Perfil sorológico para circovírus suíno tipo 2 em granjas comerciais de suínos no Brasil

Barbosa, Clara Nilce; Lobato, Zélia Inês Portela; Martins, Nélson Rodrigo da Silva; Nascimento, Evaldo

doi:10.1590/s0102-09352008000400006

Cited by 7 publications

(8 citation statements)

References 12 publications

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“…Our results showed a high relative frequency (RF) (81.74%) of seroreactive animals and farms (100%). Similar results were found by Mesu et al (2000) in Belgium and Barbosa et al (2008) in the South and Southeast of Brazil. In both studies, the authors detected antibodies to PCV2 in 100% of the analyzed commercial herds.…”

supporting

confidence: 87%

“…Serological research has shown a wide distribution of PCV2 in the world pig population (Magar, Muller, & Larochelle, 2000;Mesu, Labarque, Nauwynck, & Pensaert, 2000;Zhou et al, 2006). In Brazil, after the identification of PCV2 by Zanella, Morés, Schiochet and Trombetta (2001), viral reactivity has been demonstrated in the South and Southeast regions (Barbosa, Lobato, Martins, & Nascimento, 2008), but information about PCV2 is scarce in the Northeast region, and epidemiological studies are essential to promote prevention and control programs with emphasis on swine health. Several serological techniques are available for PCV2 antibody detection (Magar et al, 2000;Opriessnig et al, 2007), including immunoperoxidase monolayer assay (IPMA) (Rodriguez-Arrioja et al, 2000).…”

mentioning

confidence: 99%

“…The literature has shown that epidemiological characteristics of the pathogen associated with the modern swine production system guarantee a continuous exposure of animals to the virus. According to C. N. Barbosa et al (2008), PCV2 circulates at all stages of the production cycle. Rose et al (2012) described that the virus could be maintained within herds for years due to population dynamics in modern swine production systems.…”

mentioning

confidence: 99%

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Serological survey of porcine circovirus 2 antibodies in Northeastern Brazil by immunoperoxidase monolayer assay

Duque

Silva

Júnior

et al. 2020

SCA

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Porcine circovirus 2 (PCV2) specific antibodies were evaluated in pigs at 15 to 25 weeks of age by immunoperoxidase monolayer assay. Serum samples (n = 575) were obtained from 16 commercial farms located in six states of northeastern Brazil, including two in Ceará (n = 85), three in Rio Grande do Norte (n = 92), two in Paraíba (n = 49), three in Pernambuco (n = 107), two in Alagoas (n = 39), and four in Bahia (n = 203). Out of the 575 evaluated sera, 470 (81.74%) were reagents with antibody titers ranging from 20 to 5,120. All farms presented reactive animals. There was a statistically significant difference in reactivity proportions between the states of Bahia and Ceará (p < 0.05) and between Bahia and Rio Grande do Norte (p < 0.05). This study provides the first serological investigation for PCV2 in pigs from commercial farms in Northeastern Brazil. ResumoA presença de anticorpos específicos contra o circovirus suíno 2 (PCV2) foi avaliada em suínos com 15 a 25 semanas de idade pela técnica de imunoperoxidase em monocamada de células. As amostras de soros (n = 575) foram procedentes de 16 granjas comerciais localizadas em seis Estados da região Nordeste do Brasil, incluindo duas no Ceará (n = 85), três no Rio Grande do Norte (n = 92), duas na Paraíba (n=49), três em Pernambuco (n = 107), duas em Alagoas (n = 39) e quatro na Bahia (n = 203). Dos 575 soros avaliados, 470 (81,74%) foram reagentes com títulos de anticorpos que variaram de 20 até 5.120. Todas as granjas avaliadas apresentaram animais reagentes. Houve diferença estatística significante nas proporções de sororeatividade entre os Estados da Bahia e Ceará (p < 0,05) e entre os Estados Bahia e Rio Grande do Norte (p < 0,05). Este estudo mostra a primeira investigação sorológica para PCV2 em suínos de granjas comerciais da região Nordeste do Brasil. Palavras-chave: Circoviridae. Infecção. Soro. Suíno. Vírus.

