Transference in vitro of the resistance to the antimicrobials between Escherichia coli, Lactobacillus spp. and Salmonella enteritidis isolated from chickens

Abstract

The objective of this work was to verify the possibility of transference of resistance to the antimicrobials between bacteria that are in the present normal microbiota of chickens and Salmonella Enteritidis. Samples of Lactobacillus spp. (L. spp.), Salmonella Enteritidis (SE) and Escherichia coli (E. coli) previously isolated from chickens, selected after the test of sensitivity antimicrobial in vitro according the standard method (National Committee for Clinical Laboratory Standards) utilizing those with resistance and sensibility to the antimicrobials inductors, named donor and receptor bacteria, respectively were used. Antimicrobials inductors were utilized to stimulate the transference of resistance to the antimicrobials between the bacteria. The possibility of transference was verified from the E. coli resistant to the SE and L. spp. Transference of a sample of L. spp resistant to the antimicrobials inductors to the SE was also verified. It was only possible to verify the transference of the resistance to the antimicrobials inductor when the donor bacteria was the E. coli and the bacteria receptor was SE. In the present study we conclude that the transference of resistance to the antimicrobials between bacteria is possible, however, not all bacteria participate in that trial, not transmitting and neither acquiring this resistance.

Microbioma intestinal das aves e sua importância

“…As espécies do gênero Lactobacillus que abrigam no segmento intestinal das aves são Lactobacillus salivarius, Lactobacillus aviarius, Lactobacillus fermentum, Lactobacillus acidophilus e Lactobacillus reuteri (Okamoto, et al, 2011); (Paixão & Castro, 2016). Os Lactobacillus sobrevivem sob o pH baixo do proventrículo, inibi o crescimento de Campylobacter em aves, principalmente as espécies L. salivarius e L. reuteri.…”