2019
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Abstract: Sperm morphometry is the tool that confers objectivity to the morphological evaluation by accurately measuring the dimensions of the gamete and its structures. Thus, the aim of the study was to perform a morphometric characterization of the domestic cat sperm. Therefore, sperm samples were collected from twenty pairs of epididymis in a TRIS extender at 37ºC. An aliquot of the sample was used to make a smear with Rose Bengal solution, and afterwards, the morphology and morphometry were analysed. In the morpholo… Show more

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Cited by 5 publications
(11 citation statements)
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References 41 publications
(58 reference statements)
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“…A total of 100 sperm were randomly counted under a microscope (1000x) and classified as normal or abnormal. Sperm abnormalities were grouped into changes in the head, midpiece, tail and acrosome (CBRA, 2013;Barbosa et al, 2019).…”
Section: Sperm Evaluationmentioning
confidence: 99%
See 2 more Smart Citations
“…A total of 100 sperm were randomly counted under a microscope (1000x) and classified as normal or abnormal. Sperm abnormalities were grouped into changes in the head, midpiece, tail and acrosome (CBRA, 2013;Barbosa et al, 2019).…”
Section: Sperm Evaluationmentioning
confidence: 99%
“…Measurements were performed using ImageJ software version 1.4.3.67. Measurements with ImageJ followed the recommendations of published works that used this software for sperm morphometric characterization (Tremori et al, 2014;Barbosa et al, 2019). Measurements were performed by manually clicking on the image, selecting the appropriate software tool for the width and length dimensions (line selection tools), and for area and perimeter (area selection tools).…”
Section: Sperm Evaluationmentioning
confidence: 99%
See 1 more Smart Citation
“…Fluid leaving the testis is reabsorbed in efferent duct and caput of the epididymis [7,17] Osmotic pressure within the epididymis increases caudally [17,18] Sperm plasma membrane is composed of large amount of phospholipids, cholesterol and glycoproteins [6,19] Cholesterol stiffens the sperm plasma membrane and increases its permeability [6,7] Glycoproteins coating the plasma membrane change in the same manner during epididymal transit [19] Percentage of spermatozoa with swollen acrosome and incomplete condensed chromatin decreases as sperm migrates distally along the epididymis [3,15] Presence of selective abnormal spermatozoa removal (phagocytosis) [7,17] Retained cytoplasmic droplets will be detached at or post-ejaculation…”
Section: Common Features Referencesmentioning
confidence: 99%
“…The complete testes with epididymis were placed in a sterile plastic container in 0.9% NaCl solution at 37℃ and transported to the laboratory for further analysis. The epididymis was isolated, and sperm was obtained by making an incision in the epididymal duct (de Sousa Barbosa et al, 2019). The sperm was flushed with a five mL saline solution.…”
Section: Sample Collectionmentioning
confidence: 99%