Avaliação da viabilidade e do vigor de sementes de pepino pelo teste de tetrazólio

Lima, Liana Baptista de; Pinto, Taís Leite Ferreira; Novembre, Ana Dionisia da Luz Coelho

doi:10.1590/s0101-31222010000100007

Cited by 14 publications

(17 citation statements)

References 6 publications

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“…Las semillas registraron variaciones en la intensidad de la tinción, debido a que la sal de tetrazolio permite determinar la presencia, localización y naturaleza de las alteraciones de los tejidos de las semillas (Lima et al, 2010;ISTA, 2014), produciéndose en los tejidos vivos trifenil formazan, que identifica la actividad respiratoria de las mitocondrias, y como resultado muestra que hay viabilidad celular. Por lo tanto, el color rojo en los embriones es un indicador positivo de la viabilidad de las semillas (Craviotto et al, 2008), por lo que, se utiliza en programas de control de calidad de semillas; y aquellas regiones débilmente coloreadas en algunas partes del embrión, indican que las células presentan una disminuida actividad respiratoria y, por consiguiente, menor actividad de enzimas deshidrogenasas (Rao et al, 2007;Rodríguez et al, 2008).…”

Section: Determinación De Patrones Topológicosunclassified

“…La comparación de medias (Cuadro 2) y las intensidades de tinción de los embriones (Figura 5) resaltaron que la concentración de 1% de tetrazolio y tiempo de tinción de dos horas, fueron eficientes para evaluar la calidad de semillas, dado que las primeras horas de absorción de agua por la semilla son importantes, ya que se relacionan con la actividad enzimática y por lo tanto, con la coloración final (Lima et al, 2010). Las concentraciones de 0,5% por dos y tres horas fueron las que presentaron los mayores porcentajes de viabilidad; sin embargo, están sobre estimadas (al comparar con la capacidad germinativa), mientras que al 1% por tres horas subestima el porcentaje de viabilidad.…”

Section: Evaluación De Diferentes Concentraciones De Tetrazolio Y Tieunclassified

“…Las concentraciones de 0,5% por dos y tres horas fueron las que presentaron los mayores porcentajes de viabilidad; sin embargo, están sobre estimadas (al comparar con la capacidad germinativa), mientras que al 1% por tres horas subestima el porcentaje de viabilidad. La concentración de tetrazolio al 1% durante dos horas, se ratificó mediante las pruebas de germinación, en las cuales los porcentajes estimados no presentaron diferencias significativas con los valores obtenidos a través de los tratamientos de la prueba bioquímica (Cuadro 2), y fueron consistentes con los reportados en ahuyama (Barros et al, 2005) y en pepino (Lima et al, 2010). …”

Section: Evaluación De Diferentes Concentraciones De Tetrazolio Y Tieunclassified

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Características morfométricas, anatómicas y viabilidad de semillas de Cedrela odorata L. y Cariniana pyriformis Miers*.

Camacho

Tatis²,

Ayala³

2017

Mesoam. Agron.

