Effect of storage period on isoflavone content and physiological quality of conventional and transgenic soybean seeds

Abstract

-The objective in this research was to evaluate the isoflavone content and the physiological quality of seed from conventional and transgenic soybean cultivars before and after 180 days of storage. Twenty one soybean cultivars: CD 202, CD 206, CD 208, CD 213RR, CD 214RR, CD 215, CD 216, CD 217, CD 218, CD 221, BRS 184, BRS 185, BRS 214, BRS 244RR, BRS 245RR, BRS 246RR, BRS 255, BRS 257, BRS 258, BRS 261 and BRS 262, grown in the 2005/2006 crop season, were assayed. The seeds were packed in Kraft paper bags and stored at room temperature under laboratory conditions. Seeds were evaluated with respect to their germination and vigor (first germination count, accelerated aging and tetrazolium test) and their total isoflavone contents and respective aglycon forms (daidzein, genistein and glycitein),glycosides (daidzine, genistine and glycitine) and malonyl conjugates. A completely randomized block design with six replications with the treatments set out within a subplot scheme (21 cultivars x 2 storage periods) was used. The F-test was used to compare means between storage periods and the Scott-Knott test to compare cultivars for each storage period, both with a 95% probability. It was concluded that isoflavone contents differ between cultivars and show a distinct behavior throughout storage. isoflavonas totais e suas respetivas formas agliconas (daidzeína, genisteína e gliciteína), glicosídeos (daidzina, genistina e glicitina) e seus conjugados malonil. O delineamento experimental utilizado foi o inteiramente casualizado com 6 repetições e tratamentos dispostos no esquema de parcelas subdivididas (21 cultivares x 2 tempos de armazenamento). Para a comparação de médias entre tempos de armazenamento, utilizou-se o teste F e para comparações de cultivares para cada tempo de armazenamento utilizou-se o teste de agrupamento de Scott-Knott, ambos com 95% de probabilidade. Pelos resultados foi possível concluir que os teores de isoflavonas diferenciam-se entre as cultivares e possuem comportamento distinto ao longo do armazenamento.Termos para indexação: agliconas, Glycine max, daidzina, genistina, vigor.

Correlations and path analysis between fruit characteristics and seeds of Pachira aquatica Aubl.

“…Except for weight of a seed (SW), the table shows significant statistical differences (P<0.05 and highly significant P<0.01) among the 10 trees, for the variables: fresh fruit weight (FFW), fruit length (FL), fruit peel weight (FPW), number of seeds per fruit (NSF), weight of seeds per fruit (WSF), percentage of seeds per fruit (PSF), volume of a seed (SV) and density of a seed (SD), which shows genetic differences between trees, a consistent result with the reported by Silva et al (2012a) en P. aquatica and in agreement with the concepts of Pereira et al (2011) in Hymenaea stigonocarpa, with different number of tree, who point out that tropical trees show great variability in the mentioned characteristics. percentage of seeds per fruit; SW: weight of a seed; SV: volume of a seed; SD: density of a seed; CV: coefficient of variation; L.S.…”

“…Large and heavy seeds contain higher amounts of carbohydrates in the endosperm or cotyledons, and are available as a source of energy to stimulate seedling germination, emergence, survival and growth (Pereira et al, 2011). These correlations have been reported by HuertaPaniagua and Rodríguez-Trejo (2011). However, other studies do not report such associations (Binotto et al, 2010) and may vary between species and between regions (Pereira et al, 2011).…”