Diagnosis of Brucella ovis infection by serology and PCR in urine samples from naturally infected rams in the state of Piauí

Costa, Érica Azevedo; Santana, Farley; Carvalho, Ciro José Sousa de; Moustacas, Valéria Spyridion; Silva, S.M.M.S.; Santos, Renato L.

doi:10.1590/s0102-09352012000300029

Cited by 15 publications

(14 citation statements)

References 9 publications

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“…Experimentally or naturally infected rams often eliminate B . ovis in the semen and urine, which is thought to be the most important form of transmission of the disease [ 24 , 27 , 43 ]. The immunization protocol with encapsulated B .…”

Section: Discussionmentioning

confidence: 99%

Encapsulated Brucella ovis Lacking a Putative ATP-Binding Cassette Transporter (ΔabcBA) Protects against Wild Type Brucella ovis in Rams

et al. 2015

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This study aimed to evaluate protection induced by the vaccine candidate B. ovis ΔabcBA against experimental challenge with wild type B. ovis in rams. Rams were subcutaneously immunized with B. ovis ΔabcBA encapsulated with sterile alginate or with the non encapsulated vaccine strain. Serum, urine, and semen samples were collected during two months after immunization. The rams were then challenged with wild type B. ovis (ATCC25840), and the results were compared to non immunized and experimentally challenged rams. Immunization, particularly with encapsulated B. ovis ΔabcBA, prevented infection, secretion of wild type B. ovis in the semen and urine, shedding of neutrophils in the semen, and the development of clinical changes, gross and microscopic lesions induced by the wild type B. ovis reference strain. Collectively, our data indicates that the B. ovis ΔabcBA strain is an exceptionally good vaccine strain for preventing brucellosis caused by B. ovis infection in rams.

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Section: Discussionmentioning

confidence: 99%

Encapsulated Brucella ovis Lacking a Putative ATP-Binding Cassette Transporter (ΔabcBA) Protects against Wild Type Brucella ovis in Rams

et al. 2015

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“…Interestingly, all PCR-positive animals in this study were also serologically positive by ELISA, while none of the AGID-positive animals were positive by any other diagnostic method. Poor agreement between AGID and PCR has been previously demonstrated under field conditions [ 15 ]. Furthermore, AGID has low sensitivity since experimentally infected rams that shed viable B. ovis in the urine and semen may be negative by AGID [ 6 , 13 ].…”

Section: Discussionmentioning

confidence: 99%

Serologic and molecular evidence of Brucella ovis infection in ovine and caprine flocks in the State of Minas Gerais, Brazil

et al. 2016

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BackgroundBrucella ovis infection is one of the leading causes of sub fertility and infertility in ovine, been characterized mainly by epididymitis, orchitis and testicular atrophy in rams. This study aimed to determine the frequency of B. ovis positivity in rams and goats flocks in the State of Minas Gerais, Brazil, by agarose gel immunodiffusion (AGID), ELISA, Rose Bengal, PCR and bacteriological isolation as diagnostic tools.FindingsSerum and urine samples were collected from properties with sheep or goat flocks, or from properties with mixed flock. Out of 50 sheep flocks, 6 % (3/50) were seropositive by AGID while 4 % (2/50) were positive by urine PCR for B. ovis. Out of five goat farms, 20 % (1/5) were seropositive for B. ovis by AGID. Mixed flock farms had 11.1 % (2/18) of positivity by AGID. By ELISA, 19.5 % (8/41) of sheep properties and 61.1 % (11/18) of the properties with mixed flocks were positive for B. ovis. No samples were positive in the test of Rose Bengal, ruling out exposure to smooth LPS Brucella species (particularly Brucella melitensis) and indicating that the positive in the ELISA was associated with Brucella spp. LPS rough (presumably B. ovis). No urine sample from sheep or goat was positive by bacteriological isolation.ConclusionsOur results demonstrate serologic or molecular evidence of B. ovis infection in several rams and billy goats from meso-regions of the State of Minas Gerais, Brazil. Also, this study report the indirect ELISA as an important tool for the diagnosis of B. ovis infection, as indirect ELISA in this study demonstrated to be the most sensitive diagnostic method adopted.

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“…Generally, definitive diagnosis of these infections is based on bacterial isolation, which can be difficult due to lack of suitable selective media for isolation of A. seminis and H. somni [5,10]. Importantly, standardized serological tests are widely available only for the diagnosis of B. ovis infection [19], although serology has well documented limitations in these cases [19,20]. Furthermore, contaminating bacteria present in semen, urine and preputial wash can overgrow these pathogens, which has more fastidious growing.…”

Section: Discussionmentioning

confidence: 99%

Species-specific multiplex PCR for the diagnosis of Brucella ovis, Actinobacillus seminis, and Histophilus somni infection in rams

et al. 2013

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BackgroundInfectious ovine epididymitis results in substantial economic losses worldwide due to reproductive failure and culling of breeders. The most common causative agents of these infections are Brucella ovis, Actinobacillus seminis, and Histophilus somni. The aim of this study was to develop a multiplex PCR assay for simultaneous detection of Brucella ovis, Actinobacillus seminis, and Histophilus somni with species-specific primers applied to biological samples for molecular diagnosis of these infections.ResultsThe multiplex assay was capable of detecting B. ovis, A. seminis, and H. somni DNA simultaneously from genomic bacterial DNA samples and pool of semen samples from experimentally infected rams. The method was highly specific since it did not amplify DNA from other bacterial species that can potentially cause epididymitis in rams as well as species phylogenetically related to B. ovis. All negative control samples were negative in PCR multiplex assay. Urine can be used as an alternative to semen samples.ConclusionsThe species-specific multiplex PCR assay developed in this study can be successfully used for the detection of three of the most common bacterial causes of ovine epididymitis.

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Diagnosis of Brucella ovis infection by serology and PCR in urine samples from naturally infected rams in the state of Piauí

Abstract: Communication [Comunicação] Diagnosis of

Cited by 15 publications

References 9 publications

Encapsulated Brucella ovis Lacking a Putative ATP-Binding Cassette Transporter (ΔabcBA) Protects against Wild Type Brucella ovis in Rams

Encapsulated Brucella ovis Lacking a Putative ATP-Binding Cassette Transporter (ΔabcBA) Protects against Wild Type Brucella ovis in Rams

Serologic and molecular evidence of Brucella ovis infection in ovine and caprine flocks in the State of Minas Gerais, Brazil

Species-specific multiplex PCR for the diagnosis of Brucella ovis, Actinobacillus seminis, and Histophilus somni infection in rams

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