Desenvolvimento de Eurytrema coelomaticum (Giard & Billet) (Digenea, Dicrocoeliidae) em Bradybaena similaris (Férussac) (Gastropoda, Xanthonychidae)

Abstract

ABSTRACT. Development of ElIrylrellla coel omaliclIlII (Giard & Billet) (Digenea, Dicrococliidac) in Brat/ybaella silllilaris (Férussac) (Gastropoda, Xanthonychidae). To follow the larval development of Ewytrema coelomaliclIm (Giard & Billel, 1892) in 8radybaena similaris (Férussac, 1821) snails were separaled in three classes llsing lhe shell diameter: Class A (14.5-10 .2 mm), Class B (10.1-6.9 mm) and Class C (6.8-2.6 mm). Only snails belonging to classes A and B acquired the inteclion.Specimens of E. coelomalicum removed from lhe pancrealic ducts were exposed to lhree physiological solutions: Earle, Locke and saline 0.85%, to obtain eggs for the experimental infections. The Locke solution induced the best egg release. The route ofmigration lhe intramolluscan development oI' E. coelomalicum was stlldied with the aid 01" histology. The minimal period of intramolluscan development, ending aI lhe expelling of daughler sporocysts, was 107 days for the snails intecled in March, and 79 days for lhe snails infecled in November. The Sludenl "t" test and the Chi-square lest showed a significant difterence (u = 5%) between the two periods, although the mean lemperalure regislered during the experiments did not signiticanlly diftered (u = 5%). The eliminalion of daughter sporocysls occurred lhrough lhe snail's pneumostome, and always aI night. Most sporocysts were eliminated aI intervals thal varied between one to three days, without reglllarity. The time ofelimination ofthe dallghter sporocysts was ditlerenl for the two intection period studied: 12 weeks for the snails infecled in March, and llu'ee weeks for lhos e infecled in November. Posilive correlalion between the number of sporocysts expelled by the snail hOSI and higher lemperatures registered in lhe laboralOlY was observed. This correlation was more evident in November infeclion. KEY WORDS.

Molecular characterization of Eurytrema coelomaticum in cattle from Paraná, Brazil

“…in Brazil have been based on morphological characterization (TRAVASSOS et al, 1969;YAMAMURA et al, 1995;PINHEIRO et al, 2012), biological cycle (BRANDOLINI; AMATO, 2001), epidemiological trends (AZEVEDO et al, 2004;BASSANI et al, 2006), and pathological manifestations (YAMAMURA et al, 1995;HEADLEY, 2000;HEADLEY et al, 2009). Additionally, there are also descriptions of Eurytrema spp.…”

Digital image analysis to estimate the minimum number of Eurytrema coelomaticum eggs in the uterus of adult specimens

“…In the literature, there is no description of estimates of total number of eggs present in the uterus of E. coelomaticum . There are studies that evaluated the oviposture rate of the parasite in vitro , and these studies observed a deposition ranging from 194 to 746 eggs per day in different physiological solutions (Yamamura, 1989; Brandolini and Amato, 2001). According to Bassani et al (2007) the proportion of eggs eliminated and observed via EPG is not directly related to number of parasites in the host, even considering those who lay their eggs directly in lumen of intestine.…”

“…Yamamura (1989) analyzing the oviposture of E. coelomaticum ( in vitro ) observed an average daily of 641 eggs. Brandolini and Amato (2001) testing different physiological media observed that Locke solution presented the best oviposture rate with an average of 746 ± 139 eggs, followed by Earle solution with 304.6 ± 154.7 and finally the saline solution to 0.85 % with 194.5 ± 132 eggs. About this information, there are no records in the literature of estimate number of eggs present in the uterus of E. coelomaticum .…”

An improvement of calcium determination technique in the shell of molluscs

“…Many physiological alterations may affect the calcium reserves in the shell and in the hemolymph of mollusks (Souza et al, 2000;Lira et al, 2000;Pinheiro, 1996). Calcium determination in the shell of the snails is an important parameter that is widely used as an indirect evaluation of metabolic alterations, influence of the environment and conditions of stress on the physiology of molluscs (Paschoal and Amato, 1996;Brandolini and Amato, 2001;Moreira et al, 2003). The calcium present in the shell is predominantly found as calcium carbonate incorporated in the shell structure as calcite and aragonite crystals associated to the organic matrix, composed basically by conchiolin.…”