Qualidade espermática do sêmen criopreservado de cães: I - efeito do meio diluidor

We evaluated the capacity of ocelot and oncilla spermatozoa to bind to the perivitelline membranes (PVMs) of hen eggs in a sperm binding assay (S-PVM). In addition, a device that improves the standardization of the assay was developed. The number of sperm bound to the PVM in fresh (T1) and frozen-thawed (T2) semen from both species was compared to the sperm quality observed in routine tests. The PVM was stretched on a circular silicone device to create a standardized area for analysis. In both treatments and for both species, the spermatozoa were able to bind to the PVM, indicating that PVM may be used for a sperm binding assay in ocelot and oncilla. The S-PVM assay did not differ in fresh and frozen-thawed ocelot sperm (p>0.05). However, fewer oncilla sperm (p<0.05) were bound to the PVM in T2, indicating that the proposed test may be able to detect injuries that compromise sperm binding abilities. The device maintained the PVM stretched during the processing and defined the evaluation area.

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Ocelot and oncilla spermatozoa can bind hen egg perivitelline membranes

Araújo

Paula

Deco‐Souza

et al. 2015

Animal Reproduction Science

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Comparação entre duas concentrações de glicerol para a criopreservação de sêmen de suçuarana (Puma concolor)

et al. 2013

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Pesq. Vet. Bras. 33(4):512-516, abril 2013 512 RESUMO.-O desenvolvimento de biotécnicas de reprodução é uma importante ferramenta para a conservação de animais silvestres ameaçados de extinção. Procedimentos de reprodução assistida em suçuarana, no entanto, são escassos na literatura, em especial aqueles relacionados à criopreservação de sêmen. Neste sentido, o presente trabalho objetivou avaliar a congelabilidade do sêmen de suçuaranas adultas mantidas em cativeiro, por meio da comparação entre duas The development of biotechnologies of reproduction is an important tool for the conservation of wild animals threatened with extinction. Assisted reproduction procedures in mountain lions, however, are scarce, especially those related to sperm cryopreservation. In this context, this study aimed to evaluate the freezing capacity of semen from adult mountain lions in captivity through the comparison of two concentrations of glycerol in the freezing media. Five adult male mountain lions were used, held at the Rehabilitation Center for Wild Animals of Mato Grosso do Sul (CRAS/MS). Samples were collected by electroejaculation and evaluated for physical appearance, volume, sperm progressive status, sperm motility, sperm concentration and sperm motility index. Each ejaculate was spliced into two aliquots and diluted in Tris-citrate-half egg yolk, at final concentrations of 5 and 7.5% glycerol, cooled at a rate of -0.55°C/min and frozen at a rate of -5.8°C/min. Once thawed, the samples were re-evaluated and tested for thermoresistance and hypoosmotic swelling. The suggested protocol for cryopreservation and thawing of semen is efficient in both glycerol concentrations tested, with no difference (p>0.05) between them.

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Qualidade espermática do sêmen criopreservado de cães: II - Efeito do protocolo de resfriamento

Bueno

Costa

Guimarães

et al. 2001

Arq. Bras. Med. Vet. Zootec.

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Estudou-se o efeito de dois protocolos de resfriamento do sêmen de cães sobre a qualidade espermática in vitro, após sua criopreservação. Utilizaram-se três machos e realizaram-se cinco coletas em cada um deles. Os procedimentos de resfriamento foram realizados em um congelador de células (biocool) e em caixa de isopor. Foram avaliadas a motilidade, o vigor espermático, a integridade da membrana plasmática (teste hiposmótico), a integridade do acrossoma e a longevidade espermática (teste de termo-resistência). A comparação entre os protocolos de resfriamento mostrou que não houve diferença (P>0,05) entre a caixa de isopor e o biocool logo após o resfriamento. Após a descongelação os resultados foram semelhantes entre os protocolos, observando-se diferença (P<0,05) apenas na motilidade espermática e no teste de termo-resistência (TTR), os quais foram superiores na caixa de isopor.

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Qualidade espermática do sêmen criopreservado de cães: I - efeito do meio diluidor

Cited by 5 publications

References 17 publications

Ocelot and oncilla spermatozoa can bind hen egg perivitelline membranes

Ocelot and oncilla spermatozoa can bind hen egg perivitelline membranes

Comparação entre duas concentrações de glicerol para a criopreservação de sêmen de suçuarana (Puma concolor)

Qualidade espermática do sêmen criopreservado de cães: II - Efeito do protocolo de resfriamento

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