Quantificação de transcritos maternos em oócitos bovinos submetidos a diferentes condições de maturação

Munk, Michele; Costa, Fabiano Freire; Oliveira, André Alves de; Serapião, R. V.; Machado, M. A.; Viana, J. H. M.; Camargo, L. S. A.

doi:10.1590/s0102-09352010000600015

Cited by 4 publications

(4 citation statements)

References 27 publications

(3 reference statements)

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“…In a study conducted by (Wu et al, 1996;) and (Tong et al, 2000) with mice, it was possible to observe that although in the absence MATER adequate follicular development occurs, ovulation and fertilization with normal zygotes, embryos obtained do not develop beyond the 2-cell stage and tend to degenerate. Therefore, the block on the development observed in embryos cultured in vitro, which coincides with the EGA, are possibly related with the least amount of MATER reported in bovine oocytes matured in vitro (Pereira et al, 2010). This result is similar to our study, where concentrations of T150 T200 showed the smallest quantities of transcripts and subsequently lower rates of cleavage and blastocyst formation.…”

Section: Arq Brassupporting

confidence: 90%

Effect of Rolipram on in vitro maturation, gene expression and embryonic development in bovines

Santana

Sobral

Gomes

et al. 2019

Arq. Bras. Med. Vet. Zootec.

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The aim of this work was to evaluate the effect of the Rolipram during the maturation of bovine oocytes and gene expression of embryos produced in vitro. Bovine ovaries were collected in slaughterhouse. The COCs were selected and divided into 5 groups: Control 0 time; Control: IVM for 24 hours; Rolipram treatments with IVM blocking for 24 hours in maturation medium containing (100, 150 and 200µM). After 24 hours all groups were reseated in IVM for another 24 hours. Subsequently COCs were subjected to the same IVM system and fertilized, being checked for cleavage post fertilization and for blastocyst. In addition, performed expression of the following genes: Mater, BMP15 and Bax. No difference was found in gene expression. Of oocytes evaluated shortly after follicular aspiration, 79.00% were in GV, GVBD, MI, while 13.40%, were in MII and 7.60%, D/NI. Significant difference was observed in different concentrations (T100, T200 and T150µM) in oocytes that have reached the MII phase compared to control treatments (P= 0.003). Differences were observed in cleavage rate (P< 0.05) between T150 and T200 when compared to the C/24 Group. A high difference was observed on blastocyst rate (P< 0.001) among treatments compared to the control group.

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Section: Arq Brassupporting

confidence: 90%

Effect of Rolipram on in vitro maturation, gene expression and embryonic development in bovines

Santana

Sobral

Gomes

et al. 2019

Arq. Bras. Med. Vet. Zootec.

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“…The nuclear maturation rates achieved at 28 h of maturation in media containing high concentrations of FF (75 or 100%) were greater than those observed in other maturation times, possibly due to the delay of meiotic progression caused by FF, which provided additional time for the oocytes to acquire competence (Pereira et al, 2010;Prentice et al, 2011;Gottardi et al, 2012). According to Aguilar et al (2001), a slower nuclear maturation provides oocytes with more time to synthesize, modify and store new proteins and ribonucleoproteins, and therefore enhances their competence.…”

Section: Resultsmentioning

confidence: 95%

“…Considering that the major nuclear changes occur during oocyte maturation, the assessment of nuclear maturation rate is a useful tool to evaluate oocyte competence (Pereira et al, 2010;Prentice et al, 2011). In horses, Dell 'Aquila et al (1997) and Bøgh et al (2002), using 100% FF, found nuclear maturation rates of 68.5 and 79%, respectively.…”

