ang.DFR1 was shown to contain an extra proline in a proline-rich region at the N-terminus along with two exchanges at the amino acids 12 and 26 in the translated amino acid sequence. The recombinant Ang.DFR2 obtained by heterologous expression in yeast was functionally active catalyzing the NADPH dependent reduction of dihydroquercetin (DHQ) and dihydromyricetin (DHM) to leucocyanidin and leucomyricetin, respectively. Dihydrokaempferol (DHK) in contrast was not accepted as a substrate despite the presence of asparagine in a position assumed to determine DHK acceptance. We show that substrate acceptance testing of DFRs provides biased results for DHM conversion if products are extracted with ethyl acetate. Recombinant Ang.DFR1 was inactive and functional activity could only be restored via exchanges of the amino acids in position 12 and 26 as well as the deletion of the extra proline. E. coli transformation of the pGEX-6P-1 vector harbouring the Ang.DFR2 and heterologous expression in E. coli resulted in functionally active enzymes before and after GST tag removal. Both the GST fusion protein and purified DFR minus the GST tag could be stored at −80°C for several months without loss of enzyme activity and demonstrated identical substrate specificity as the recombinant enzyme obtained from heterologous expression in yeast.

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“…Morr., Angelonia integerrima Spreng. are currently considered as ancestral species [4] – [6] . We therefore use the term Angelonia × angustifolia .…”

Section: Introductionmentioning

confidence: 99%

Isolation of Dihydroflavonol 4-Reductase cDNA Clones from Angelonia x angustifolia and Heterologous Expression as GST Fusion Protein in Escherichia coli

et al. 2014

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“…van Bruggen, 1968van Bruggen, , 1969van Bruggen, , 1970van Bruggen, , 1973Cook, 1974;McKinnon, 1997; 5.5 -6.0 -8.0 high D (heterosporous) Svenson, 1944;Moore, 1969;Cook, 1974;CTAHR, 2002;Beck, 2005;Aquafish.net, 2011;Invasive.org, 2011;. Barrett, 1978;Souza, 2001Souza, , 2009Tenenbaum, 2003;Aquascapingworld, n. Cook, 1974;Barrett, 1978;Hargreaves, 2006;APC, 2009;Gann et al ., 2011;. Cook, 1974;Barrett, 1978;Hargreaves, 2006;Efloras, 2008a;APC, 2009;Souza, 2009;Liveaquaria.com, 2011;.…”

Section: Only For Aquatic Plant Usersmentioning

confidence: 99%

Invasive aquatic plants and the aquarium and ornamental pond industries

Azan¹

2021

Preprint

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The purpose of the research was to clarify the common traits of a successful aquatic invasive plant. The biological traits of aquatic plants sold by aquarium and ornamental pond stores in the Greater Toronto Area, Ontario were analysed using logistic regression analyses (LRM). The multinomial LRM successfully predicts group identity 63% of the time, clearly differentiating native invasive and native non invasive from alien invasive (SI), but not ailen non invasive (ANI) from AI. In contrast, the binomial LRM effectively discriminates ANI from AI taxa 77% of the time. The variable that best discriminated between AI and ANI are type of propagation strategies, number of propagation strategies used, number of dispersal mechanisms used, minimum temperature, and sales volume of aquatic plants as a proxy for introduction. Recognizing the common traits of successful invaders allows for the identification of ANI taxa that have the potential to become invaders, prior to introduction, thus preventing future invasions.

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Notes on the Genus Bacopa (Plantaginaceae, Gratioleae) in the Orinoquia Region of Colombia and Venezuela

Aymard¹,

Rangel-Ch.

Minorta-Cely

et al. 2020

Harvard Papers in Botany

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Uma nova espécie de Bacopa Aubl. (Scrophulariaceae) da América do Sul

Abstract: Uma nova espécie de Bacopa (Scrophulariaceae) do Brasil e Argentina é descrita. Ilustração e descrição desta espécie são apresentadas, além de uma comparação com espécies afins do gênero.

Cited by 4 publications

References 2 publications

Isolation of Dihydroflavonol 4-Reductase cDNA Clones from Angelonia x angustifolia and Heterologous Expression as GST Fusion Protein in Escherichia coli

Isolation of Dihydroflavonol 4-Reductase cDNA Clones from Angelonia x angustifolia and Heterologous Expression as GST Fusion Protein in Escherichia coli

Invasive aquatic plants and the aquarium and ornamental pond industries

Notes on the Genus Bacopa (Plantaginaceae, Gratioleae) in the Orinoquia Region of Colombia and Venezuela

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