Determinação da dose inseminante e embriogênese na fertilização artificial de tambaqui (Colossoma macropomum)

Leite, Liliane Veras; Melo, Mônica Aline Parente; Oliveira, F. C. E. de; Pinheiro, João Paulo Silva; Campello, C.C.; Nunes, J. F.; Salmito‐Vanderley, Carminda Sandra Brito

doi:10.1590/s0102-09352013000200018

Cited by 31 publications

(35 citation statements)

References 16 publications

(8 reference statements)

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“…The animals were weighed (4.10 ± 0.44 kg), measured (55.63 ± 2.33 cm) and then induced to spermiation via intracelomic with a single dose of carp pituitary extract (CPE; 2.5 mg kg -1 of the body weight) according to Leite et al (2013).…”

Section: Animal Handling Collection and Evaluation Of Semenmentioning

confidence: 99%

Cryopreservation of tambaqui semen using a dry shipper and a programmed freezing machine

Oliveira

Almeida‐Monteiro

Nunes

et al. 2016

SCA

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Tambaqui (Colossoma macropomum) is a native freshwater fish that is of great importance for Brazilian aquaculture. Because of this importance, several techniques have been developed to improve the reproduction of this species in captivity. One of these techniques is the cryopreservation of sperm. In an effort to increase the efficiency of cryopreservation protocols, researchers have tried to determine suitable diluting solutions and freezing methods, which will provide a better post-thaw sperm quality. Thus, this study aimed to evaluate the efficiency of different diluents and freezing methods for the cryopreservation of tambaqui (C. macropomum) sperm. Samples of fresh semen were diluted in different treatments (Glucose 5% + 10% Dimethyl sulfoxide -DMSO, Glucose 5% + 10% Methyl glycol -MG, BTS + 10% DMSO and BTS + 10% MG) at a 1:9 dilution rate and frozen in a programmed freezing machine and a dry shipper. The semen samples were thawed and evaluated for vitality, sperm morphology and kinetics. Cryopreserved semen with DMSO and using the programmed freezing machine provided a greater percentage of motile sperm (15.44 ± 1.04%) after thawing compared to the dry shipper (3.99 ± 0.55%), regardless of the diluent. Additionally, DMSO showed better sperm velocities than MG regardless of the freezing method and the extender employed. A higher percentage of living spermatozoa was observed when glucose (37.28 ± 1.32%) (regardless of the freezing method and cryoprotectant) and DMSO (37.98 ± 1.25%) was used in the programmed freezing machine. For morphology, a greater amount of normal spermatozoa (46.10 ± 1.82%) was observed when the semen was cryopreserved using a freezing machine programmed with DMSO as the cryoprotectant and Glucose or BTS (38.16 ± 1.9% and 39.26 ± 1.87%, respectively) as extenders. Therefore, we suggest the use of the DMSO (10%) cryoprotectant in association with the Glucose (5%) extended in the programmed freezing machine for cryopreservation of C. macropomum semen. ResumoO tambaqui (Colossoma macropomum) é uma espécie nativa de peixe de água doce de grande importância para aquicultura brasileira. Devido a isso, diversas técnicas têm sido desenvolvidas para aperfeiçoar a reprodução desta espécie em cativeiro, dentre elas a criopreservação de sêmen de peixe. Como uma forma de melhorar os protocolos de criopreservação, tem-se buscado utilizar soluções diluidoras e métodos de congelação adequados, proporcionando uma boa qualidade seminal pós-descongelação. Dessa forma, este estudo objetivou avaliar a eficiência de diferentes diluidores e métodos de congelação na criopreservação do sêmen de tambaqui (C. macropomum). As amostras de sêmen fresco foram diluídas em diferentes tratamentos (Glicose 5% + 10% Dimetilsufóxido -DMSO; Glicose 5% + 10% Metil glicol -MG; Beltsville Thawing Solution -BTS + 10% DMSO e BTS + 10% MG) na proporção 1:9 e congeladas em máquina de congelação programada e em Dry shipper. As amostras seminais foram descongeladas e avaliadas para vitalidade, morfologia e cinética espermát...

