Características hematológicas do tambaqui Colossoma macropomum Cuvier (Osteichthyes, Characidae) em sistema de monocultivo intensivo: II. Leucócitos

Tavares‐Dias, Marcos; Sandrim, Elziane F. Silva; Campos-Filho, Eugênio de

doi:10.1590/s0101-81751999000100012

Cited by 20 publications

(12 citation statements)

References 27 publications

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“…,‐1999; Tavares‐Dias & Sandrin ; Tavares‐Dias et al . ). Due to the lack of effect of AP supplementation on growth parameters, we may assume that tambaqui fed the diets with the lowest AP levels was under marginal deficiency which could be detected by the haematology data.…”

Section: Discussionmentioning

confidence: 97%

Dietary available phosphorus requirement for tambaqui,Colossoma macropomum, juveniles based on growth, haematology and bone mineralization

et al. 2016

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An economical and efficient approach to reduce the impact of P discharge by aquaculture industry is to adjust the P level in fish feeds to the precise nutrient requirement at different growth stages in a digestible nutrient basis. However, P requirement seems to be species specific and affected by several physiological, dietary and environmental factors. Based on the importance of tambaqui (Colossoma macropomum) to Latin American aquaculture, we designed a 63-day trial to evaluate the effect of available P (AP) levels on growth, nutrient digestibility, haematology and blood biochemical parameters, carcass proximate composition and bone mineralization. Quadruplicate groups of tambaqui juveniles (144 AE 2.0 g) were fed five isonitrogenous (278 g kg À1 digestible protein) and isocaloric (13.5 Mj DE kg À1 ) diets containing graded AP levels (3.0, 5.6, 7.5, 9.1 and 11.0 g kg À1 ) following a completely randomized design. Dicalcium phosphate (DCP) was used as the main P source. No mortalities and signs of P deficiency were observed among the dietary treatments. A remarkably high P digestibility was observed in all plantbased diets with a tendency of decreasing P digestibility with the increase in total P levels. Tambaqui seems to be able to grow well without inorganic P supplementation during the trials; however, this species required 7.0 g AP kg À1 diet for proper bone mineralization. P supplementation had a limited effect on haematology and blood biochemistry of tambaqui. Ecological implications of natural feeding habit and evolutionary position of this species are further discussed, and new hypothesis are drawn based on our results.

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Section: Discussionmentioning

confidence: 97%

Dietary available phosphorus requirement for tambaqui,Colossoma macropomum, juveniles based on growth, haematology and bone mineralization

et al. 2016

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“…The total WBC counts (WBC) were later enumerated in a hemacytometer (Improved Neubauer, Model BS-713, Weber Scientific Limited, Middlesex, UK) using Shaw's diluting fluid (Miale 1982). Blood smears were stained with Grumwald-Giemsa for differential examination (Tavares Dias et al 1999).…”

Section: Methodsmentioning

confidence: 99%

Select Hematological Values of the African Catfish (Clarias gariepinus) Raised in a Water Recirculating Aquaculture System

Akinrotimi

Bekibele

Orokotan³

2011

International Journal of Recirculating Aquaculture

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Clinical evaluation of blood parameters is routinely used to assess the health of wild and domestic animals. The commercial catfish industry in Nigeria has undergone rapid expansion in recent years. An understanding of normal hematology values for healthy fish and the identification of predictors of the onset of health problems may enable fish health specialists to intervene before major losses occur. This paper reports values for selected hematological parameters of normal healthy African catfish (Clarias gariepinus) (n=120) raised in a recirculating aquaculture water recirculation system, including hemoglobin (Hb), red blood cells (RBC), packed cell volume (PCV), white blood cells (WBC), mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC), thrombocyte count, and leukocyte differential (lymphocytes, neutrophils and monocytes). Significant differences (p< 0.05) were observed between males and females in the values of Hb, PCV, and thrombocytes. This work provides hematological baselines for selected values for Clarias gariepinus in recirculation systems, and is intended to enhance production performance through early detection and identification of infectious diseases.

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“…Blood smears were prepared and panchromatically stained with a combination of May Grünwald-Giemsa-Wright (Ranzani-Paiva et al, 2013) stains for the leucocyte differential counting up to 200 cells in each blood smear. The identification and classification of the leucocyte populations were made according to the recommendations of Tavares- Dias et al (1999). The blood smears were also used to count the total number of leucocytes and thrombocytes (Ranzani-Paiva et al, 2013).…”

Section: Fish and Acclimatizationmentioning

confidence: 99%

Antiparasitic activity, histopathology and physiology of Colossoma macropomum (tambaqui) exposed to the essential oil of Lippia sidoides (Verbenaceae)

Soares

Neves²,

Ferreira³

et al. 2017

Veterinary Parasitology

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In vivo and in vitro antiparasitic activity of the essential oil of Lippia sidoides and blood and histological alterations were assessed in Colossoma macropomum (tambaqui). Essential oil concentrations of 10, 20, 40, 80, 160 and 320mg/L were assayed in vitro against monogenoideans Anacanthorus spathulatus, Notozothecium janauachensis and Mymarothecium boegeri from fish gills. Lippia sidoides essential oil concentrations of 320 and 160mg/L were 100% effective against monogenoideans in 10min and 1h of exposure, respectively. However, the effectiveness of 100% concentrations of 80mg/L and 40mg/L occurred in 3 and 6h, respectively. In the in vivo tests, juvenile fish were submitted to 60min of baths with 10mg/L and 15min of baths with 20mg/L of the essential oil of L. sidoides. These therapeutic baths were not efficient against Ichthyophthirius multifiliis, and monogenoideans present in the gills of C. macropomum. In addition, 10 and 20mg/L of the essential oil of L. sidoides caused an anesthetic effect on the fish and did not influence total glucose and protein plasma levels; however, it decreased the number of total erythrocytes in fish exposed to the higher concentration of this essential oil. Severe alterations and irreversible damage were observed in the fish gills just after L. sidoides essential oil baths and after 24h of recovery. The most recurrent lesions found were hyperplasia and fusion of the lamellar epithelium, vasodilation, detachment of the gill epithelium and lamellar aneurism, epithelial breakdown with hemorrhage, congestion, edema and necrosis, proliferation of the mucous cells and chloride cells and lamellar hypertrophy. Therefore, since the essential oil of L. sidoides has in vitro antiparasitic activity and low concentrations of it have shown toxic effects, the bioactive potential of its main chemical components should be investigated, as well as more efficient forms of its administration in therapeutic baths in order to eliminate fish parasites.

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Características hematológicas do tambaqui Colossoma macropomum Cuvier (Osteichthyes, Characidae) em sistema de monocultivo intensivo: II. Leucócitos

Cited by 20 publications

References 27 publications

Dietary available phosphorus requirement for tambaqui,Colossoma macropomum, juveniles based on growth, haematology and bone mineralization

Dietary available phosphorus requirement for tambaqui,Colossoma macropomum, juveniles based on growth, haematology and bone mineralization

Select Hematological Values of the African Catfish (Clarias gariepinus) Raised in a Water Recirculating Aquaculture System

Antiparasitic activity, histopathology and physiology of Colossoma macropomum (tambaqui) exposed to the essential oil of Lippia sidoides (Verbenaceae)

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