Óxido nítrico, GSTP-1 e p53: qual o papel desses biomarcadores nas lesões prostáticas do cão?

Croce, G; Rodrigues, Marcela Marcondes Pinto; Faleiro, Mariana Batista Rodrigues; Moura, Veridiana Maria Brianezi Dignani de; Amorim, Renée Laufer

doi:10.1590/s0102-09352011000600013

Cited by 3 publications

(3 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…There were differences between PIA and PC, but not between PC and PIN relative to the number of stained cells. These results suggest that alterations related with the p53 protein begin in the canine prostate with PIA and are accentuated in the canine glands with PIN and PC, reinforcing malignant potential of PIN as previously described in dogs (CROCE et al, 2011), and humans (STANGELBERGER et al, 2012). According to Stangelberger et al (2012), the high expression of p53 in human PC and PIN has been associated with progression of disease due to higher proliferative indices and more aggressive phenotype.…”

supporting

confidence: 82%

See 1 more Smart Citation

Immunoexpression of cell cycle regulators in canine prostate with proliferative lesions

Faleiro¹,

Silva²,

Moura³

2018

SCA

View full text Add to dashboard Cite

Immunostaining of p21, p27, p53, cyclin D1, c-myc was evaluated in normal canine prostate and prostate with proliferative disorders to verify the interaction between these regulators of cell cycle progression. From 106 samples of canine prostate obtained from a TMA block, 15 were considered normal, 16 diagnosed as benign prostatic hyperplasia (BPH), 30 as proliferative inflammatory atrophy (PIA), 20 as prostatic intraepithelial neoplasia (PIN), and 25 as prostatic carcinoma (PC). There was positive correlation between p21 and p27 for number of stained cells and staining intensity in all conditions and between c-myc and p53 in prostates with PIN. Considering the number of labeled cells, there was positive correlation between p21 and p53 in the normal prostate. Relative to the intensity of staining, there was positive correlation between p21 and p53 in prostate tissue with PIN and between p27 and c-myc in prostates with PIA. A negative correlation between c-myc and cyclin D1 was also identified in the glands with PIN, considering the number of labeled cells, and between p27 and c-myc in the prostates with PC for staining intensity. In conclusion, the expression of p21, p27, p53, cyclin D1 and c-myc varies according to type of proliferative lesion in canine prostate. Taken together, the results indicate low growth potential of the canine PC in the presence of p21 and p27 overexpression, cyclin D1 low expression and regular expression of c-myc, even with the expression of p53 mutant type. Further, it was possible reaffirm the premalignant potential of PIA and PIN in canine prostate.

show abstract

supporting

confidence: 82%

“…The expression of p53 was observed in normal canine prostate, in proliferative lesions, and a higher expression in PC, as reported by Croce et al (2011). There were differences between PIA and PC, but not between PC and PIN relative to the number of stained cells.…”

supporting

confidence: 76%

Immunoexpression of cell cycle regulators in canine prostate with proliferative lesions

Faleiro¹,

Silva²,

Moura³

2018

SCA

View full text Add to dashboard Cite

show abstract

“…PIN is considered the main precursor lesion of prostate cancer in men, mainly high-grade, and often diagnosed as a lesion adjacent to prostate adenocarcinoma [ 39 , 40 ]. On the other hand, PIN has a low occurrence in intact dogs and, when present, tends to present as a focal lesion [ 41 ]. Its actual incidence is still considered questionable and probably overestimated or not correctly diagnosed in many studies, besides its role in canine PC carcinogenesis still to be discussed [ 33 ].…”

Section: Preneoplastic Prostatic Lesionsmentioning

confidence: 99%

Comparative Pathobiology of Canine and Human Prostate Cancer: State of the Art and Future Directions

Nascente

Amorim

Fonseca-Alves

et al. 2022

Cancers

View full text Add to dashboard Cite

First described in 1817, prostate cancer is considered a complex neoplastic entity, and one of the main causes of death in men in the western world. In dogs, prostatic carcinoma (PC) exhibits undifferentiated morphology with different phenotypes, is hormonally independent of aggressive character, and has high rates of metastasis to different organs. Although in humans, the risk factors for tumor development are known, in dogs, this scenario is still unclear, especially regarding castration. Therefore, with the advent of molecular biology, studies were and are carried out with the aim of identifying the main molecular mechanisms and signaling pathways involved in the carcinogenesis and progression of canine PC, aiming to identify potential biomarkers for diagnosis, prognosis, and targeted treatment. However, there are extensive gaps to be filled, especially when considering the dog as experimental model for the study of this neoplasm in humans. Thus, due to the complexity of the subject, the objective of this review is to present the main pathobiological aspects of canine PC from a comparative point of view to the same neoplasm in the human species, addressing the historical context and current understanding in the scientific field.

show abstract

Expression of cell cycle inhibitors in canine prostate with proliferative inflammatory atrophy and carcinoma

Faleiro¹,

Cintra

Jesuino

et al. 2018

Arq. Bras. Med. Vet. Zootec.