Section: Resultsmentioning

confidence: 99%

Effect of follicular fluid supplementation during in vitro maturation on total cell number in bovine blastocysts produced in vitro

et al. 2014

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-This study evaluated the influence of follicular fluid (FF) added to the maturation medium on the quality of bovine embryos produced in vitro. In the first experiment, oocytes were matured in media containing five different FF concentrations with different maturation times and classified according to meiotic progression and migration of cortical granules. In the second experiment, oocytes matured in the same media were fertilized at three different maturation times; thereafter, cleavage and blastocyst rates were evaluated. In the third experiment, oocytes were matured in media containing three different FF concentrations at two different maturation times, and embryo quality, inferred by the ratio of inner cell mass and trophectoderm cells compared with total cell number, was evaluated. Higher FF concentration (75 -100% FF) slowed meiotic progression and CG migration (control -78.13% vs. treated -52.58% and control -52.7% vs. treated -11.59%, respectively, at 24 h of maturation). Also, FF at concentration of 75% or 100% had a negative influence on cleavage and blastocyst rates (control -90.13% vs. treated -82.64% and control -35.73% vs. treated -11.57%, respectively, at 24 h of maturation). The 50% FF resulted in embryos with increased inner cell mass numbers (control -29.91 vs. treated -35.49, at 24 h of maturation) and total cell numbers (control -109.53 vs. treated -120.67, at 26 h of maturation). Even though higher concentration of FF added to the maturation medium reduced embryonic development rates, in lower concentrations, FF slowed the meiotic progression and migration of CG and contributed to increases in inner cell mass number. Thus, FF added to the maturation medium enhances the number of cells in bovine embryos produced in vitro, especially for inner cell mass.

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“…In vitro embryo production (IVEP) has been prominent over the years, especially for domestic animals, such as cattle (Boruszewska et al, 2016). Nevertheless, IVEP its efficiency is limited; it can generate about 30-50% of viable blastocysts (Gonçalves et al, 2007;Pereira et al, 2010). Thus, improvements are required in the culture conditions of various stages of IVEP, such as oocyte recovery, in vitro maturation (IVM) of selected oocytes, and fertilization (IVF) and development (IVD) of presumed zygotes to blastocyst stage to be transferred or cryopreserved (Varago et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

Influence of different protein supplements on the recovery and in vitro maturation of bovine oocytes

Fernandes-França¹,

Oliveira-Santos²,

Oliveira-Lira³

et al. 2019

Rev. colomb. cienc. pecu.

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Background: Oocyte quality and maturation are influenced by protein supplementation. Objective: To evaluate the influence of fetal bovine serum (FBS) and bovine serum albumin (BSA) concentrations on the recovery and in vitro maturation (IVM) of bovine oocytes. Methods: The study was divided into Stage 1 (oocyte recovery), and Stage 2 (IVM). In the first stage, three experiments were conducted according to the recovery (R) medium used: (R1) 10 vs. 20% FBS; (R2) 5 vs. 10% BSA; and (R3) the best results from R1, R2, and the combination of FBS+BSA (5+5%). Within the second stage, the maturation medium was supplemented according to three experiments: (M1) 5 vs. 10% FBS; (M2) 0.4 vs. 0.8% BSA; and (M3) better results of M1, M2, and the combination of FBS+BSA (5+0.8%). Results: In Stage 1 (R1 and R2), the media with 10% FBS and 10% BSA showed better oocyte quality results and were defined for experiment R3. In R3, the 10% FBS and the combination of FBS+BSA (5+5%) allowed recovery of better-quality oocytes. In Stage 2 (M1 and M2), media with both levels of FBS (5 and 10%) and 0.8% BSA were defined as better according to the maturation and viability rates of cumulus cells, so they were defined for experiment M3. In M3, no difference was noted among the supplements. Conclusions: For oocyte recovery, 10% FBS and the combination of FBS+BSA (5+5%) can be used to obtain immature oocytes. For the in vitro maturation, FBS (both levels, 5 and 10%) and BSA (0.8%) can be used alone or in combination.

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Quantificação de transcritos maternos em oócitos bovinos submetidos a diferentes condições de maturação

Abstract: RESUMOComparou-se a quantidade relativa de transcritos de origem materna entre oócitos bovinos maturados in vivo e maturados em diferentes condições in vitro.

Cited by 4 publications

References 27 publications

Effect of Rolipram on in vitro maturation, gene expression and embryonic development in bovines

Effect of Rolipram on in vitro maturation, gene expression and embryonic development in bovines

Effect of follicular fluid supplementation during in vitro maturation on total cell number in bovine blastocysts produced in vitro

Influence of different protein supplements on the recovery and in vitro maturation of bovine oocytes

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