Quantificação das regiões organizadoras de nucléolo (NORs) como parâmetro para avaliar a proliferação das células da granulosa

Silva, C.M.; Serákides, Rogéria; Nascimento, Evaldo; Nunes, V.A.; Ribeiro, A.F.C.; Ocarino, Natália de Melo

doi:10.1590/s0102-09352003000100019

Cited by 5 publications

(3 citation statements)

References 13 publications

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“…In a study of granulosa cells of secondary follicles in rats, Silva et al (2003) observed that in the secondary follicles with two to three layers of granulosa cells, the NORs were large and present in low numbers. In the more developed secondary follicles with greater cell proliferation, the granulosa cells presented generally small NORs, dispersed throughout the nucleus and in significantly higher numbers, thus demonstrating that NORs with larger areas and low quantities indicate less cellular proliferation, while smaller NORs in greater quantities indicate increased cell proliferation.…”

Section: Resultsmentioning

confidence: 99%

Evaluation of cell proliferation and endometrial thickness of bitches in different periods of diestrus

Gossler

Santos

Azevedo

et al. 2017

An. Acad. Bras. Ciênc.

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This study aimed to determine the histological features of the endometrium of bitches, as well as the cell proliferation at specific moments of diestrus, 10, 20, 30, 40, 50 and 60 days post ovulation, correlating the endometrial thickness with the uterine cell proliferation and the metabolic state (weight, blood glucose and plasma cholesterol) of the animals. Therefore, the right and left uterine horns of 26 clinically healthy bitches submitted to ovariohysterectomy were histologically analyzed 10, 20, 30, 40, 50 and 60 days post ovulation. The hematoxylin-eosin and AgNOR staining techniques were performed. All parameters were evaluated by ANOVA and post-hoc Tukey test (p<0.05). The correlation between endometrial thickness and uterine cell proliferation, weight, blood glucose and plasma cholesterol of animals was observed using the Pearson method (p<0.05). In the present study, it is concluded that endometrial thickness does not differ at any of the moments analyzed in diestrus. The endometrial thickness is not influenced by hormones, weight, blood glucose or serum cholesterol of bitches in this phase of the estrous cycle. However, there is greater cell proliferation in the endometrium at day 40 compared to day 60 post ovulation under the influence of the endocrine profile.

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Section: Resultsmentioning

confidence: 99%

Evaluation of cell proliferation and endometrial thickness of bitches in different periods of diestrus

Gossler

Santos

Azevedo

et al. 2017

An. Acad. Bras. Ciênc.

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“…2), o que aponta a participação dos hormônios tireoidianos no desenvolvimento folicular. Resultados semelhantes também já foram observados em fêmeas pré-púberes (Chan e Ng, 1995;Dijkstra et al, 1996 As células da granulosa de folículos secundários com duas a três camadas de células da granulosa apresentam menor número de AgNORs quando comparadas às de folículos secundários com mais de cinco camadas de células, confirmando que essa técnica é boa para diferenciar o potencial proliferativo dessas células também em processos não neoplásicos (Silva et al, 2003). Além disso, resultados de pesquisas comprovaram correlação positiva entre o número de AgNORs e outros parâmetros da cinética celular que já estão bem estabelecidos como detectores da atividade proliferativa das células, tais como o MIB-1, o Ki-67, o PCNA e a incorporação de bromodeoxiuridina (BrdU) (Derenzini, 2000).…”

Section: Resultsunclassified

Histomorfometria e histoquímica dos ovários, tubas e útero de ratas hipotireóideas em metaestro-diestro

Silva

Serákides

Oliveira

et al. 2004

Arq. Bras. Med. Vet. Zootec.

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RESUMOA foliculogênese ovariana foi estudada em ratas adultas Wistar, hipotireóideas na fase de metaestrodiestro. O hipotireoidismo foi induzido pela administração oral e diária de propiltiouracil (1mg/animal). As ratas eutireóideas receberam placebo. Após 120 dias de tratamento, foi colhido o plasma para dosagem de tiroxina livre, progesterona e estradiol após o que foram sacrificadas para colheita dos ovários, tubas e útero, para avaliação histomorfométrica e histoquímica. O hipotireoidismo reduziu significativamente o peso dos ovários e o número de folículos secundários e terciários e de corpos lúteos sem, no entanto, alterar a porcentagem de folículos atrésicos e o número de folículos primários e pré-ovulatórios. As células da granulosa dos folículos secundários das ratas hipotireóideas apresentavam núcleo pequeno com significativa redução do número de regiões organizadoras de nucléolo (NORs). Essas mudanças não alteraram os valores periféricos de estradiol e de progesterona. Houve redução significativa da espessura do endométrio, do número de glândulas endometriais e da altura do epitélio do infundíbulo.Palavras-chave: rata, hipotireoidismo, ovário, tuba, útero, NORs ABSTRACT Ovarian folicullogenesis was studied in adult

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“…This silver staining allows a quantitative analysis of NORs, which are related to protein synthesis, being increased in number according to cell activity. This tool has been used to detect proliferation of neoplastic cells (Gupta et al., ); however, it has also been validated to assess the proliferative potential of granulosa cells of non‐neoplastic ovarian follicles (Silva et al., ).…”

Section: Introductionmentioning

confidence: 99%

Different associations of cryoprotectants for testicular tissue of prepubertal cats submitted to vitrification

Lima

Silva

Morais

et al. 2016

Reprod Domestic Animals

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The cryopreservation of testicular tissue is presented as the only alternative for the preservation of genetic material from prepubertal animals. However, this biotechnology is still being tested. The objective of this study was to evaluate the effect of different associations of cryoprotectants and the potential of cell proliferation after vitrification of testicular tissue of prepubertal cats. Five testicular pairs from five prepubertal cats were used, and each pair was divided into four fragments. Of these, one fragment composed of the control group (CG) and the rest were distributed in experimental groups according to the associations of cryoprotectants to be tested (dimethyl sulphoxide (DMSO)/glycerol (GLY); ethylene glycol (EG)/GLY) or DMSO/EG) in a final cryoprotectant concentration of 5.6 m. The fragments were submitted to vitrification, and after one week, fragments were heated and processed for histomorphological evaluation and quantification of nucleolar organizer regions (NORs). DMSO/GLY did not differ from CG and was superior to the other vitrified groups, as to cell separation and degree of shrinkage of the basal membrane. Concerning cell differentiation, visibility of the nucleus and nuclear condensation, all the vitrified groups were inferior to CG; however, DMSO/EG was inferior to DMSO/GLY and EG/GLY, which did not differ among themselves. CG was superior to all groups in quantification of NORs. DMSO/EG was inferior to all others, and there was no difference between DMSO/GLY and EG/GLY. The association DMSO/GLY presented the best preservation of tissue integrity and potential of cell proliferation after vitrification of the testicular tissue of prepubertal cats.

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Quantificação das regiões organizadoras de nucléolo (NORs) como parâmetro para avaliar a proliferação das células da granulosa

Cited by 5 publications

References 13 publications

Evaluation of cell proliferation and endometrial thickness of bitches in different periods of diestrus

Evaluation of cell proliferation and endometrial thickness of bitches in different periods of diestrus

Histomorfometria e histoquímica dos ovários, tubas e útero de ratas hipotireóideas em metaestro-diestro

Different associations of cryoprotectants for testicular tissue of prepubertal cats submitted to vitrification

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