Self Cite

View full text Add to dashboard Cite

Gene expression of CDKN1A, CDKN1B, and TP53, and immunostaining of p21, p27 and p53 were evaluated to verify the role of these cell cycle inhibitors in canine prostates with proliferative inflammatory atrophy-PIA and prostatic carcinoma-PC. Seventy samples, 15 normal, 30PIA and 25PC. Regarding number of p27 and p53 labeled cells, difference between normal and PIA and PC was observed, as well as between PIA and PC for p53. Immunostaining intensities of p21, p27 and p53 were different when comparing normal tissues to PIA and PC. Sixteen cDNA of canine prostatic FFPE tissue were subjected to RT-PCR and RT-qPCR, four normal, three PIA, and nine PC. CDKN1A mRNA was detected in four PC by RT-PCR, and it was overexpressed when compared to normal by RT-qPCR, in one PIA and six PC. CDKN1B mRNA was detected in three PC by RT-PCR and it was overexpressed in three PC and decreased in one PC. TP53 mRNA was overexpressed in one PIA and three PC. In conclusion, when overexpressed in canine prostate with premalignant and malignant, p21 and p27 play a role controlling cell proliferation, working as a protective factor in the evolution of PIA to PC, and in the PC development, even in the presence of altered p53. RESUMOA expressão gênica de CDKN1A, CDKN1B e TP53, assim como imunomarcação de p21, p27 e p53 foram realizadas a fim de verificar o papel desses inibidores do ciclo celular na próstata canina com atrofia inflamatória proliferativa (PIA) e carcinoma prostático (PC). Foram obtidas70 amostras de próstata canina, sendo 15 de tecido normal, 30 de PIA e 25 de PC. Quanto ao número de células imunomarcadas foi observada diferença entre amostras normais, com PIA e PC para p27 e p53, assim como entre PIA e PC para p53. Para a intensidade de imunomarcação houve diferença entre os tecidos normais e com PIA e PC para p21, p27 e p53. Foram obtidas dezesseis amostras de cDNA a partir de amostras de próstatas caninas embebidas em parafina para a realização da RT-PCR e RT-qPCR, sendo quatro normais, três com PIA, e nove com o PC. O gene CDKN1A foi detectado em quatro das amostras com PC por RT-PCR, e pela RT-qPCR este estava superexpresso em uma PIA e em seis PC quando da comparação com o tecido prostático normal. O CDKN1B foi detectado em três PC por RT-PCR e pela RT-qPCR estava superexpresso em três PC e reduzido em um PC. O TP53 foi detectado em todas as próstatas caninas com PIA e PC por RT-PCR, sendo também superexpresso em uma glândula com PIA e em três com PC. Concluiu-se que p21 e p27 quando superexpressas na próstata canina com lesões pré-malignas (PIA) e malignas (PC) desempenham ação no controle da proliferação celular, possivelmente atuando como fator de proteção na evolução da PIA para PC, e no desenvolvimento do PC, mesmo na presença de p53 alterada. Assim, o próximo passo é avaliar essas proteínas do ciclo celular em casos de PC canino com metástase. p27, p53, PIA, Recebido em 28 de setembro de 2016 Aceito em 15 de março de 2017 

show abstract

Óxido nítrico, GSTP-1 e p53: qual o papel desses biomarcadores nas lesões prostáticas do cão?

Cited by 3 publications

References 20 publications

Immunoexpression of cell cycle regulators in canine prostate with proliferative lesions

Immunoexpression of cell cycle regulators in canine prostate with proliferative lesions

Comparative Pathobiology of Canine and Human Prostate Cancer: State of the Art and Future Directions

Expression of cell cycle inhibitors in canine prostate with proliferative inflammatory atrophy and carcinoma

Contact Info

Product

Resources

About