Somatic embryogenesis and plant regeneration from zygotic embryo explants of onion

Sivanesan, Iyyakkannu; Kyoung, Kim Eun; Young, Ko Eun; Park, Se W

doi:10.1590/s0102-053620150000400006

Cited by 5 publications

(4 citation statements)

References 24 publications

(6 reference statements)

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“…Embryogenic callus induction in onion has been achieved by using auxins alone in the culture media such as 2,4-D and/or picloram (van der Valk et al 1992;Saker 1997;Eady et al 1998;Zheng et al 1998;Khar et al 2005;Ramakrishnan et al 2013;Sivanesan et al 2015). In our experiment, the callus induction e ciency was low when auxin alone was used either 2,4-D or picloram and it varied from 4 to 22.67% and 24 to 57.33% in shoot tip and seedling radicle explants, respectively.…”

Section: Discussionmentioning

confidence: 63%

“…Bhima super via indirect organogenesis An e cient regeneration protocol, DNA delivery system and a selection system are required for successful genetic transformation of any plant species. Previously reported regeneration protocols for Allium cepa showed the dependence of callus induction on cultivars, explants and media compositions (van der Valk et al 1992;Eady 1995;Saker 1997;Eady et al 1998;Zheng et al 1998;Khar et al 2005;Ramakrishnan et al 2013;Sivanesan et al 2015). In this study, we standardized the regeneration protocol for an Indian cultivar, Bhima super, which is one of the commercially important varieties of onion.…”

Section: Resultsmentioning

confidence: 99%

“…up to 250 mg/L further enhanced the shoot regeneration with highest (73.15%) in 1.5 mg/L kinetin + 0.125 mg/L ABA treatment. This is due to high shoot regeneration e ciency of embryogenic calli induced on 2,4-D-containing media (van der Valk et al 1992;Luthar and Bohanec 1999;Sivanesan et al 2015) and ability of low concentration ABA for increasing the number of shoot-forming embryogenic onion calli in MS medium containing kinetin (van der Valk et al 1992). Khar et al (2005) also reported shoot regeneration in kinetin-containing MS medium from the calli induced from root tip, shoot tip and whole seed explants of onion.…”

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Improved method for regeneration and Agrobacterium-mediated transformation of Indian short-day onion (Allium cepa L.)

Manape

Satheesh

Singh

et al. 2021

Plant Cell Tiss Organ Cult

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A high-auxin medium, usually used for callus induction, was not effective for Indian short-day onion cv.Bhima super. In this study, we found that the onion seedling radicle was a better explant than shoot tip for embryogenic callus induction, and induction e ciency up to 85.33% along with high embryogenic calli weight was obtained in routinely used medium containing 1.0 mg/L 2,4-D, but speci cally supplemented with 0.5 mg/L kinetin. MS medium supplemented with 1.5 mg/L kinetin and 0.125 mg/L ABA showed 73.15% shoot regeneration e ciency from the calli induced from seedling radicle. Geneticin and hygromycin B at 50 mg/L showed optimal selection pressure for 8-week-old onion calli. Agrobacteriummediated transformation of 8-week-old friable embryogenic calli induced from seedling radicle resulted in phenotypically normal transgenic plants with 1% transformation e ciency. In this study, regeneration and transformation protocols were developed for a widely used Indian short-day onion cultivar, which is instrumental for the development of stable transgenics in this crop.

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Section: Discussionmentioning

confidence: 63%

Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Improved method for regeneration and Agrobacterium-mediated transformation of Indian short-day onion (Allium cepa L.)

