Dairy cow abortion associated with Neospora caninum and other infectious agents

Santos, Ana Paula Millet Evangelista dos; Navarro, Italmar Teodorico; Bracarense, Ana Paula Frederico Rodrigues Loureiro; Freire, Roberta Lemos; Marana, Elizabete Regina Marangoni; Ogawa, Liza; Alfieri, Amauri Alcindo; Freitas, Júlio César de; Vidotto, Odilon

doi:10.1590/s0102-09352005000400017

Cited by 10 publications

(9 citation statements)

References 4 publications

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“…Several other studies have shown frequencies similar to the ones in the present study but with different cutoffs. Gondim et al (1999), Costa et al (2001), Ogawa et al (2005) and Santos et al (2005) detected frequencies of 14,09%, 16,83%, 12,00%, and 14,30% with a cutoff of 1:200, respectively, in dairy cattle in Bahia, cows for slaughter from the states of São Paulo and Minas Gerais, dairy cattle from northern Paraná, and dairy cows with aborted calves in the north of Paraná. Aguiar et al (2005) obtained the following frequencies at a 1:25 cutoff: 11,20% for dairy cattle, 9,50% for beef cattle, and 9,70% for dual purpose cattle reared in the city of Monte Negro, Rondônia.…”

Section: Resultsmentioning

confidence: 96%

Serological survey of Neospora caninum in dairy herds from Parauapebas, State of Pará

et al. 2015

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Neospora caninum is a coccidian protozoan that affects cattle worldwide causing economic losses. To survey the frequency of anti-N. caninum antibodies in dairy herds in the municipality of Parauapebas, Southeast of Pará, samples of 465 sera from 45 farms were subjected to indirect immunofluorescence assay (cutoff 1:100). Anti-N. caninum antibodies were found with a frequency of 13,33% with the following titer distribution: 27 (43,55%) for titer 100; 14 (22,58%) for 200; 16 (25,80%) for 400, and 5 (8,07%) for 800; no serum titers more than 800 was noted, but at least one positive animal was present in most farms. The highest frequency occurred among females, with no significant difference in frequency between the sexes (Fisher exact test = 0,59, P = 0,99); 2-year-old animals had the highest frequency, although their numbers were lesser than those aged >5 years, with no significant difference in frequency between the age groups (χ 2 = 2,1, P = 0,71). No significant difference was observed between the frequency and occurrence of abortion (χ 2 = 2,3, P = 0,13) and frequency and presence of dogs in the farms (χ 2 = 0,26, P = 0,60). Actions toward health monitoring are recommended to prevent the entry of new sources of N. caninum and to control its spread within herds.

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Section: Resultsmentioning

confidence: 96%

Serological survey of Neospora caninum in dairy herds from Parauapebas, State of Pará

et al. 2015

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“…This study demonstrated that 26.7% (8/30) of the serum evaluated fetuses were positives to N. caninum antibodies; this results is more elevated, despite the use of different techniques, than that described in a similar study (SANTOS et al, 2005a), that used the IFA and obtained 11.7% (4/34) of seropositivity.…”

Section: Discussionmentioning

confidence: 40%

Occurrence of abortions induced by Neospora caninum in dairy cattle from Santa Catarina, southern Brazil

Macedo

Miura

et al. 2017

Rev. Bras. Parasitol. Vet.

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The aim of the present study was to investigate the occurrence of N. caninum associated with abortions of dairy cattle from Santa Catarina state, southern Brazil by using enzyme-linked immunosorbent assay (ELISA), immunohistochemistry (IHC), and polymerase chain reaction (PCR). Blood from dairy cows that aborted along with intrathoracic fluid and tissue samples (brain, heart, liver, and lung) from their fetuses were collected and used for serology; PCR, histopathological, and immunohistochemistry (IHC) evaluations were also conducted. Twenty-one cows (51.2%) out of 41, and eight fetuses (26.7%) out of 30 were ELISA (HerdCheck, IDEXX) positive for N. caninum. Dams > 36 months of age had a higher risk of being serum positive than younger animals. PCR and IHC revealed that 38.8% (14/36) and 25.0% (9/36) of the fetuses were positive for N. caninum, respectively for each of the tests. Seropositive cows had a higher frequency of fetuses that were also positive by either intrathoracic fluid, PCR, or IHC. In summary, the present study observed a high frequency of N. caninum in abortions from dairy cows from southern Brazil, with a higher N. caninum prevalence found in cows that were older than 36 months. In addition, serology, PCR, and IHC should be used all together for better diagnosis of neosporosis in cattle.Keywords: Neosporosis, fetuses, lesion, serology, cattle. ResumoO objetivo deste estudo foi avaliar a ocorrência de N. caninum associado a abortamentos em vacas de leite do estado de Santa Catarina, sul do Brasil pelo uso das técnicas de ELISA (HerdCheck, IDEXX), reação em cadeia pela polimerase (PCR) e imunohistoquímica (IHC). O sangue das vacas leiteiras que abortaram, bem como, o líquido intratorácico e amostras de tecidos (cérebro, coração, fígado e pulmão) de seus fetos foram coletados e usados para sorologia, PCR (Np21+ e Np6+), e IHC. Vinte e uma vacas (51,2%) de um total de 41, bem como, oito fetos (26,7%) de um total de 30 foram positivos no ELISA (IDEXX) para N. caninum. As vacas > 36 meses de idade tiveram um maior risco de serem soropositivas do que os animais mais jovens. PCR e IHC revelaram que 38,8% e 25,0% dos fetos foram positivos para N. caninum, respectivamente para cada um dos testes. As vacas soropositivas tiveram uma maior frequência de fetos que também foram positivos no fluído intratorácico, na PCR ou na IHC. Em resumo, o presente estudo observou uma alta frequência de N. caninum em abortos de vacas leiteiras na região estudada, com maior prevalência de N. caninum em vacas com mais de 36 meses de idade. Além disso, sorologia, PCR e IHC deveriam ser utilizadas conjuntamente para melhor diagnóstico de neosporose em bovinos.

