The protective effects of nutritional antioxidant therapy on Ehrlich solid tumor-bearing mice depend on the type of antioxidant therapy chosen: histology, genotoxicity and hematology evaluations

Comparative Efficacy of a Biocompatible Citrate-Functionalized Magnetic Fluid Mediating Radiofrequency Hyperthermia and Magnetohyperthermia to Treat Ectopic Ehrlich-Solid-Tumor-Bearing Elderly Mice

“…Significant alterations of increased lymphocytes were observed, simultaneously with decreased monocyte frequency, when the peritoneum was exposed for 10 minutes, not observing alterations in the neutrophil frequency at any time after AC field exposure, an important signature of inflammatory reaction [28]. Although these results appear contradictory in respect to ours, this study was carried out with healthy animals [28], and the installation of the Ehrlich solid tumor by itself has been demonstrated by our group as non-significantly decreasing lymphocytes and significantly increasing neutrophils+monocytes [32], corroborating our results. Even if the 10 minutes of magnetic field exposure and treatments used were previously tested [15,19,28,35] and have been chosen to be sufficient to induce hyperthermia with practically no hematological adverse effects, we opted in this present work to increase the exposure time to CMagMHG by three minutes (maximum time that the animals were anesthetized/asleep during exposure), in order to compare with the Magnetherm equipment, where the animals remain dormant until the end of the anesthesia.…”

“…Ehrlich tumor: The Ehrlich ascitic tumor was maintained in ascitic form by passages in Swiss mice, by weekly intraperitoneal transplantation of 10 6 tumor cells [32]. The ascitic fluid, collected by intraperitoneal puncture, was counted in a Neubauer hemocytometer, and the Trypan blue dye exclusion method was used to evaluate the viable cells [33].…”

“…For this, the sample was dehydrated in a low vacuum environment and stored as a powder without atmospheric contact; using a Zetasizer (Zetasizer Nano-ZS90 Malvern Instruments Limited, Malvern, UK) we determined the hydrodynamic radius, the polydispersion index and its surface charge by dynamic light scattering (DLS) with the sample diluted in ultra-pure deionized water (1: 100), and the measurements were performed in triplicate at 25°C, with a fixed detection angle of 173°C; the physical mean diameter and its dispersion was determined by transmission electron microscopy (TEM) performed on a JEOL 1011 (JEOL USA, Inc.) with sample diluted in ultra-pure deionized water (1:500), deposited in a 300 mesh copper TEM grid supported by films of Formvar, dried at room temperature for 24 hours and then counterstained for 45 minutes with 1% Osmium (OsO 4 ) vapor. The average diameter of the sample evaluated by MET implantation [15,32,35,36]. Following 48 hours after xenograft, all mice had clinical tumor and received the following treatments: (a) filtered water and non-tumor-bearing mice (negative control); (b) xenograft and no treatment (tumor control); (c) intratumoral injection of NPCit (40 µL) containing 18 × 10 18 particles/mL and exposure for 13 minutes to CMagMHG; (d) intratumoral injection of NPCit and exposure for 13 minutes to Magnetherm.…”

Bioactive compounds and functional potential of pequi (<em>Caryocar</em> spp.), a native Brazilian fruit: a review

“…Many cancer-chemopreventive agents possess antioxidant potential, and biological antioxidants contain bioactive phytochemicals that may play a vital role in protecting cells from oxidative stress (Miranda-Vilela et al, 2011). Animal studies have demonstrated that the administration of pequi could improve the antioxidant system and consequently decrease the advance of carcinogenesis (Miranda-Vilela et al, 2014;Almeida et al, 2012;Miranda-Vilela et al, 2011).…”

Preparation, Biodistribution of 99mTc-Azacytidine Complex as a Potential Agent for Radiotherapy

“…Ehrlich carcinoma is the correspondent of murine mammary adenocarcinoma. It has been chosen as a tumour model for this biodistribution study due to its ease of transplantation, aggressiveness and rapid growth 28,29 . As mentioned earlier, the purpose of the radiolabeling of 5-Aza with 99m Tc was to study the possibility of its future radiolabeling with 188/186 Re for radiotherapeutic use.…”