Eficiência dos testes de vigor na avaliação da qualidade fisiológica de sementes de mulungu (Erythrina velutina WILLD.)

Bento, Sílvia Regina Silva de Oliveira; Santos, Ademir dos; Melo, Dalila Regina Mota de; Torres, Salvador Barros

doi:10.1590/s0101-31222010000400012

Cited by 6 publications

(2 citation statements)

References 13 publications

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“…Similar values of length, width, and thickness were observed in studies that evaluated two seed lots from the semiarid regions of Rio Grande do Norte, with 13.0 to 13.4 mm for the length, 8.0 to 8.6 mm for the width, and 7.8 to 8.1 mm for the thickness, and from a lot in Paraíba, with an average of 12.54 mm for the length, 8.15 mm for the width, and 7.66 mm for the thickness (Silva et al, 2008;Bento et al, 2010).…”

Section: Physical Qualitymentioning

confidence: 99%

Seed Viability and RNA Extraction Methods of Erythrina velutina Under Drought Stress

Souza¹,

Freitas²,

Lima³

et al. 2020

JAS

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Abiotic stress caused by rigorous environmental conditions, such as drought, has negative effects on seeds. The species from arid and semiarid areas have mechanisms to maintain seed viability. In this study, the analyses of physical and physiological quality of the seeds of Erythrina velutina and their response to drought stress and RNA extraction methods were carried out. The seeds were collected from mother trees in two different provenances of natural occurrences of the species. The physical quality, viability, and vigor of the seeds were evaluated by morphometric parameters, germination, and electrical conductivity tests. The seeds were submitted to drought stress conditions for 0, 24, 48, and 72 hours on -0.8 MPa polyethylene glycol solution. To evaluate the viable RNA extraction method, the seed embryos were used for the extraction of this molecule and with an assessment of quantity and quality. The seeds from Pernambuco that were harvested in 2008 were bigger and presented lower vigor when in comparison to seeds that were harvested from Sergipe in 2012. The seeds under drought stress conditions presented null germination for all the treatments. After the drought stress, they were germinated on a paper substrate moistened with water, with better responses from the Lot Sergipe, 2012. The imbibition of seeds in the solution for 24 hours provides an increase in germination for less vigorous seeds, being indicated as a pre-germinative treatment. The RNA quality obtained by using a commercial extraction kit has better results, providing materials without contaminants when compared to the TRIzol method.

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Section: Physical Qualitymentioning

confidence: 99%

Seed Viability and RNA Extraction Methods of Erythrina velutina Under Drought Stress

Souza¹,

Freitas²,

Lima³

et al. 2020

JAS

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show abstract

“…results (Barbieri, Menezes, Conceição, & Tunes, 2012), as the application of these evaluations for different species is routine in laboratories of research or seed analysis and should allow the results obtained to be compared and reproduced (Bento, Santos, Melo, & Torres, 2010).…”

Section: Introductionmentioning

confidence: 99%

Controlled deterioration test to evaluate the physiological potential of sweet corn seeds

Marinho

Bazzo

Zucareli

et al. 2021

Semina: Ciênc. Agrár.

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The evaluation of the physiological potential of sweet corn seeds is essential in a quality control program and the choice of vigor tests using an adequate analysis methodology is essential to obtain reliable results. In this sense, this study aimed to standardize the methodology of the controlled deterioration test through combinations between time and temperature to evaluate the vigor of sweet corn seeds. Six lots of sweet corn seeds were characterized by the degree of moisture and the initial physiological potential through the following tests: germination, first germination count, cold test, electrical conductivity, accelerated aging, length and dry mass of normal seedlings, seedling emergence in sand, and emergence rate index. Subsequently, these lots had their initial water content adjusted to 20% using the wet substrate method. The seeds from the six lots were subjected to the controlled deterioration test under two temperatures for four periods, obtaining a completely randomized design in an 8 × 6 factorial scheme with eight combinations of time/temperature and six lots. Germination and the first germination count were evaluated after the deterioration test. The data were subjected to analysis of variance with a comparison of means by the Tukey test at a 5% significance. The results of the first count and germination tests after deterioration were subjected to a simple linear correlation study together with the initial characterization data. The combinations 8 h – 40 °C and 8 h – 45 °C in the controlled deterioration test were efficient in distinguishing the vigor of seed lots. The shorter exposure period and the first germination count after deterioration are more suitable for evaluating the vigor of sweet corn seeds.

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Erythrina velutina Willd. Fabaceae

Ferreira

Magalhães²,

Souza³

et al. 2022

Ethnobotany of Mountain Regions

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Eficiência dos testes de vigor na avaliação da qualidade fisiológica de sementes de mulungu (Erythrina velutina WILLD.)

Cited by 6 publications

References 13 publications

Seed Viability and RNA Extraction Methods of Erythrina velutina Under Drought Stress

Seed Viability and RNA Extraction Methods of Erythrina velutina Under Drought Stress

Controlled deterioration test to evaluate the physiological potential of sweet corn seeds

Erythrina velutina Willd. Fabaceae

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