In vitro regeneration of Piper longum L. and comparative RP-HPLC analysis of piperine production of in vitro and in vivo grown plants

Abstract

Piper longum L. is a well known spice plant belonging to the family Piperaceae with high pharmacognosy potential, but it is becoming threatened due to overexploitation. Thus, this investigation aims to standardize a cost effective protocol for in vitro propagation of this economically important plant.Internodal segments were used as explant for callogenesis in Murashige and Skoog medium with 3% sucrose and 0.8% agar, with NAA or 2,4 D. Optimum callus induction was observed in MS medium with 5.0 mg/L NAA. Calli were subcultured on shoot regeneration media containing different concentrations of cytokinin (KIN/BAP) along with 0.1mg/L NAA. Best shoot regeneration was obtained on MS media supplemented with 2.0 mg/L KIN and 0.1mg/L NAA. Induced shoots were rooted in either NAA or IBA and highest rooting was induced in MS medium enriched with 0.5 mg/L NAA. Rooted plantlets were acclimatized and 88% of hardened plants survived. Field emission scanning electron microscopic (FE-SEM) showed that regeneration from callus had occurred by somatic embryogenesis. A comparative study on identi cation and quanti cation of piperine ( the chief alkaloid of the cultivar) were done from root and fruit of both in vitro and in vivo grown plants through Reverse Phase-High Performance Liquid Chromatography (RP-HPLC) method. I n vitro grown fruit was found to have the maximum amount of piperine. Key MessageFor the rst time, a comparative study in identi cation and quanti cation of piperine was done from both in vitro and in vivo grown Piper longum through RP-HPLC method.

Secondary Metabolites Profiling, Antimicrobial and Cytotoxic Properties of Commiphora gileadensis L. Leaves, Seeds, Callus, and Cell Suspension Extracts

“…These results agree with Xiong et al [28], where the high concentration of NAA and BAP in combination enhanced callus growth in Cordia subcordata. In addition, high NAA concentrations were used successfully in promoting callus growth in Piper longum [29]. In another study, Daffalla et al [30] found that a combination of 10.74 µM NAA with 6.66 µM BAP increased the callus growth of Grewia tenax, and this is in general agreement with the results of this study.…”

The Optimization of Medium Conditions and Auxins in the Induction of Adventitious Roots of Pokeweed (Phytolacca americana L.) and Their Phytochemical Constituents

“…These propagated materials, with or without elicitors, can be utilized to extract bioactive compounds. Fruits of in vitro grown plants of Piper longum and cell suspensions of Cnidoscolus chayamansa showed greater contents of piperine and lupeol acetate than in vivo plants, respectively [ 17 , 18 ]. There is a report that investigated the in vitro production of betacyanin from pokeweed cell suspension [ 19 ].…”