Avaliação clínica da infecção experimental de bezerros com Salmonella Typhimurium

Ávila, Larissa Gabriela; Silva, Daniela Gomes da; Sato, Rafael Akira; Fagliari, José Jurandir

doi:10.1590/s0102-09352011000600043

Cited by 3 publications

(2 citation statements)

References 7 publications

(6 reference statements)

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“…The results of these experiments indicated all 3 serotypes of Salmonella persist in bedding sand for extended periods of time. The colony-forming units per gram levels in our experiments are within the predicted infectious dose for cattle, which is believed to follow ingestion of 10 4 to 10 11 cfu ( Wray and Sojka, 1978 ; Wray and Davies, 2000 ; Ávila et al, 2011 ). In general, numbers of Salmonella in the sterile sand remained constant or increased slightly during the first 3 d, and then remained at that level or decreased somewhat through 28 d. There was some variation in the temporal response of the 3 serotypes.…”

supporting

confidence: 71%

Survival of bovine-associated serotypes of Salmonella enterica in bedding sand

Pilch

Aulik²,

Sockett³

et al. 2023

JDS Communications

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Graphical Abstract Summary: Salmonella enterica sv. Dublin inoculated into sterile bedding sand can survive and proliferate when incubated at room temperature for up to 7 days. However, inoculation of Salmonella enterica sv. Dublin into bedding sand that contains a naturally occurring microbiota results in a decrease in cfu of Salmonella enterica sv. Dublin under the same conditions.

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supporting

confidence: 71%

Survival of bovine-associated serotypes of Salmonella enterica in bedding sand

Pilch

Aulik²,

Sockett³

et al. 2023

JDS Communications

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“…A maioria dos estudos sobre a ocorrência e a etiopatogenia da salmonelose em ruminantes é relacionada à espécie bovina. Nesta espécie, a salmonelose é causada principalmente por S. enterica subspécie enterica sorotipo Dublin (S. Dublin) e S. enterica subspécie enterica sorotipo Typhimurium (S. Typhimurium), sendo S. Dublin o sorotipo adaptado à espécie bovina (Wray e Davies, 2000;Santos et al, 2002;Silva et al, 2008;Carrique-Mas et al, 2010;Ávila et al, 2011).…”

Section: Salmonelose Bovinaunclassified

Salmonelose

Silva¹

2017

Rev. Acad. Ciên. Anim.

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A salmonelose é uma enfermidade infecciosa causada por bactérias do gênero Salmonella, responsável por grandes prejuízos na produção animal e de grande importância em saúde pública, uma vez que é considerada uma das principais enfermidades de origem alimentar relacionadas ao consumo de produtos de origem animal contaminados. A salmonelose é de ocorrência cosmopolita, sendo a bactéria encontrada em animais de sangue quente e frio (CDC, 2015; Strockbine et al., 2015). As salmonelas são bactérias Gram-negativas, intracelulares facultativas, não-formadoras de esporos, aeróbias ou anaeróbias facultativas, geralmente móveis, pertencentes à família Enterobacteriaceae. Apesar das constantes modificações taxonômicas ocorridas nos últimos anos, a classificação mais utilizada sugere a existência de duas espécies: Salmonella enterica (subdividida em seis subespécies: enterica, salamae, arizonae, diarizonae, houtenae e indica) e Salmonella bongori, com mais de 2.500 sorotipos conhecidos. Entretanto, somente os sorotipos de S. enterica subespécie enterica infectam animais de sangue quente.

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Blood serum acute phase proteins and iron dynamics during acute phase response of Salmonella enterica serotype Dublin experimentally infected buffalo calves

Santana

Silva

Thomas

et al. 2018

Veterinary Immunology and Immunopathology

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The study aimed to evaluate clinical signs, blood serum acute phase proteins (APP) and iron dynamics during the acute phase response (APR) of Salmonella Dublin experimentally infected Murrah buffalo calves. Six buffalo calves constituted the control group (CNT) and six were orally inoculate with 10 CFU of S. Dublin (INF). Clinical evaluation was performed, rectal swabs to detect S. Dublin strains were collected and venous blood was sampled before and throughout seven days after inoculation. The APP fractions β-haptoglobin, α-haptoglobin, ceruloplasmin and transferrin were analyzed by 1-D and 2-D electrophoresis. Proteins were identified using LC/ESI-MS/MS and NCBI database. Plasma fibrinogen, serum iron and serum haptoglobin concentrations were measured. The inoculation of 10 CFU of S. Dublin was effective in inducing clinical signs of Salmonellosis, such as hyperthermia and diarrhea. 1-DE showed that β and α-haptoglobin increased 204% (p = 0.008) and 184% (p = 0.022) 48 h after inoculation (HAI), respectively, with highest concentrations 120 HAI (498% increased, p = 0.012; 431% increased, p = 0.011) and 168 HAI (492% increased, p = 0.019; 523% increased, p = 0.028). 2-DE showed that the expression of two spots, identified as β-haptoglobin, were increased 693% (p = 0.0006) and 580% (p = 0.0003) 168 HAI, respectively, while one spot, identified as α-haptoglobin, increased 714% (p = 0.040). Haptoglobin concentrations increased 1339% (p < 0.0001) 168 HAI. 1-DE showed that ceruloplasmin increased 42% (p = 0.034) 48 HAI, with highest concentration 120 HAI (133% increased, p = 0.022). 2-DE showed that the expression of two spots, identified as ceruloplasmin, were increased 218% (p = 0.0153) and 85% (p = 0.0143) 168 HAI, respectively. Fibrinogen increased 78% (p = 0.012) 96 HAI, with highest concentration 120 HAI (increased 114%, p = 0.002). Iron decreased 33% 24 HAI (p = 0.015) and 37% 72 HAI (p = 0.029), and began to be restored 96 HAI. 1-DE showed that transferrin decreased 23% 120 HAI (p = 0.047), and that values were restored 168 HAI. 2-DE showed that expression patterns of transferrin comparing 0 h and 168 HAI were similar, evidencing that values were restored 168 HAI. In conclusion, the inoculation of 10 CFU was effective in inducing hyperthermia and diahrrea. β and α-haptoglobin, ceruloplasmin and fibrinogen worked as positive APP during the APR to S. Dublin infection and are potential biomarker candidates. Concentrations of iron and transferrin decreased during the infection, highlighting the fact that mechanisms for restricting iron availability are part of the APR triggered against S. Dublin infection in buffalo calves.

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Avaliação clínica da infecção experimental de bezerros com Salmonella Typhimurium

Cited by 3 publications

References 7 publications

Survival of bovine-associated serotypes of Salmonella enterica in bedding sand

Survival of bovine-associated serotypes of Salmonella enterica in bedding sand

Salmonelose

Blood serum acute phase proteins and iron dynamics during acute phase response of Salmonella enterica serotype Dublin experimentally infected buffalo calves

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