Achados histológicos em fetos bovinos abortados e não abortados

Okano, Werner; Bracarense, Ana Paula Frederico Rodrigues Loureiro; Reis, Antônio Carlos Faria dos; Alfieri, Amauri Alcindo

doi:10.1590/s0102-09352003000200016

Cited by 2 publications

(4 citation statements)

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“…However, moderate to severe degeneration(s) can be interpreted as abnormal findings. Okano et al (2003) attributed hepatic vacuolization to the accumulation of glycogen in aborted animals, confirmed by PAS staining. Although hepatic glycogen was found in some animals, which is considered to be normal in mild to moderate amounts (Thomson 1998), a pathological finding (i.e., significant amount) was present in 8 of 41 cloned and 1 of 4 control calves.…”

Section: Discussionmentioning

confidence: 84%

“…These changes were prominent in clones of advanced age, which may indicate liver regeneration in response to enteral feeding and independence from placental nutrition. The presence of hepatocyte vacuolization in the liver of bovine fetuses (Okano et al 2003) and bovine neonates from in vitro fertilization (Prestes et al 2009) has also been reported. It can be inferred, therefore, that some degree of hepatocyte vacuolization is normal in newborn animals.…”

Section: Discussionmentioning

confidence: 89%

“…It was not possible to accurately identify this pigmentation; however, it is known that the observed pigment does not originate from the accumulation of bile pigments, iron, hemosiderin or glycogen, as no characteristic coloring was evident with the techniques used (i.e., Hall, Perls, and PAS, respectively). Okano et al (2003) described vacuolization of the renal tubular epithelium in aborted calves that exhibited positive staining using the PAS method. In the present study, PAS staining did not characterize the granules observed in the tubular epithelium.…”

Section: Discussionmentioning

confidence: 99%

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Pathological features of cloned calves that died in the neonatal period

et al. 2020

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Somatic-cell nuclear transfer is a cloning technique that enables the creation of a viable embryo from a donor adult to produce a genetically identical individual. This technique opens numerous potential possibilities for medicine and animal reproduction. However, several reports have documented cloning-related issues. Embryo and fetal losses remain significantly higher than in other techniques, and there is a high incidence of dystocia and hydrops, which decreases efficiency and increases costs. Animals delivered at term often exhibit a syndrome known as macrosomia and experience difficulties in adapting to life outside the uterus, and death is a common outcome. In the present study, 41 cloned calves that died in the neonatal period were subjected to gross and histopathological examination. Most important gross lesions were found in the liver (enlargement, congestion, yellowish color), kidneys (brownish color at surface and cut, and cysts), lungs (atelectasis, parenchymal consolidation, and secretions in bronchi and bronchioles), and heart (concentric and eccentric hypertrophy, hematic cysts, persistence of ductus arteriosus). Primary microscopic findings were seen in the liver, kidneys, and lungs from neonatal calves. In the liver, 85% of the animals exhibited hepatic degeneration. The presence of a brownish pigment within the cortical tubules of the kidneys was found in approximately 90% of the samples; the presence of this pigment has not been previously reported in cloned calves. In the lungs, a large number of animals exhibiting lesions characteristic of pneumonia (55%). These changes were the pivotal causes of death, mainly due to problems in adapting to life outside the uterus and opportunistic infections in the neonatal period. Further investigation focusing on pathological anatomical changes is necessary to map these abnormalities in cloned animals.

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Section: Discussionmentioning

confidence: 84%

Section: Discussionmentioning

confidence: 89%

Section: Discussionmentioning

confidence: 99%

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Pathological features of cloned calves that died in the neonatal period

et al. 2020

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“…In cattle, several factors can interfere in reproductive performance, among which are those related to the pathophysiology of the reproductive system, genetics, nutrition and health management of livestock herds (Corrêa et al 2000, Royal and Flint 2000, Sayers et al 2015. Herd health, in particular, infections that directly or indirectly compromise the reproductive tract of the female and male and the viability of the embryo and fetus, are the main factors affecting reproductive efficiency of cattle (Vanroose and Van 2000, Okano et al 2003, Takiuchi et al 2005.…”

Section: Introductionmentioning

confidence: 99%

Immune-serological identification of infectious agents with influence on bovine embryo transfer in the north of Brazil

et al. 2016

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ABSTRACT. The aim of this study was to investigate antibodies against brucellosis, leptospirosis, bovine rhinotracheitis (IBR/ BoHV-1), bovine viral diarrhea (BVD) and neosporosis through immunodiagnostic. These agents may directly or indirectly compromise the female bovine reproductive tract as well as embryonic and fetal development, therefore we also tried to establish a probable relation between those antibody with the utilization rates of fixed-time embryo transfer (FTET) protocols, with pregnancy rate per embryo transfer (ET) and the occurrence of miscarriages. Embryos were obtained by in vitro fertilization of the ovum and sperm from the Gyr dairy breed, which were transferred to 235 zebu-crossbred cows under FTET protocols. The blood samples to obtain serum were collected by venous puncture from coccygeal vein, only a few days of the completion of ET. For the immunodiagnostic research of brucellosis, leptospirosis, IBR/BoHV-1, BVD and neosporosis, the complement fixation assay, the microscopic agglutination test, virus neutralization, and linked immunosorbent assay to enzymes in conjunction with the indirect immunofluorescence were used. The utilization rates of the FTET protocols related to pregnancy and abortion in D16, D35, and D75 were 67.23%, 34,18% and 20,37% respectively. The immunodiagnostic results related to seropositive animals were obtained for brucellosis (7/235 = 2.89%), leptospirosis (128/235 = 54.47%), IBR (103/235 = 43.83%), BVD (174/235 = 74.04%), and neosporosis (150/235 = 63.82%). In seropositive recipients, there was an observed influence (P < 0.05) of leptospirosis and neosporosis on the rate of protocol utilization of brucellosis on both the pregnancy and abortion rate, and of BVD and IBR on abortion rate.Key words: reproductive, embryo, recipients, cattle. RESUMEN.El estudio tuvo por finalidad evidenciar, mediante técnicas de inmunodiagnóstico, anticuerpos contra Brucelosis, Leptospirosis, Rinotraqueítis Infecciosa Bovina (IBR), Diarrea Viral Bovina (DVB) y Neosporosis, agentes que directa o indirectamente pueden comprometer el tracto reproductor de las hembras así como el desarrollo embrionario y fetal. Se estableció una probable relación entre la presencia de anticuerpos contra los agentes con la tasa de aprovechamiento de protocolos de transferencias de embriones en tiempo fijo (TETF), la tasa de preñez por transferencia de embriones (TE) y la ocurrencia de abortos. Los embriones fueron obtenidos por fertilización in vitro (FIV) a partir de aspiración folicular y semen de toros, ambos de raza Gyr lechero, y tranferidos a 235 vacas receptoras cruzadas con Cebú sometidas a protocolos para TETF. Se obtuvo suero de muestras de sangre mediante venopunción de la vena coccígea solamente en los días de realización de TE. Para evidenciar Brucelosis, Leptospirosis, IBR, DVB y Neospora se emplearon las técnicas de fijación de complemento, seroaglutinación microscópica, seroneutralización y test de inmunoensayo (ELISA) junto con la inmunofluorescencia indirecta (RIFI), respectivamente. El ...

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Achados histológicos em fetos bovinos abortados e não abortados

Cited by 2 publications

References 3 publications

Pathological features of cloned calves that died in the neonatal period

Pathological features of cloned calves that died in the neonatal period

Immune-serological identification of infectious agents with influence on bovine embryo transfer in the north of Brazil

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