Comparação entre dois protocolos de tratamento de ceratoconjuntivite seca experimentalmente induzida em coelhos

Abstract

 RESUMOO objetivo deste estudo foi avaliar e comparar a eficácia de dois protocolos de tratamento de ceratoconjuntivite seca (CCS) experimentalmente induzida em coelhos: uma formulação oftálmica tópica composta por álcool polivinílico 1,4%, adicionado com acetilcisteína 10% e pilocarpina 1% (AAP), e outro protocolo com o uso do óleo de semente de linhaça (OL) tópico em forma de colírio, durante 12 semanas. Foram utilizados 15 coelhos machos, adultos, da raça Nova Zelândia, alocados aleatoriamente em três grupos: grupo C (controle), grupo AAP (formulação oftálmica) e grupo L (OL tópica). Os animais foram avaliados semanalmente pelo teste lacrimal de Schirmer, teste de fluoresceína e teste de Rosa Bengala; uma vez por mês, pelo exame de citologia esfoliativa ocular; ao final do experimento, pela análise histopatológica da córnea e conjuntiva. Os resultados demonstraram que houve um aumento maior na produção lacrimal quando utilizada a formulação oftálmica, e uma resolução mais rápida das úlceras de córnea, bem como diminuição no número de células desvitalizadas quando utilizado o óleo de semente de linhaça, além de aumento no número de células caliciformes em ambos os grupos de tratamento. A associação desses dois protocolos pode ser no futuro uma alternativa no tratamento da CCS.Palavras-chave: ceratoconjuntivite seca, álcool polivinílico 1,4%, acetilcisteína 10%, pilocarpina 1%, óleo de linhaça ABSTRACT The objective of this study was to evaluate and compare the effectiveness of two treatment protocol of experimentally induced keratoconjunctivitis sicca (KCS) in rabbits, a topical ophthalmic formulation composed by 1.4% povinilic alcohol added with 10% acetylcysteine and 1% pilocarpine (AAP) and another protocol with the topical use of the linseed seed oil (LO) in eye drop form for 12 weeks. Fifteen male New Zealand white rabbits were aleatory allocated in 3 groups: Group C (Control), Group AAP (ophthalmic formulation) and Group L (LO topical

Determination of intraocular pressure and Schirmer tear test and the comparison between the applanation tonometer (Tono‐Pen AVIA®) and the rebound tonometer (TonoVet Plus®) in mini lionhead rabbits

“…In our study, rabbits were cooperative and STT could be easily performed. Average tear production levels were 10.25 ± 3.75 mm/min in Mini Lionhead rabbits, similar to those found in New Zealand rabbits 13,31,32 . On the contrary, in the English Angora and Mini Holstein breeds, lower values of tear production were found, 5.4 ± 1.6 mm/min and 4.6 ± 1.2 mm/min, respectively 15 .…”

“…Average tear production levels were 10.25 ± 3.75 mm/min in Mini Lionhead rabbits, similar to those found in New Zealand rabbits. 13,31,32 On the contrary, in the English Angora and Mini Holstein breeds, lower values of tear production were found, 5.4 ± 1.6 mm/min and 4.6 ± 1.2 mm/min, respectively. 15 Possibly these small differences in tear production values are related to the breed distinction among the rabbits studied, which confirms the need to obtain breed ocular physiological parameters.…”

“…The determination of ophthalmic parameters, including IOP and tear production, are fundamental for the evaluation and monitoring of ocular diseases. The average values of IOP were measured with different tonometers, and the tear production was determined in different domestic and wild species, 9,[25][26][27][28][29] including New Zealand 6,12,13,15,16,18,21,[30][31][32][33] and Dutch Belted 34 rabbits. It is known that the physiological values of IOP and tear production vary considerably according to the methodology or tonometer used for measurement, as well as according to the species or even breed evaluated, 6,35 which makes the extrapolation of these parameters risky and imprecise.…”