Comparação entre dois protocolos para obtenção de plasma rico em plaquetas, em cães

Aleixo, G. A. S.; Coelho, Mariana de Freitas; Teixeira, Miriam Nogueira; Mesquita, Emanuela Polimeni de; Oliveira, Flaviano Fernandes de; Zubieta, L.M.V.; Almeida, Telga L.C. de; Guimarães, Ana Luiza Neves; Maia, Frederico Celso Lyra; Zacarias, T.F.L.; Santos, Seth M.; Lima, Cruvinel

doi:10.1590/s0102-09352011000300005

Cited by 6 publications

(8 citation statements)

References 15 publications

(7 reference statements)

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“…In order to obtain PRP, we performed double centrifugation with adequate rotation force to avoid the premature release of the GF in the platelets 20 , and thus, promoted its action in the wound repair process. Platelet activation by the addition of 10% calcium gluconate immediately prior to the treatment of these lesions ensured, as indicated by Maia et al 9 , a suitable concentration of these factors in the PRP, the most important of which are TGF-β, PDGF, and IGF-I 4 .…”

Section: Discussionmentioning

confidence: 99%

Effects of heterologous platelet-rich plasma gel on standardized dermal wound healing in rabbits

et al. 2015

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Intellectual and scientific content of the study, surgical procedures, critical revision. ABSTRACT PURPOSE:To evaluate the potential of heterologous platelet-rich plasma (PRP) gel for surgical skin wound healing in rabbits METHODS: Blood from a single healthy dog was used for PRP production, with calcium gluconate added to the PRP to form the gel.Two surgical excisions, one to the right and the other to the left of the dorsal midline, were made in six rabbits. One side was randomly allocated to topical application of a physiological solution, and the other was allocated to treatment with heterologous PRP gel. Clinical assessments (weight, pain sensitivity, coloring, edema, hyperemia, exudation, crust, and granulation) and morphometric evaluations were performed 0, 3, 7, 10, 14, and 17 days postoperatively. Histological analysis was performed on the 17 th day. RESULTS:With the exception of the presence of a crust at day 10, clinical variables did not differ significantly between the experimental groups. In both the control and PRP-treated groups, differences were identified when comparing time-points in terms of wound area reduction. Histological results indicated no significant differences between the control group and the PRP-treated group. CONCLUSION:Heterologous platelet-rich plasma gel promoted dermal wound healing in rabbits with no adverse effects.

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Section: Discussionmentioning

confidence: 99%

Effects of heterologous platelet-rich plasma gel on standardized dermal wound healing in rabbits

et al. 2015

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“…The protocols were selected for being simple, low-cost, and efficient at concentrating platelets (ALEIXO et al, 2011;KIM et al, 2002). However, leukocytes and red blood cells were not evaluated in previous studies.…”

Section: Resultsmentioning

confidence: 99%

“…The microscopic morphological visualization of platelets using a PRP smear is recommended to evaluate the quality of the blood component, since pseudopods are formed after activation (WEISS; WARDROP, 2010), releasing growth factors in advance (EPPLEY et al, 2004). A previous study showed no morphological changes in PRP platelets obtained by direct microscopy (ALEIXO et al, 2011). However, a study using electronic microscopy to visualize platelet morphology in PRP detected 49% of platelets with uncertain activation, 41% at rest, 9% activated, and 1% damaged (ZANDIN et al, 2012).…”

Section: Resultsmentioning

confidence: 99%

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Comparison of the protocols for obtaining platelet-rich plasma in dogs: a cellular study

et al. 2020

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This study aimed to evaluate two protocols (PA and PB) that are used to obtain canine platelet-rich plasma (PRP) for cellularity. Twenty healthy dogs were used. Blood samples were collected and placed in two tubes of 3.2% sodium citrate. PA used double centrifugation at 210 x g, and 370 x g and PB used double centrifugation with 140 x g and 330 x g. The PRP samples from the protocols were examined in terms of their platelet, erythrocyte, and leukocyte count in the Neubauer chamber, differential leukocyte count and platelet morphological observation in blood smears. Data (mean and standard deviation) were analyzed with the 95% probability t-test (P <0.05) using Pearson’s correlation to test the relationship between platelets and erythrocytes, platelets, and leukocytes, and the leukocyte count versus the erythrocytes. Very weak negative correlation between platelets and leukocytes (p= -0.03), weak negative correlation between platelets and erythrocytes (p= -0.3) and a strong positive correlation between leukocytes and erythrocytes (ρ = 0.75) were noted. Although, BP did not reach the desired mean of one million platelets (979300 ± 79631 cells / μL), both protocols, A and B (4.42 ± 1.61 and 3.85 ± 1.55 times more platelets than total blood, respectively) (p <0.05) were efficient in concentrating platelets. Platelet activation was present in 26.55 ± 6.72% of the PA platelets and 26.25 ± 7.03% in PB (p> 0.05). PA and PB presented low erythrocyte concentration (p> 0.05), and PA had more leukocytes (p <0.05) than PB, with higher concentrations of basophils that were segmented, and lymphocytes.

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“…Na medicina humana, estão disponíveis vários sistemas de separação comercial para a preparação do PRP, porém as concentrações de componentes sanguíneos sofrem variações substanciais entre esses sistemas (OUDELAAR et al, 2019). Na veterinária, não há uma padronização da técnica para a produção de PRP em cães e diferentes métodos têm sido utilizados visando encontrar uma melhor concentração com a preservação das propriedades plaquetárias (FERRAZ et al, 2007;ALEIXO et al, 2011;VANAT et al, 2012, PENHA et al, 2014.…”

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Comparação De Diferentes Protocolos Para Obtenção De Plasma Rico Em Plaquetas Canino

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Rocha

Lira

et al. 2020

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O plasma rico em plaquetas (PRP) é um hemocomponente produzindo a partir do plasma, visando a obtenção de citocinas e fatores do crescimento com fins terapêuticos, como a cicatrização e reparação de diferentes tecidos. Esse estudo quantificou a concentração plaquetária obtida utilizando-se de três diferentes protocolos de obtenção do PRP de 13 amostras de sangue de 7 cães hígidos. As velocidades de centrifugação utilizadas no protocolo I foram de 1ª centrifugação de 1.500 rpm e 2ª centrifugação de 3.000 rpm; no protocolo II, de 2.000 rpm e 3.000 rpm; e o protocolo III com 3.000 rpm e 4.000 rpm, respectivamente. O melhor resultado para a produção de PRP autólogo foi obtido no protocolo 1, seguido do protocolo 2, com aumentos de 116% e 83,05% após a 2ª centrifugação, respectivamente. Entretanto, os resultados obtidos na 1ª centrifugação foram superiores aos apresentados após a 2ª. centrifugação, observando elevações de 203,66% e 210,20% comparados aos valores basais.

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Comparação entre dois protocolos para obtenção de plasma rico em plaquetas, em cães

Cited by 6 publications

References 15 publications

Effects of heterologous platelet-rich plasma gel on standardized dermal wound healing in rabbits

Effects of heterologous platelet-rich plasma gel on standardized dermal wound healing in rabbits

Comparison of the protocols for obtaining platelet-rich plasma in dogs: a cellular study

Comparação De Diferentes Protocolos Para Obtenção De Plasma Rico Em Plaquetas Canino

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