Cytotoxicity and antiviral activity evaluation of Cymbopogon spp hydroethanolic extracts

Chiamenti, Lisandra; Silva, Francini Pereira da; Schallemberger, Karoline; Demoliner, Meriane; Rigotto, Caroline; Fleck, Juliane Deise

doi:10.1590/s2175-97902019000118063

Cited by 22 publications

(18 citation statements)

References 31 publications

(31 reference statements)

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“…The percentage of reduction in the number of plaques was calculated as: A (%) = 100 – [( B x 100) / C ], where A is the % in the number of plaques reduced, B is the average number of plaques for treatments and C is the viral control number of plaques [32]. Half-maximal inhibitory concentration (IC 50 ) with the respective 95% confidence intervals (95% CI), was calculated.…”

Section: Methodsmentioning

confidence: 99%

Preliminary analysis of a neotropical plant extract antiviral potential against Chikungunya and Mayaro viruses

Pires

Silva

Schallenberger

et al. 2021

Preprint

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Chikungunya and Mayaro fevers are viral infectious diseases, without vaccine or treatment, that causes fever and arthralgia. Establishing novel antiviral tools capable of preventing or treating Chikungunya virus (CHIKV), and Mayaro virus (MAYV) infections are needed. The use of plant-based compounds that affect the replication cycles of these viruses has been proposed as a promising strategy. Chiococca alba (L.) Hitchc. is a neotropical plant used by Yucatec Mayas traditional healers, mainly, as antipyretic and antirheumatic. To evaluate the potential of C. alba methanolic extracts against CHIKV and MAYV through preliminary analysis in vitro and in silico . The cytotoxicity profile of two C. alba roots methanolic extracts in Vero cells was performed by lysosomal viability using the neutral red assay, and the antiviral potential was determined by plaque assay. We further assessed, through in silico computational predictions, the possible interactions between the active site of the nsP2 proteases of these viruses with some secondary metabolites present in C. alba extracts, identified by High-Performance Liquid Chromatography (HPLC). Our partial phytochemical analysis revealed the presence of flavonoids, and phenolic acids in the C. alba extracts. Our in vitro assays showed that both C. alba extracts inhibited more than 70% of CHIKV and MAYV activities at 60 µg/mL concentration. Based on our in silico computational predictions, the flavonoids naringin and vitexin showed the greatest affinity energies with the CHIKV and MAYV nsP2 proteases, revealing the great potential of these compounds as viral inhibitors. The findings described here indicates that C. alba extracts, or their secondary metabolites, as a potential source of novel antiviral compounds.

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Section: Methodsmentioning

confidence: 99%

Preliminary analysis of a neotropical plant extract antiviral potential against Chikungunya and Mayaro viruses

Pires

Silva

Schallenberger

et al. 2021

Preprint

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“…Human mastadenovirus (HAdV) causes numerous ailments such as respiratory infection, gastroenteritis, hepatitis, meningoencephalitis, pneumonia, and multiple others. Lemongrass extracts also induced cytotoxicity in the human lung adenocarcinoma cell line and kidney cell lines of the monkey and rendered antiviral activity against HAdV Preprints (www.preprints.org) | NOT PEER-REVIEWED | Posted: 21 June 2021 doi:10.20944/preprints202106.0500.v1 [81]. In the recent pandemic upsurge, the efficacy of LEO was suggested against influenza and coronaviruses SARS-CoV-2 as well, enhancing the relevance and importance of lemongrass oil even more [36,82].…”

Section: Antiviral Activitymentioning

confidence: 99%

Lemongrass Essential Oil Components with Antimicrobial and Anticancer Activities

Mukarram¹,

Choudhary²,

Khan³

et al. 2021

Preprint

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The prominent cultivation of lemongrass relies on the pharmacological incentives of its essential oil. The lemongrass essential oil (LEO) has a significant amount of citral (mixture of geranial and neral), isoneral, isogeranial, geraniol, geranyl acetate, citronellal, citronellol, germacrene-D, and elemol in addition to numerous other bioactive compounds. These components confer various medicinal activities to LEO including antifungal, antibacterial, antiviral, anticancer, and antioxidant properties. These attributes are commercially exploited in pharmaceutical, cosmetics, and food preservations industries. Furthermore, the employment of LEO in the treatment of cancer opens a new vista in the field of therapeutics. Although different LEO components have shown promising anticancer activities in vitro, these effects have not been assessed yet in humans. Further studies on the anticancer mechanisms exerted by lemongrass components are required. The present review intends to provide a timely discussion on the relevance of lemongrass extracts in cancer and health treatment, and in food industry applications.

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“…Eucalyptus globulus is effective against H1N1 and HSV1 viruses (Brochot et al 2017, Cermelli et al 2008. Cymbopogon citratus showed antiviral activity against human mastadenovirus (Chiamenti et al 2019), herpes simplex types 1 and 2 (Almeida et al 2018), Newcastle disease virus in vivo (Abraham-Oyiguh et al 2019), dengue virus (Rosmalena et al 2019), and measles virus (Nurul et al 2006). Aframomum melegueta showed activity against atypical fowl pox virus (Oladunmoye et al 2020) and human respiratory syncytial virus (Chang et al 2013).…”

Section: Exceptmentioning

confidence: 99%

Exploring the indigenous knowledge systems to respond to coronavirus infection 2019 in Cameroon

Fokou

Fokouo²

2020

Ethnobot. Res. App.

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Background: Control of coronavirus infection 2019 outbreaks lack specific vaccine or drugs, highlighting the need for appropriate interventions. Indigenous knowledge has been a source of medicinal agents for thousands of years and could help to circumvent this threat. We sought to know the current indigenous knowledge used by people living in Yaounde and Douala, Cameroon to protect against covid-19.Methods: This survey was conducted with twohundred and seventy-seven participants. A semistructured questionnaire aiming at understanding of the practice and common interventions use by people to prevent covid-19 infection. Interviews were conducted by telephone and face to face interviews.Results: A total of thirty-nine recipes consisting in thirty-eight distinct ingredients were recorded. The recipe ingredients comprising twenty-nine medicinal plants and nine other alternatives were used for covid-19 prevention in the city of Yaounde and Douala, Cameroon. The twenty-nine medicinal plant species corresponded to twenty-eight genera, arranged in twenty plant families. The most cited plants in recipes were Citrus aurantiifolia Christm., Zingiber officinale Roscoe, and Allium sativum L.. The most cited formulation besides the basic protective measures consisting in recipe 6 [Allium sativum L., Citrus aurantiifolia (Christm.) Swingle.and Zingiber officinale Roscoe (RFC=Relative frequency of citation 15.16%)]. Leaves were the most commonly utilized plant part with a frequency of 45%. From the thirty-nine recipes recorded, majority were decoction (69.23%; 27/39) and were administered orally. Conclusions:Results show common knowledge devoid of scientific proof for most hints. Thus, their clinical application is pending reasonable scientific data on recipes and their standardization to support safety and efficacy in humans.

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Cytotoxicity and antiviral activity evaluation of Cymbopogon spp hydroethanolic extracts

Cited by 22 publications

References 31 publications

Preliminary analysis of a neotropical plant extract antiviral potential against Chikungunya and Mayaro viruses

Preliminary analysis of a neotropical plant extract antiviral potential against Chikungunya and Mayaro viruses

Lemongrass Essential Oil Components with Antimicrobial and Anticancer Activities

Exploring the indigenous knowledge systems to respond to coronavirus infection 2019 in Cameroon

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