Serum and urinary measurements of prostatic acid phosphatase (PAP) and prostatic specific antigen (PSA) in dogs

Abstract

Serum and urinary prostatic acid phosphatase (PAP) and prostatic specific antigen (PSA) from 20 dogs were measured. PAP and PSA tests were carried out in authomatized equipment with commercial kits used for humans. Mean PAP serum value was 0.7U/l and urinary 0.1U/l. Mean serum and urinary PSA were 0.005ng/dl and 0.004ng/dl, respectively. In vivo determination of these two biomarkers in dogs is a new form of diagnosis in veterinary medicine and these values should be correlated with the morphological lesion of the prostate gland.Keywords: dog, prostatic acid phosphatase, PAP, prostatic specific antigen, PSA RESUMO Realizaram

Prostatic acid phosphatase in serum and semen of dogs

“…The measurement of this enzyme in the prostatic fluid is important, because variations due to alterations in the gland can be easily detected, since this material has more PAP. Presence of PAP was observed in canine urine (Amorim et al 2004, Moura et al 2006, but the levels were lower than those observed for seminal plasma in that study. There was a variation in the serum and seminal PAP values within each studied in the present study, endorsing a previous report in which values of PAP found in the serum of 90 human patients with BPH ranging from 0.24 to 20.8 ng/mL, while in 39 patients with prostatic carcinoma these values ranged between 0.6 -150 ng/mL (Alivizatos et al 1992).…”

“…The PAP values in the serum were lower than those obtained in some other studies (Corazza et al 1994, Souza 1998, Amorim et al 2004, Moura et al 2006. This could be explained by the difference in the reagent used by those authors, which contained tartarate, a PAP inhibitor, and the reagent used in this study, phosphate timolftaleine, a substrate for PAP.…”

“…However, it is similar to Reiman (2005) results that found no correlation between serum PAP concentrations and age or body weight. Amorim et al (2004) evaluated PAP concentrations in serum and urine of middle age (4-6 years old) and old dogs (7-11 years old) and observed difference only in urinary concentrations between the two groups. The values of this enzyme pre- Table 2.…”

Punção aspirativa com agulha fina guiada por ultrassom e biópsia por videolaparoscopia em próstata de cães

“…Os exames histológicos forneceram informações sobre a estrutura e a morfologia do parênquima prostático, tornando possível a caracterização morfológica das células e a diferenciação dos processos proliferativos de natureza benigna ou maligna como descrito por Amorim et al (2004). Um dos animais apresentou alterações na estrutura do parênquima da glândula, compatíveis com hiperplasia prostática benigna, como aumento do tamanho dos ácinos glandulares e aumento do número das camadas celulares ao redor de cada ácino.…”

“…Exames radiográficos, ultrassonográficos, microbiológicos do sêmen, citológicos e histológicos da próstata contribuem para a diferenciação das afecções da glândula. Também as dosagens da fosfatase ácida prostática (PAP) e do antígeno prostático específico (PSA) são importantes para esta diferenciação (Hedlund, 2002;Amorim et al, 2004). A ultrassonografia é um procedimento seguro, não invasivo, que pode ser utilizada não somente para avaliar o tamanho e o contorno da próstata, mas também para avaliar o parênquima glandular e as estruturas com fluido em seu interior (Mattoon e Nyland 1995;Peter et al, 1995;Souza e Toniollo, 1999;.…”