PCR multiplex para identificação de isolados de Clostridium chauvoei e Clostridium septicum

Blackleg is an acute, toxic, infectious, non-contagious disease of domestic and wild ruminants that occurs while the animals are pastured. This article describes an outbreak of blackleg on a farm in Siberia (Russia) in 2019. We provide a detailed description of the cases based on the results of comprehensive diagnostic and epidemiological investigations. For description of case and evaluation, we used the following methods: owner observations, descriptive epidemiology, clinical diagnostics, pathological examination and bacteriology. The distinctive features (in addition to the characteristic features) were as follows: the outbreak of the disease occurred in early spring when there was abundant snow cover and under unfavorable living conditions of animals and traumas; the disease appeared in both vaccinated and unvaccinated cattle; the characteristic clinical signs were low-grade fever, the absence of crepitus, and the presence of haematomas containing erythrocytes with basophilic granularity; thrombs in vessel and vacuolization in tissue of the adrenal gland. This paper aimed to present classical and new clinical and pathology changes in cattle with blackleg in winter conditions of Russian Siberia.

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“…Samples positive for C. chauvoei were analyzed for C. chauvoei and C. septicum using multiplex PCR techniques ( 35 ).…”

Section: Methodsmentioning

confidence: 99%

Blackleg in Cattle in the Irkutsk Region

et al. 2022

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“…Fragments of quadriceps femoris and masseter skeletal muscles were sampled respectively from Calves 1, and 2 from areas of characteristic blackleg lesions. These fragments were immersed in 56°C paraffin to maintain an anaerobic environment [10]; they were then refrigerated at 5°C for 20 days after which were analyzed for C. chauvoei and C. septicum using multiplex PCR techniques [12].…”

Section: Methodsmentioning

confidence: 99%

Blackleg in inadequately immunized calves and their recovery following antibiotic therapy

Guizelini

Silvestre

Ramos

et al. 2020

J Infect Dev Ctries

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Introduction: There is consensus regarding the importance of blackleg vaccination as a preventive measure, and proper immunization protocols are available. However, few studies have evaluated the effectiveness of vaccine protection against Clostridium chauvoei and the treatment of the disease in calves exhibiting early or advanced clinical courses. This study describes twelve blackleg cases in unvaccinated calves and in calves that received a single dose of the vaccine. It also reports the recovery of some calves after antibiotic therapy. Methodology: Two necropsies of cattle dead from blackleg were performed. Fragments of skeletal muscle from these two cattle were immersed in paraffin for multiplex polymerase chain reaction (PCR) analysis. Results: Twelve calves up to nine months of age developed signs of blackleg and eight died. Ten of those 9-month-old calves had received only the first dose of a blackleg vaccine at 4 months of age, but no booster. The last two affected calves belonged to a herd that had never been vaccinated. Four out of five calves treated with penicillin for 6-7 days recovered from the disease. The diagnosis of blackleg was based on necropsy, histopathological findings and detection of C. chauvoei in skeletal muscle samples of two necropsied calves using PCR. Conclusions: The occurrence of cases only in calves that did not receive a booster dose or were not vaccinated indicated that the vaccine used was effective when performed as recommended by the manufacturer. However, neglecting the booster resulted in casualties due to blackleg.

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“…Samples positive for C. chauvoei were analyzed for C. chauvoei and C. septicum using multiplex PCR techniques 34 .…”

Section: The Laboratory Diagnosis Was Based Onmentioning

confidence: 99%

Blackleg in cattle in the Irkutsk region

Blokhin¹,

Toropova²,

Burova³

et al. 2021

Preprint

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Blackleg is an acute, toxic, infectious, non-contagious disease of domestic and wild ruminants that occurs while the animals are pastured. This article describes an outbreak of blackleg on a farm in Siberia (Russia). This article provides a detailed description of these cases based on the results of comprehensive diagnostic and epidemiological investigations. The distinctive features (in addition to the characteristic features) were as follows: the outbreak of the disease occurred in early spring when there was abundant snow cover; the disease appeared in both vaccinated and unvaccinated cattle; the characteristic clinical signs were low-grade fever, the absence of crepitus, and the presence of haematomas containing erythrocytes with basophilic granularity. The evidence suggests that blackleg can present with a variety of conditions and symptoms in cattle.

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PCR multiplex para identificação de isolados de Clostridium chauvoei e Clostridium septicum

Cited by 5 publications

References 6 publications

Blackleg in Cattle in the Irkutsk Region

Blackleg in Cattle in the Irkutsk Region

Blackleg in inadequately immunized calves and their recovery following antibiotic therapy

Blackleg in cattle in the Irkutsk region

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