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“…The results found that brucellosis were according to the study conducted with dairy cattle in Parana by Santos et al (2005). This suggests that the mandatory measures required by the National Program for Control and Eradication of Brucellosis and Tuberculosis (PNCEBT) implemented in 2006, is effective.…”

Section: Discussionmentioning

confidence: 91%

“…Despite the seropositive samples in a large number of the animals of the herd, a study in Canada carried out by Waldner (2005) stated that the use of large-scale diagnostic tests is questionable because of the high cost. Santos et al (2005) studied the prevalence of brucellosis, leptospirosis and neosporosis in animals with a history of miscarriage in Paraná, and found that 60% (21/35) were reactive to one of the diseases. From the 21 positive animals, 14.3% were for neosporosis, 42% for leptospirosis and no animals were reactive to brucellosis, resembling the results obtained herein.…”

Section: Discussionmentioning

confidence: 99%

“…From the 21 positive animals, 14.3% were for neosporosis, 42% for leptospirosis and no animals were reactive to brucellosis, resembling the results obtained herein. For the seropositivity diagnosis, Santos et al (2005) considered titers higher than or equal to 1:200, similar to the one as used in this study. Langoni et al (2013) reported that the titration used is one of the important factors when estimating the prevalence of Neospora caninum, along with other factors such as race and test used.…”

Section: Discussionmentioning

confidence: 99%

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Occurrence of brucellosis, leptospirosis and neosporosis in cows with retained placenta in Southwest Paraná, Brazil

et al. 2018

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The aim of this study was to measure the occurrence of brucellosis, leptospirosis and neosporosis in cows from 25 family farms milk, with semi-intensive grazing system, located in the Southwest region of Paraná, Brazil. Eighty-four cows with retained placenta, between July 2013 to July 2014, diagnosed by the presence of fetal membranes in the uterus up to 12 hours after partum, decrease of appetite and of milk production, as well increase of rectal temperature were included into the study. The animals were submitted to blood collection for evaluating the seropositivity for brucellosis, leptospirosis and neosporosis. The blood was collected at 15 to 45 days postpartum, avoiding false negative results due to immune deficiency observed in the transition period. After collection, the blood was centrifuged, the serum was packed in three aliquots, identified and subsequently frozen. Serology was performed to diagnose brucellosis (technique of buffered acidified antigen), leptospirosis (through the microscopic agglutination test - MAT) and neosporosis (indirect immunofluorescence test). Animals were considered positive when presenting titer >1:100 (leptospirosis) and 1:200 (neosporosis). No animal with placenta retention presented Brucella abortus bacteria; 39% (33/84) were reactive to one or more Leptospira serovars, 15% (13/84) were positive to Neospora caninum, and 4% (4/84) had both diagnosis, being reactive for leptospirosis and neosporosis. In conclusion, data from this experiment inspires greater attention to leptospirosis and neosporosis in dairy cattle presenting placenta retention in southwest Paraná.

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Dairy cow abortion associated with Neospora caninum and other infectious agents

Abstract: Dairy cow abortion associated with Neospora caninum and other infectious agents[Aborto em vacas leiteiras associado a Neospora caninum e a outros agentes infecciosos]

Cited by 10 publications

References 4 publications

Serological survey of Neospora caninum in dairy herds from Parauapebas, State of Pará

Serological survey of Neospora caninum in dairy herds from Parauapebas, State of Pará

Occurrence of abortions induced by Neospora caninum in dairy cattle from Santa Catarina, southern Brazil

Occurrence of brucellosis, leptospirosis and neosporosis in cows with retained placenta in Southwest Paraná, Brazil

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