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© 2017 Agronomía Mesoamericana es desarrollada en la Universidad de Costa Rica y se encuentra licenciada con Creative Commons Reconocimiento-NoComercial-SinObraDerivada 3.0 Costa Rica. Para más información escríbanos a pccmca@ucr.ac.cr ResumenEl objetivo del estudio fue describir las características morfométricas, anatómicas y la viabilidad de las semillas de Cedrela odorata y Cariniana pyriformis. El trabajo se llevó a cabo en la Universidad de Córdoba de Montería, Colombia, en el período comprendido entre abril de 2012 a julio de 2014; con una metodología descriptiva de laboratorio, la prueba de tetrazolio y la prueba de germinación en cámara germinativa. Se utilizaron semillas de lotes comerciales de tres localidades del departamento de Córdoba. Se seleccionaron al azar cinco árboles de cada especie y se tomaron al azar cinco muestras de cien semillas de cada uno. La descripción de la anatomía se hizo con base en diez semillas de cada especie. Para la prueba de tetrazolio, se estableció un experimento bajo un diseño completamente al azar, con seis tratamientos y cuatro repeticiones de veinticinco semillas. Los seis tratamientos se originaron al combinar las concentraciones de tetrazolio de 0,5, 1,0 y 1,5%, con dos y tres horas de inmersión en la solución. Las características externas de C. odorata presentaron menor variación que las de C. pyriformis, especialmente aquellas relacionadas con el peso. Cuatro patrones topológicos fueron identificados en las semillas de cada especie. Se pudo determinar la viabilidad para C. pyriformis y C. odorata, con inmersiones por dos horas en concentraciones de 0,5 y 1,0% de tetrazolio, respectivamente.Palabras clave: calidad de semilla, germinación de semillas, prueba de tetrazolio. AbstractThe aim of the study was to identify morphometric, anatomical, and the viability characteristics of the seeds of Cedrela odorata and Cariniana pyriformis. The study was carried out at the Universidad de Córdoba of Montería, Colombia, from April 2012 to July 2014; the study was carried out with a laboratory descriptive methodology, tetrazolium and germination tests in germination chamber were performed. Seeds of commercial lots from three locations in the department of Cordoba, were used. Five trees of each specie were randomly selected and from each tree, five samples of one hundred seeds, were randomly selected. The description of the shape and the anatomy of the seeds was made according to ten seeds of each specie. For the tetrazolium tests, a completely randomized design experiment, with six treatments, and four replications of 25 seeds, was established. The six treatments were originated

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Section: Determinación De Patrones Topológicosunclassified

Section: Evaluación De Diferentes Concentraciones De Tetrazolio Y Tieunclassified

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Características morfométricas, anatómicas y viabilidad de semillas de Cedrela odorata L. y Cariniana pyriformis Miers*.

Camacho

Tatis²,

Ayala³

2017

Mesoam. Agron.

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“…The descriptions found in the mentioned references showed efficiencies comparable to germination and vigor tests, such as, Barros et al (2005), testing zucchini seeds, Bhering et al (2005) and Nery et al (2007) testing watermelon seeds, Lima et al (2007) with melon seeds, Santos et al (2007) testing tomato, Lima et al (2010) evaluating cucumber and Gagliardi & Marcos Filho (2011) testing green pepper seeds.…”

Section: Palavras-chavementioning

confidence: 90%

Comparing procedures for performing tetrazolium test on carrot seeds

2018

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The tetrazolium (TZ) test is one of the main methods to estimate vitality or viability and seed vigor. The aim of this study was to improve the methodology and reduce the execution time of tetrazolium test on carrot seeds, considering two existing references on this subject. Eight lots of 'Brasilia' carrot seeds were used. The hydration, during the pre-conditioning of the seeds, was done in two ways: directly in water during 18 hours and in rolls of filter paper during 2 hours. Seed color was analyzed through combinations between cutting types, concentration, period and temperature used during contact with the TZ solution. Three types of longitudinal cutting were used, before immersion in the tetrazolium solution: a) lateral and as distant as possible from the embryo distal to the embryo; b) partial, in the distal region opposite to the embryo, on about 1/3 of seed length; c) lateral and near the embryo, without reaching it. The used TZ concentrations were 0.1; 0.5 and 1.0%; periods of contact of the seeds with TZ solution were 1, 2, 6 and 24 h and temperatures were 30 and 35°C. The experimental design was completely randomized, with 5 replicates of 20 seeds per lot, per procedure. The results obtained through TZ test were compared with the results obtained in germination and seedling emergence tests. We could make hydration period shorter, from 18 to 2 hours, and staining from 24 to 2 hours. The lateral cutting as close as possible to the embryo, without reaching it, used in preparing the staining, should be emphasized for making execution and interpretation easy. The combination of higher efficiency used for hydration rolls of filter paper during 2 hours and, for staining lateral cutting as close as possible to the embryo, without reaching it, with the development of staining during 2 hours in 0.1% TZ solution at 35ºC. Thus, the reduction of the maximum tetrazolium test time, considering hydration (18 hours) and staining (24 hours), was from 42 to 4 hours and showed to be a feasible and reliable alternative.

