Potencial antiviral da quercetina sobre o parvovírus canino

Abstract

RESUMOAvaliou-se o efeito do flavonoide quercetina na replicação do parvovírus canino in vitro por meio do ensaio de determinação da atividade virucida (ensaio 1), ensaio de determinação da atividade sobre a célula (ensaio 2) e ensaio de tempo de adição das drogas em diferentes etapas do ciclo replicativo viral (ensaio 3). A quercetina apresentou significante atividade antiviral, com valores máximos de redução do título viral de 96,3% no ensaio 1, 90% no ensaio 2 e 90% no ensaio 3. Os efeitos mais expressivos ocorreram nas etapas de adsorção e penetração viral. Os resultados deste trabalho sugerem a importância da quercetina para a medicina veterinária.Palavras-chave: cão, parvovírus, quercetina, antiviral ABSTRACT The in vitro effect of the flavonoid quercetin against canine parvovirus was evaluated. The antiviral activity of quercetin was evaluated by determining the virucidal activity (assay 1), determining the activity on the cell (assay 2) and using the time of addition assay to test the inhibition of the viral replication cycle (assay 3).Quercetin showed a significant antiviral activity, with maximum viral titer reduction of 96.3% in assay 1, 90% in assay 2 and 90% in assay 3. The most expressive effects occurred in the stages of viral adsorption and penetration. The results show the importance of quercetin for veterinary medicine.

Cytotoxicity and antiviral activity evaluation of Cymbopogon spp hydroethanolic extracts

“…These negative results differ from those reported in previous studies with these plant species. Divergences may be related to several variables, such as (1) variations in the concentrations of secondary metabolites present in the plants, related to factors that may influence compound extractions (discussed above); (2) the chemical structure of these metabolites; (3) the fact that the phytochemical mechanism of action on the interaction capacity with the virus is different for each pathogen (Carvalho et al, 2013); and (4) the possibility of synergism or antagonism between extracted compounds (Cos et al, 2006;Kumar, Pandey, 2013). The aim of this study was not to investigate the constituents or their relationships that could be responsible for the observed effects, both in the cytotoxicity assays and in the antiviral activity trials.…”