Experimental infection in Notodiaptomus sp. (Crustacea: Calanoida) with larvae of Camallanus sp. (Nematoda: Camallanidae)

Abstract

This trial registered the experimental infection viability with nematode larvae Camallanus sp. in Notodiaptomus sp., a crustacean, which can be an intermediate host. Adult females of nematode were dissected from the intestines of Xiphophorus maculatus (Osteichthyes: Poeciliidae), at a fish farm in the State of São Paulo. Females were slightly compressed for larvae release, collected with Pasteur pipette and separated on Petri dishes with 9ml filtered water at 28.1ºC, from zooplankton culture. Treatments consisted of Petri dishes with 60 and 105 copepods, in which 120, 150 and 210 larvae of nematode were added in four replications. Twenty-four and 36h after exposition to the larvae, the copepods were fixed in 70% alcohol to record the amount of fixed larvae. Twenty four hours after exposition, 60 copepods group with 120 larvae showed significantly higher prevalence (46.5%) when compared to 105 copepods and 120 larvae (33.2%). Thus, these answers suggested that 120 larvae were enough for a successful infectivity. Experimental infection was available and so, it was used as a pattern to life cycle studies of camallanid nematodes and hosts susceptibility tests.

Controle alternativo de helmintos de Astyanax cf. zonatus utilizando fitoterapia com sementes de abóbora (Cucurbita maxima) e mamão (Carica papaya)

“…Além disso, os nematóides necessitam de um hospedeiro intermediário para completar o ciclo de vida, dessa forma, a manutenção dos peixes em condições isentas de hospedeiros intermediários (zooplâncton) e a alimentação com a ração comercial pode-se ter quebrado esse ciclo impedindo novas reinfestações. Segundo Martins et al (2007), a presença de microcrustáceos como primeiro hospedeiro intermediário do nematóide do gênero Camallanus sp. causam impactos negativos nos cultivos de peixes devido a essa parasitose.…”

Parasitic fauna of eight species of ornamental freshwater fish species from the middle Negro River in the Brazilian Amazon Region

“…Some of them may cause host death because of the way in which they become attached to the intestinal epithelium (FERRAZ, 1999;THATCHER, 2006). Camallanid nematode species generally use a copepod as an intermediate host, as noted by Moravec, Wolter e Korting (1999) and demonstrated by Martins et al (2007) in a study on experimental infection of Notodiaptomus sp. with Camallanus maculatus.…”

“…After sacrifice, the fish were necropsied for parasitological examination (GHIRALDELLI et al, 2007). The parasites were processed (EIRAS; TAKEMOTO; PAVANELLI, 2000;MARTINS, 2007) and counted (TAVARES-DIAS; MARTINS; MORAES, 2001a). They were identified in accordance with Thatcher (2006), and the prevalence and mean intensity were calculated in accordance with Bush et al (1997).…”

Seasonality of nematode larvae in Iguanodectes spilurus (Characidae) an ornamental fish from northeastern Para, Brazil

“…The occurrence of Procamallanus L 3 larvae in I. spilurus suggests that the feeding habitat of this fish may be composed of copepods, as verified by Martins et al (2007) in experimental infection with camallanid larvae in Notodiaptomus sp. (Crustacea: Calanoida).…”