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“…The wheat occupies the sixth place in the rank of the most cultivated food crops worldwide (FOOD AND AGRICULTURE ORGANIZATION OF THE UNITED NATION-FAOSTAT, 2011) and constitutes the main source of calories for more than 1.5 million people (MANSKE et al, 2001). In Brazil, the annual production of this cereal reaches only 5.9 million metric tons; notwithstanding the national consumption of the grain requires 10.4 million metric tons annually, being thus enormously insufficient to supply the internal market demand (COMPANHIA NACIONAL DE ABASTECIMENTO-CONAB, 2011).…”

Section: Introductionmentioning

confidence: 99%

Improved assessment of wheat seeds vigor

et al. 2012

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The tetrazolium test is widely used for seed vigor assessment of various plant species. For wheat, however, there is no methodology specifically recommended. This study aimed at determining an efficient procedure to evaluate vigor of wheat seeds by this test; besides allowing the establishment of seed quality classes. Thus, four different wheat seed lots were assessed by determining the moisture content and through tests of germination, accelerated aging, and field seedling emergence. The following methodologies were assessed: 1) longitudinal bisection of seed, with subsequent placement of the two halves on filter paper moistened with a 1.0% tetrazolium solution, at 30º C, for 2 h; 2) longitudinal bisection, with subsequent immersion of one seed half into a 1.0% tetrazolium solution, at 30º C, for 3 h; and 3) longitudinal bisection, with subsequent immersion of one seed half into a 0.075% tetrazolium solution, at 40º C, for 2 h. It was concluded that the tetrazolium test is more efficient in evaluating wheat seed vigor when performed with immersion of one half of the seed into a 0.075% tetrazolium solution (40º C, for 2 h) or a 0.1% tetrazolium solution (30º C, during 3 h); allowing to sort seeds into four quality classes. Index terms:Triticum aestivum, physiological quality, viability. RESUMOO teste de tetrazólio é amplamente utilizado para avaliar o vigor de sementes de várias espécies. Porém, para o trigo, não há recomendação de metodologia específica. O objetivo, neste estudo, foi determinar um procedimento eficiente para avaliar o vigor de sementes de trigo, por meio deste teste, permitindo estabelecer classes de qualidade. Foram avaliados quatro diferentes lotes de sementes de trigo, por meio da determinação do grau de umidade e dos testes de germinação, envelhecimento acelerado e emergência de plântulas em campo. Foram testadas as seguintes metodologias: 1) bissecção longitudinal, com disposição das duas metades sobre papel embebido em solução de tetrazólio a 1,0%, a 30° C, por 2 h; 2) bissecção longitudinal, com imersão de uma das metades em solução de tetrazólio a 0,1%, a 30° C, por 3 h; e 3) bissecção longitudinal, com imersão de uma das metades em solução de tetrazólio a 0,075%, a 40° C, por 2 h. Concluiu-se que o teste de tetrazólio é mais eficiente quando conduzido mediante imersão de uma das metades da semente em soluções de tetrazólio a 0,075% (40° C, por 2 h) ou a 0,1% (30° C, por 3 h), permitindo a classificação das sementes em quatro classes de qualidade. Termos para indexação:Triticum aestivum, qualidade fisiológica, viabilidade.

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Avaliação da viabilidade e do vigor de sementes de pepino pelo teste de tetrazólio

Cited by 14 publications

References 6 publications

Características morfométricas, anatómicas y viabilidad de semillas de Cedrela odorata L. y Cariniana pyriformis Miers*.

Características morfométricas, anatómicas y viabilidad de semillas de Cedrela odorata L. y Cariniana pyriformis Miers*.

Comparing procedures for performing tetrazolium test on carrot seeds

Improved assessment of wheat seeds vigor

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