Efeito do número de inseminações artificiais sobre a fertilidade de éguas inseminadas com sêmen asinino diluído e resfriado a 5ºC por 12 horas de armazenamento

Rossi, Robson Marcelo; Filho, J.M. Silva; Palhares, Maristela Silveira; Ribeiro, Eduardo Magalhães; Resende, Y. F.; Anjos, Filomena Rosa Dos

doi:10.1590/s0102-09352012000100017

Cited by 2 publications

(3 citation statements)

References 10 publications

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“…The previous studies using egg yolk in equine semen extenders were equivocal. Some studies (Province et al, 1984;Bruemmer et al, 2002) showed inferiority of egg yolk-based extenders and others (Rota et al, 2004;Melo et al, 2005;Rota et al, 2008) showed superiority of yolk-based extenders on the motility and viability at different periods of storage in comparison with those from milk-based extenders.…”

Section: Resultsmentioning

confidence: 99%

“…A glycine egg yolk-based extender (Foote, 2002) was used for cooling of boar semen and the sperm motility did not decline more than 10% during 2 days of storage at various temperatures (5, 15 or 25 °C). The same extender maintained the fertility of donkey semen after dilution and cooling at 5 °C for 12 hours (Rossi et al, 2012), showing promising results for adaptation for use in cooling of stallion semen.…”

Section: Introductionmentioning

confidence: 83%

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Viability and fertility of cooled equine semen diluted with skimmed milk or glycine egg yolk-based extenders

et al. 2012

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-Two semen extenders were compared for their ability to maintain viability of horse semen during 24 hours of cold preservation, and for the pregnancy rate after artificial insemination. In the experiment 1, five ejaculates from three stallions were split-diluted in either a skimmed milk-based extender (Kenney extender) or a glycine egg yolk-based extender (Foote extender) and cooled at 6-8 °C for 24 hours. Semen samples stored in Kenney extender for 24 hours had higher motility and spermatic vigor compared with those stored in Foote extender. However, samples stored in Foote extender had higher number of reactive sperm by hypoosmotic test and greater viability by epifluorescence test compared with those in Kenney extender. In the experiment 2, 17 and 23 ejaculates from two stallions were split-diluted with Kenney extender and Foote extender. The sperm concentration in each extender was adjusted to 500 million viable sperms per insemination dose. Semen was cooled to 6-8 °C and stored for 24 hours. Seventy-four cycles of crossbred mares were inseminated with either semen diluted in Kenney extender or semen diluted in Foote extender. The pregnancy rate was higher from semen diluted in Kenney extender than that from semen in Foote extender (0.553 vs. 0.306). The Kenney extender is effective in preserving the motility, vigor and fertility of stallion semen after 24 hours of cold storage, whereas the Foote extender is not acceptable.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 83%

Viability and fertility of cooled equine semen diluted with skimmed milk or glycine egg yolk-based extenders

et al. 2012

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“…Sin embargo, diferentes estudios han demostrado que a pesar de contar con semen criopreservado de excelente calidad, su fertilidad en la inseminación de asnas es reducida (Oliveira et al, 2016). Lo que ha llevado a la búsqueda de alternativas que permitan mejorar la respuesta uterina y las estrategias para la inseminación con semen criopreservado (Jepsen et al, 2010;Rossi et al, 2012;Miró et al, 2013). Por lo anterior, y a partir de los resultados de esta investigación, se plantea la necesidad de evaluar la contribución de los suplementos empleados en el semen criopreservado mediante pruebas de fertilidad in vivo.…”

Section: Parámetrounclassified

Efecto de la suplementación del diluyente sobre la calidad del semen de asno a la descongelación

2017

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Palabras clave adicionalesCalidad seminal. Crioprotectores. Plasma seminal. Yema de huevo. Criopreservación. resUMenLa congelación de semen de asno permite almacenar material genético por un periodo ilimitado de tiempo, por lo que puede ser empleado para generar bancos de germoplasma o para la cría de mulares y asnos. Esta investigación tuvo como objetivo evaluar alternativas de suplementación de un diluyente para la congelación de semen de asno. Se colectó el semen de cinco asnos criollos colombianos, dos eyaculados por animal (n= 10) y luego se congeló en diluyente Equiplus® suplementado con 3% de glicerol (GLY) ó 5% de dimetilformamida (DMF), 2% ó 5% de yema de huevo centrifugada (YHC) y 10% ó 20% de plasma seminal (PS). Se realizó la evaluación post-descongelación de la calidad seminal y de la peroxidación lipídica (PL). Para el análisis estadístico se ajustaron modelos mixtos, se realizó un análisis de regresión y comparaciones de medias por la prueba de Tukey. El semen congelado con 5% de DMF, 5% de YHC y 10% de PS presentó valores superiores de movilidad, vitalidad e integridad de membrana (p<0,05). La PL fue menor en diluyentes con 5% de YHC (p<0,05). La suplementación del diluyente de congelación con una combinación de 5% de DMF, 5% de YHC y 10% de PS permite mejorar la calidad post-descongelación del semen de asno. Effect of extender supplementation on the post-thaw semen quality of donkey sUMMarY Freezing of donkey semen allows storing genetic material for an unlimited period of time, which can be used to generate germoplasm banks or for breeding of mules or asses. This research aimed to evaluate supplementation alternatives for freezing donkey semen. Semen from five Colombian creole donkeys (n= 10) was collected and then frozen in Equiplus® extender supplemented with 3% of glycerol (GLY) or 5% of dimethylformamide (DMF), 2% or 5% of centrifuged egg yolk (CEY) and 10% or 20% of seminal plasma (SP). Post-thaw evaluation of semen quality and lipid peroxidation (LP) was conducted. For statistical analysis mixed models were adjusted, regression analysis and comparison of means by the Tukey test were performed. Frozen semen with DMF, 5% CEY and 10% SP presented superior results of motility and membrane integrity (p<0.05). The LP was lower in extenders with 5% CEY (p<0.05). Supplementation of freezing extender with the combination of 5% DMF, 5% CEY and 10% SP allows to improve the post-thaw quality of asinine semen.additional keYwords

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Efeito do número de inseminações artificiais sobre a fertilidade de éguas inseminadas com sêmen asinino diluído e resfriado a 5ºC por 12 horas de armazenamento

Cited by 2 publications

References 10 publications

Viability and fertility of cooled equine semen diluted with skimmed milk or glycine egg yolk-based extenders

Viability and fertility of cooled equine semen diluted with skimmed milk or glycine egg yolk-based extenders

Efecto de la suplementación del diluyente sobre la calidad del semen de asno a la descongelación

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