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Section: Animal Handling Collection and Evaluation Of Semenmentioning

confidence: 99%

Cryopreservation of tambaqui semen using a dry shipper and a programmed freezing machine

Oliveira

Almeida‐Monteiro

Nunes

et al. 2016

SCA

View full text Add to dashboard Cite

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“…Value equals to 1 indicates the perfect ellipse. The rugosity characterizes the amorphous cells, also being assigned values in scale from −1 to +1.This parameter can be positively correlated with head length, as well as being an indication of cell susceptibility to damage/rupture(Banaszewska, Andraszek, Czubaszek, & Biesiada-Drzazga, 2015;Leite, 2011;Sánchez, Bastir, & Roldan, 2013).…”

mentioning

confidence: 99%

Morphological and morphometric characterization of domestic cat epididymal sperm

Barbosa

Silva

Tabosa

et al. 2019

Reprod Domestic Animals

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Sperm morphometry is the tool that confers objectivity to the morphological evaluation by accurately measuring the dimensions of the gamete and its structures. Thus, the aim of the study was to perform a morphometric characterization of the domestic cat sperm. Therefore, sperm samples were collected from twenty pairs of epididymis in a TRIS extender at 37ºC. An aliquot of the sample was used to make a smear with Rose Bengal solution, and afterwards, the morphology and morphometry were analysed. In the morphology, were quantified the percentage of normal sperm cells, morphological changes of head, midpiece and tail. In morphometry, each normal sperm cell was measured for length, width, area and perimeter of head and midpiece, tail length and total length. The parameters ellipticity, elongation, regularity and rugosity were also determined. The percentage of normal sperm was 67.21%. Of the abnormalities, the curled/folded tail, followed by the curved midpiece, abnormal shaped head and detached head were the most quantified. The sperm head presented 5.56 ± 0.01 μm and 3.10 ± 0.01 μm of length and width, respectively. The head area was 16.94 ± 0.05 μm2, while the perimeter was 16.16 ± 0.03 μm. In the derived parameters, the values were as follows: ellipticity of 1.81 ± 0.00; elongation of 21.39 ± 0.12; regularity of 0.81 ± 0.00; and rugosity of 0.14 ± 0.00. The midpiece presented length and width of 7.96 ± 0.01 μm and 0.76 ± 0.01 μm, respectively. The mean length of the sperm tail was 45.12 ± 0.06 μm, and the total cell size was 58.67 ± 0.06 μm. Thus, it was concluded that the cat sperm is an elongated cell, with high rugosity and regularity. The spermatic tail represents more than ¾ of the total length of the cell and the midpiece exceeds the length of the head.

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“…To measure sperm concentration semen samples were fixed in 4% formol-citrate solution in the proportion of 1:4000 (LEITE et al, 2013) and 20 μL aliquots of this solution were deposited in a Neubauer chamber and analyzed under an optical microscope with a 40x objective lens.…”

Section: Methodsmentioning

confidence: 99%

Seasonal Variation in Seminal Quality in Brazilian Bocachico (Teleostei, Characiformes)

et al. 2018

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The Brazilian bocachico, Prochilodus brevis, is a rheophilic fish. Although there is evidence that this species shows reproductive seasonality in the wild, in captivity hormonal induction techniques allow semen sampling in different seasons. This study aimed to compare the kinetics, morphology and biochemical composition of the semen of Brazilian bocachico in captivity when hormonally induced to breed in the reproductive and non-reproductive seasons. During sampling spermiation was hormonally induced in breeders. The concentrations of total protein, glucose, fructose, triglyceride, calcium and chloride were evaluated with biochemical kits. The pH data (6.5 to 8.5) suggest semen requires alkaline conditions, as expected for freshwater fish. Seminal plasma contained more protein (1.51 ± 0.06 dL g-1), glucose (79.44 ± 1.88 mg dL-1) and triglycerides (61.59 ± 8.10 mg dL-1) in the non-reproductive than the reproductive season, but calcium ions (15.98 ± 1.02 mg dL-1) showed the opposite pattern. There was a significant seasonal difference in sperm morphology, with a higher percentage of normal sperm in the reproductive season. From these data it can be concluded that the physical, kinetic, morphological and biochemical characteristics of semen of captive Prochilodus brevis are influenced by reproductive season.

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Determinação da dose inseminante e embriogênese na fertilização artificial de tambaqui (Colossoma macropomum)

Cited by 31 publications

References 16 publications

Cryopreservation of tambaqui semen using a dry shipper and a programmed freezing machine

Cryopreservation of tambaqui semen using a dry shipper and a programmed freezing machine

Morphological and morphometric characterization of domestic cat epididymal sperm

Seasonal Variation in Seminal Quality in Brazilian Bocachico (Teleostei, Characiformes)

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