Influência de Agaricus blazei Murrill sobre o tumor sólido de Ehrlich e linfonodos poplíteos de camundongos

Abstract

RESUMOEstudou-se a influência do tratamento diário com filtrado aquoso de Agaricus blazei Murrill (ABM) (25mg/ml), via oral, por 17 e 57 dias, em camundongos inoculados com tumor sólido de Ehrlich (TSE) por meio da curva de crescimento tumoral, do peso relativo do tumor, da contagem de regiões organizadoras de nucléolos (AgNORs) e dos padrões histológicos das massas tumorais e linfonodos poplíteos. Os animais que ingeriram o extrato aquoso do ABM por 57 dias apresentaram menor (P<0,05) crescimento do TSE, no segundo e terceiro dias, fase inflamatória do crescimento tumoral. Quanto ao peso relativo das massas tumorais, valores de AgNORs, padrões morfoistopatológicos do TSE e ocorrência de processos metastáticos, não houve alteração significativa (P>0,05) entre os animais tratados e não tratados.Palavras-chave: camundongo, Agaricus blazei, tumor de Ehrlich, histopatologia ABSTRACTThe influence of daily administration of Agaricus blazei Murrill (ABM) aqueous solution (25mg/ml) during 17 and 57 days in mice bearing solid Ehrlich tumor was studied. Tumoral growth, tumoral and spleenic relative weights, nucleoly organization regions AgNORs values and tumor and popliteal lymph nodes histopathology were daily evaluated. The animals that received ABM during 57 days showed lower values (P<0.05) of tumoral growth on second and third days after the period corresponding to the inflammatory phase of tumoral growth. The relative weight of the tumor and AgNORs values were similar (P>0.05) between treated and non-treated animals. No difference in microscopic evaluation of the tumors in treated and non-treated animals was seen and metastasis in popliteal nodes of the tumor occurred in all the animals.

Molecular changes associated with the anticancer effect of sulforaphane against Ehrlich solid tumour in mice

“…In several previous reports, Ehrlich (murine mammary adenocarcinoma) solid tumour has been selected as the carcinogenesis experimental model. [ 13–15 ] This tumour is easily developed and transferred in vivo and can be utilised for the analysis of carcinogenic processes and to determine the impact of novel treatment approaches on tumours.…”

Experimental orthotopic breast cancer as a model for investigation of mechanisms in malignancy and metastasis to the lymph nodes

“…The Ehrlich tumor resembles human tumors; its neoplastic cells are undifferentiated and have a fast growth rate, leading to an increase in chemotherapy sensitivity in mice [19]. This tumor has been used as an experimental model for several different investigations, including antitumor effi ciency of photodynamic therapy and magnetohyperthermia [3,[21][22][23] pharmacokinetic studies of chemotherapeutics [24,25] evaluation of tumor growth under effects of Agaricus blazei, plant extracts and antioxidants [26]. However, most studies have been conducted using ectopic transplantation into the peritoneal cavity in order to acquire the ascitic tumor, or into the head, femoral region, tongue and footpad, in order to obtain solid tumors [3,[21][22][23][24][25][26][27][28].…”