Characterization of new variety of Chrysanthemum by using ISSR markers

Palai, SK; Rout, Gyana Ranjan

doi:10.1590/s0102-05362011000400029

Cited by 19 publications

(10 citation statements)

References 17 publications

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“…The color change of plant tissue is one of the commonly observed phenotypes of natural mutation. The mechanisms of color change in some plants have been studied extensively (Tegopati, 2001;Li et al, 2005;Yamaguchi et al, 2009;Palai and Rout, 2011), but not in the sport varieties of the European pear. In this paper, we studied the mechanism of the formation of green-skinned MRB-G fruit and demonstrated that hypermethylation of the PcMYB10 promoter was probably the causative factor.…”

Section: Discussionmentioning

confidence: 99%

The Methylation of the PcMYB10 Promoter Is Associated with Green-Skinned Sport in Max Red Bartlett Pear

et al. 2013

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Varieties of the European pear (Pyrus communis) can produce trees with both red-and green-skinned fruits, such as the Max Red Bartlett (MRB) variety, although little is known about the mechanism behind this differential pigmentation. In this study, we investigated the pigmentation of MRB and its green-skinned sport (MRB-G). The results suggest that a reduction in anthocyanin concentration causes the MRB-G sport. Transcript levels of PcUFGT (for UDP-glucose:flavonoid 3-O-glucosyltransferase), the key structural gene in anthocyanin biosynthesis, paralleled the change of anthocyanin concentration in both MRB and MRB-G fruit. We cloned the PcMYB10 gene, a transcription factor associated with the promoter of PcUFGT. An investigation of the 2-kb region upstream of the ATG translation start site of PcMYB10 showed the regions 2604 to 2911 bp and 21,218 to 21,649 bp to be highly methylated. A comparison of the PcMYB10 promoter methylation level between the MRB and MRB-G forms indicated a correlation between hypermethylation and the green-skin phenotype. An Agrobacterium tumefaciens infiltration assay was conducted on young MRB fruits by using a plasmid constructed to silence endogenous PcMYB10 via DNA methylation. The infiltrated fruits showed blocked anthocyanin biosynthesis, higher methylation of the PcMYB10 promoter, and lower expression of PcMYB10 and PcUFGT. We suggest that the methylation level of PcMYB10 is associated with the formation of the greenskinned sport in the MRB pear. The potential mechanism behind the regulation of anthocyanin biosynthesis is discussed.

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Section: Discussionmentioning

confidence: 99%

The Methylation of the PcMYB10 Promoter Is Associated with Green-Skinned Sport in Max Red Bartlett Pear

et al. 2013

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“…ISSR gained popularity because they are highly polymorphic, flank highly conserved sequences, and are very useful for assessing natural variation in plants (Sarwat, 2012). Multiple papers confirm the effectiveness of ISSR in different species, such as chrysanthemum (Palai and Rout, 2011), grape (Hassan et al, 2011), and rose (Jabbarzadeh et al, 2010). Today, ISSR are considered one of the best options for DNA fingerprinting, assessment of genetic diversity between closely related individuals, genetic stability, gene tagging, hybrid and cultivar identification, somaclonal variation and production quality (Jabbarzadeh et al, 2010;Chatti et al, 2010;Sarwat, 2012).…”

Section: Introductionmentioning

confidence: 95%

Initial assessment of natural diversity in Mexican fig landraces

García-Ruiz¹,

Mendoza-Castillo²,

Valadez-Moctezuma³

et al. 2013

Genet. Mol. Res.

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ABSTRACT. The common fig (Ficus carica L.) was introduced intoMexico by Spanish Franciscan missionaries in the 16th century. It is widely assumed that Mexican figs are the Spanish cultivar Black Mission. We collected and propagated 12 fig plants from six landraces from different states in Central Mexico that represent different climate. All of them were grown in a greenhouse at Universidad Autónoma Chapingo, in the State of Mexico. During the experimental period, the greenhouse had an average temperature and relative humidity of 29.2° ± 5.4°C (SEM) and 78.1 ± 6.7% (SEM), respectively. Morphological characterization was done following a selected set of quantitative and qualitative descriptors established by the IPGRI. DNA analysis was based on a combination of ISSR and RFLP markers. We observed great diversity mainly in fruit weight (28.1-96.2 g), fruit shape (ovoid, pyriform), and neck length (0.97-3.80 cm), which could not be explained by environmental conditions such as temperature and relative humidity. The Nei and Li/Dice similarity coefficient between landraces was determined by cluster analysis using the UPGMA method. Based on the morphological characterization and DNA fingerprinting data presented in this study, our results showed that after hundreds of years, black figs have adapted to local environmental condition in Central Mexico, yielding at least six clearly distinct landraces that represent valuable and previously undescribed genetic diversity. We also suggested names for those landraces according to their location and established a basis for further agronomic and molecular characterization of fig landraces.

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“…Molecular analysis was performed using six different ISSR primers (Genomed S.A., Poland). Six PCR reactions were performed for 14 genotypes (in two repetitions) as follows: − S1 -sequence 5'→ 3' GAGGGTGGAGGATCT [Palai and Rout 2011]; − S2 -sequence 5'→ 3' CGAGAGAGAGAGAGAGA [Palai and Rout 2011]; − S3 -sequence 5'→ 3' GTGAGAGAGAGAGAGAGA [Mukherjee et al 2013]; − S4 -sequence 5'→ 3' GACAGACAGACAGACA [Mukherjee et al 2013]; − S5 -sequence 5'→ 3' GAGAGAGAGAGAGAGAGAT [Mukherjee et al 2013]; − S6 -sequence 5'→ 3' CAGAGAGAGAGAGAGAG [Palai and Rout 2011].…”

Section: Issr Analysismentioning

confidence: 99%