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supporting

confidence: 87%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Serological survey of porcine circovirus 2 antibodies in Northeastern Brazil by immunoperoxidase monolayer assay

Duque

Silva

Júnior

et al. 2020

SCA

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“…Serological data show that PCV2 infection is common in the swine and wild boars populations worldwide. In several European and American countries, PCV2 infection is widespread in commercial swine and can occur in herds affected and unaffected by the PMWS (Opriessnig et al., ; Barbosa et al., ).…”

Section: Introductionmentioning

confidence: 99%

Development and Evaluation of Single-tube Nested PCR (STNPCR) for the Detection of Porcine Circovirus type 2 (PCV2)

Pontes

Barbosa

Jesus

et al. 2012

Transbound Emerg Dis

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Porcine circovirus 2 (PCV2) is a common virus in pig population and is associated with the postweaning multisystemic wasting disease (PMWS). In this study, it was developed and evaluated the single-tube nested PCR (STNPCR) method for the detection of PCV2 DNA. PCV2 reference controls and swine tissue samples were used, and primers were selected for targeting specific regions of the viral genome. In comparison of the methods, STNPCR was 10 times more sensitive than conventional PCR and showed the same sensitivity to nested PCR (NPCR), but with reduction in the risk of cross-contamination. In clinical application, 55 tissue samples were analysed by conventional PCR and resulted in 67% (37/55) of positive reactions, while the NPCR and STNPCR were able to identify the presence of viral DNA in 100% (55/55) of the samples. The high sensitivity combined with the elimination of cross-contamination makes the STNPCR method suitable for the epidemiological studies of PCV2 and can aid in the diagnosis of PMWS.

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“…No Brasil, o PCV2 associado à SMDS foi diagnosticado pela primeira vez em 2000 (ZANELLA & MORES, 2000;CIACCI-ZANELLA & MORES, 2003), e, atualmente, acredita-se que a infecção esteja disseminada no rebanho suíno brasileiro (BARBOSA, 2005). Existem relatos recentes de estudos retrospectivos sobre a identificação da doença: desde 1986, na Espanha (RODRIGUEZ-ARRIOJA et al, 2003), na Inglaterra (SANDVIK et al, 2001) e na Suíça (STAEBLER et al, 2005);desde 1989, no Japão (MORI et al, 2000; e, desde 1993, na Tailândia (KIATPATTANASAKUL-BANLUNARA et al, 2002).…”

Section: Introductionunclassified

Identificação do circovírus suíno tipo 2 por reação em cadeia da polimerase e por imunoistoquímica em tecidos suínos arquivados desde 1988 no Brasil

et al. 2006

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RESUMO A síndrome multissistêmica do definhamento dos suínos (SMDS) é uma doença de importância econômica causada pelo circovírus suíno tipo 2 (PCV2). ABSTRACT Postweaning multisystemic wasting syndrome (PMWS) is of economical importance a disease caused by porcine circovirus type 2 (PCV2). A retrospective investigation was performed on paraffin-embedded organs samples from swine submitted to Embrapa Swine and Poultry Research Center between 1985 and1998

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Perfil sorológico para circovírus suíno tipo 2 em granjas comerciais de suínos no Brasil

Cited by 7 publications

References 12 publications

Serological survey of porcine circovirus 2 antibodies in Northeastern Brazil by immunoperoxidase monolayer assay

Serological survey of porcine circovirus 2 antibodies in Northeastern Brazil by immunoperoxidase monolayer assay

Development and Evaluation of Single-tube Nested PCR (STNPCR) for the Detection of Porcine Circovirus type 2 (PCV2)

Identificação do circovírus suíno tipo 2 por reação em cadeia da polimerase e por imunoistoquímica em tecidos suínos arquivados desde 1988 no Brasil

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