Manape

Satheesh

Singh

et al. 2021

Plant Cell Tiss Organ Cult

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“…Direct plant regeneration is a valuable approach to reduce the culture duration and to minimize the somaclonal variation extensively (Burner and Grisham 1995;Satish et al 2015). The efficient and reproducible in vitro plant regeneration is a first step for successful biotechnological application in crop improvement to achieve the requirement of food in the shortest period (Parmar et al 2012;Sivanesan et al 2015;Aydin et al 2016). However, the effective utilization of plant tissue culture relies on the interaction between culture media, explants, plant species and environmental growth conditions (Chutipaijit and Sutjaritvorakul 2018).…”

Section: Introductionmentioning

confidence: 99%

Role of activated charcoal and amino acids in developing an efficient regeneration system for foxtail millet (Setaria italica (L.) Beauv.) using leaf base segments

Rathinapriya

Satish

Ramesh

et al. 2018

Physiol Mol Biol Plants

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An efficacious, reproducible direct in vitro regeneration system has been developed from leaf base segments (LBs) of six high yielding genotypes of foxtail millet (Setaria italica (L.) Beauv.). LBs excised from 4-day-old seedling were inoculated on Murashige and Skoog (MS) medium supplemented with different types and concentrations of cytokinins. The shoots induced per explant significantly increased with the supplementation of BAP to auxin containing medium. The results showed that a maximum shoot induction, 58.8% was obtained on MS medium incorporated with 8.9 lM BAP and 2.7 lM NAA in 'CO5' genotype. Further, the highest frequency of multiple shoots was produced on MS(I) medium containing 8.9 lM BAP, 2.7 lM NAA, 700 mg L -1 proline, 0.5 mg L -1 cysteine, 2.0 mg L -1 glycine and 150 mg L -1 arginine. MS(I) medium additionally fortified with 5.0 g L -1 activated charcoal (AC) was found to achieve the best precocious plant regeneration. Elongated shoots were rooted on half-strength MS medium amended with 2.9 lM IAA and achieved maximum root number (8.7) within 10 days. Rooted plantlets were acclimated in soil with 92% survival rate. Molecular marker analysis of in vitro regenerated and field grown plants revealed no somaclonal variations. Briefly, amino acids and activated charcoal could significantly enhance the foxtail millet direct multiple shoot proliferation and plant regeneration. Here we report, a short-term, genotype independent, direct plant regeneration protocol for future genetic transformation studies in foxtail millet genotypes.

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In vitro regeneration of Piper longum L. and comparative RP-HPLC analysis of piperine production of in vitro and in vivo grown plants

Chatterjee

Chandra

2022

Plant Cell Tiss Organ Cult

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Piper longum L. is a well known spice plant belonging to the family Piperaceae with high pharmacognosy potential, but it is becoming threatened due to overexploitation. Thus, this investigation aims to standardize a cost effective protocol for in vitro propagation of this economically important plant.Internodal segments were used as explant for callogenesis in Murashige and Skoog medium with 3% sucrose and 0.8% agar, with NAA or 2,4 D. Optimum callus induction was observed in MS medium with 5.0 mg/L NAA. Calli were subcultured on shoot regeneration media containing different concentrations of cytokinin (KIN/BAP) along with 0.1mg/L NAA. Best shoot regeneration was obtained on MS media supplemented with 2.0 mg/L KIN and 0.1mg/L NAA. Induced shoots were rooted in either NAA or IBA and highest rooting was induced in MS medium enriched with 0.5 mg/L NAA. Rooted plantlets were acclimatized and 88% of hardened plants survived. Field emission scanning electron microscopic (FE-SEM) showed that regeneration from callus had occurred by somatic embryogenesis. A comparative study on identi cation and quanti cation of piperine ( the chief alkaloid of the cultivar) were done from root and fruit of both in vitro and in vivo grown plants through Reverse Phase-High Performance Liquid Chromatography (RP-HPLC) method. I n vitro grown fruit was found to have the maximum amount of piperine. Key MessageFor the rst time, a comparative study in identi cation and quanti cation of piperine was done from both in vitro and in vivo grown Piper longum through RP-HPLC method.

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Somatic embryogenesis and plant regeneration from zygotic embryo explants of onion

Cited by 5 publications

References 24 publications

Improved method for regeneration and Agrobacterium-mediated transformation of Indian short-day onion (Allium cepa L.)

Improved method for regeneration and Agrobacterium-mediated transformation of Indian short-day onion (Allium cepa L.)

Role of activated charcoal and amino acids in developing an efficient regeneration system for foxtail millet (Setaria italica (L.) Beauv.) using leaf base segments

In vitro regeneration of Piper longum L. and comparative RP-HPLC analysis of piperine production of in vitro and in vivo grown plants

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