Molecular characterization of Eurytrema coelomaticum in cattle from Paraná, Brazil

Abstract

This study investigated the occurrence of Eurytrema spp. in cattle by analysis of the partial 18S rRNA gene sequence. Trematodes from 44 bovine pancreas were collected and classified based on typical morphological features. PCR assay and sequence analyses of amplified products confirmed that the trematodes classified as Eurytrema coelomaticum were phylogenetically distinct from those identified as E. pancreaticum. The results of this study represent the first molecular characterization of E. coelomaticum within the Americas, and provide an efficient method to differentiate digenean trematodes of domestic animals.Keywords: Bovine trematode, Eurytrema spp., PCR, phylogeny. ResumoEste estudo avaliou a ocorrência de Eurytrema spp. em bovinos por meio da análise da sequência parcial do gene 18S rRNA. Trematódeos procedentes de 44 pâncreas bovinos foram coletados e classificados com base em aspectos morfológicos típicos. A técnica de PCR e a análise da sequência de nucleotídeos dos produtos amplificados confirmaram que os trematódeos classificados como Eurytrema coelomaticum eram filogeneticamente distintos daqueles identificados como E. pancreaticum. Os resultados deste estudo representam a primeira caracterização molecular de E. coelomaticum nas Américas e disponibiliza um método eficiente para diferenciar trematódeos digenéticos em animais domésticos.Palavras-chave: Trematoda bovino, Eurytrema spp., PCR, filogenia.

Sequencing and characterization of the complete mitochondrial genome from the pancreatic fluke Eurytrema pancreaticum (Trematoda: Dicrocoeliidae)

“…Phylogenetic analysis of the 12 mt proteins concatenated amino acid sequence data ( Jiraungkoorskul et al, 2005;Bassani et al, 2006;Pinheiro et al, 2011Pinheiro et al, , 2012Franco-Acuña et al, 2011); however, few molecular data are presently available (Zheng et al, 2007;Cai et al, 2012;Figueira et al, 2014;Mohanta et al, 2015). Therefore, our characterization of the E. pancreaticum mt genome will provide a foundation for the improved identification of E. pancreaticum and other trematodes using molecular methods.…”

Eurytrematosis: a Review of the Pancreatic Fluke

“…There are many of fluke members of the genus Eurytrema (Class: Trematoda, Family: Dicrocoeliidae) have been identified as the most serious internal parasite problem of ruminants in the world. Base on typical morphological characteristics and molecular properties, Eurytrema coelomaticum has been identified in cattle from southern Bazil (Figueira et al, 2014). Similarly, Mohanta et al (2015) have been confirmed the existence of Eurytrema cladorchis fluke recovered from the bile duct of cattle (Bos indicus) in Bandarban, a hill district in Bangladesh.…”

Morphology and molecular identification of Eurytrema spp. worm in Aceh cattle, Indonesia

“…Mohanta et al (2015) was to identify Eurytrema flukes both by morphology and molecular properties on the basis of 18-subunit ribosomal RNA (18S rRNA) gene as well as internal transcribed spacer 2 (ITS2) to clarify their phylogenetic status. Figueira et al (2014) also identified unique morphological characteristics and molecular features in E. coelomaticum worms found in cattles in South Brazil. Mohanta et al (2015) confirmed the presence of E. cladorchis in the biliary tract of cattle (Bos indicus) in Bandarban a mountainous district in Bangladesh.…”