Padrões eletroforéticos da enzima alfa-amilase em sementes de milho submetidas a alta temperatura de secagem

José, Solange Carvalho Barrios Roveri; Pinho, Édila Vilela de Resende Von; Pinho, Renzo Garcia Von; Silveira, César Martoreli da

doi:10.1590/s0101-31222004000100012

Cited by 5 publications

(7 citation statements)

References 23 publications

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“…These authors claim that during the seed maturation phase, analysis of reserve material accumulation through molecular markers may allow assessment of seed quality, and that α-amylase activity has been used as marker for desiccation tolerance in maize seeds. This result is in agreement with that of José et al (2004b), in which isoenzymatic proiles for α-amylase showed a higher bandwidth for lineage and hybrid seeds that are tolerant to high drying temperature. Further, Andrade et al (2013) also found expression of these enzymes in in relation to heat resistance in maize seeds.…”

Section: A Lopes Et Alsupporting

confidence: 90%

“…Some research has shown that the physiological quality of maize seed (Zea mays) is inluenced not only by environmental factors, but also by the genotype and its interactions with the environment Importance of amylases in seed quality (HOECKER et al, 2006;REIS et al, 2011;OLIVEIRA et al, 2013;. Many authors such as Rood et al (1990), Causse et al (1995), Gomes et al (2000), José et al (2004b), Hoecker et al (2006 and Reis et al (2011) relate quality to the inluence of heterosis or hybrid vigor in seed germination. In several of these studies, hybrid plants were found to have more eficient enzyme systems compared to other lineages.…”

Section: Developmentmentioning

confidence: 99%

“…In addition to the physiological tests, some studies have associated the expression of enzymes (tested through electrophoresis) with the physiological quality of seeds (JOSÉ et al, 2004b;. According to Copeland and McDonald (2001), more sensitive assessments for detecting early seed deterioration can be made through evaluation of enzyme activity associated with biosynthesis of new tissue, as these enzymes become less eficient in terms of catalytic activity along the process of deterioration.…”

Section: Developmentmentioning

confidence: 99%

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Importance of amylases for physiological quality in maize seeds

et al. 2017

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ResumoImportância das amilases na qualidade isiológica de sementes de milho. A qualidade de sementes está associada à soma dos atributos genéticos, físicos, isiológicos e sanitários que afetam sua capacidade para realizar as funções vitais, estando relacionada à germinação, ao vigor e à longevidade. A expressão de genes associados à qualidade isiológica pode ser avaliada por meio de análises de germinação e vigor, e ainda pelas análises de transcritos e de proteínas em sementes. Objetivou-se com este trabalho fazer uma revisão sobre a relevância das enzimas do grupo das amilases relacionadas à qualidade isiológica de sementes de milho. Dentro do grupo das amilases, a α-amilase (1,4-α-D-glucan-glucanohydrolase, E.C 3.2.1.1) é uma enzima importante na hidrólise do amido, sendo responsável por 90% da atividade amilolítica em sementes de milho. Essa enzima, a exemplo da dextrose, causa a conversão de amido em açúcares que são utilizados no crescimento do embrião. Já a β-amilase (1,4-α-D-glucan maltohydrolase, E.C 3.2.1.2) catalisa a liberação de maltose e dextrinas a partir das extremidades não redutoras do amido. Pesquisas comprovam que as enzimas amilases estão ligadas diretamente à qualidade isiológica de sementes de milho. A α-amilase e a β-amilase estão envolvidas principalmente no processo de germinação e na heterose das sementes, podendo ser também utilizadas como marcadores moleculares relacionadas à tolerância de secagem das sementes.Palavras-chave: α-amilase; β-amilase; Germinação; Heterose; Marcador molecular AbstractSeed quality is the result of the sum of genetic, physical, physiological and sanitary attributes that affect seed ability to perform vital functions related to germination, vigor, and longevity. The expression of genes associated with physiological quality can be assessed by means of germination and vigor analyses, as well as by transcript and protein analyses. The objective in this work was to review the relevance of amylase group enzymes to the physiological quality of maize seeds. Within this group, α-amylase (1,4-α-D-glucan glucanohydrolase E.C 3.2.1.1) plays an important role in starch hydrolysis, and is responsible for 90% of the amylolytic activity in maize seeds. It is responsible for starch conversion into sugars (e.g., destrin), which is used for embryo growth. β-amylase (1,4-α-D-glucan maltohydrolase E.C 3.2.1.2) catalyzes the release of maltose and dextrins from the non-reducing ends of starch. Research has shown that amylase enzymes are directly linked to physiological quality of maize seeds. Alpha-and beta-amylases are mainly involved in the germination process and seed heterosis, and can also be used as molecular markers associated with seed tolerance for drying.

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Section: A Lopes Et Alsupporting

confidence: 90%

Section: Developmentmentioning

confidence: 99%

Section: Developmentmentioning

confidence: 99%

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Importance of amylases for physiological quality in maize seeds

et al. 2017

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“…Seeds of Pisum sativum were sensitive to temperature higher than 40 ºC (Siddique and Wright, 2003), while the ones of Jatropha curcas had the electrical conductivity increased at 60 ºC, but only at 70 ºC the germination decreased (Ullmann et al, 2010). According to José et al (2004), the tolerants lines of Zea mays seeds to high temperature had no significant differences in enzyme activity for shade-dried seeds or artificially dried seeds.…”

Section: Resultsmentioning

confidence: 99%

Viability and cell cycle of Melanoxylon brauna seeds submitted to drying and imbibition

Silva¹,

Carvalho²,

Borges³

et al. 2014

J. Seed Sci.

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-The aim of this work was to evaluate viability and DNA ploidy of seed of Melanoxylon brauna submitted or not to fast drying, along soaking. The seeds were dried followed by germination and quantification of DNA ploidy pattern. There was a gradual decrease in germination percentage with increases in temperature and drying time. The DNA ploidy of embryonic axes that were not submitted to artificial drying indicated the existence of nuclei 2C, 4C and 8C. However, 95% of the tested embrios had 2C DNA ploidy . The seeds that were subjected to rapid drying also exhibited the presence of nuclei 2C, 4C and 8C and showed no significant differences ( p > 0.05 ) compared to seeds that have not passed through drying. It is conclude that the seeds of Melanoxylon brauna submitted to different drying times and temperatures show a gradual decrease in the percentage of germination; embryonic axes subject or not to drying seeds have 2C, 4C and 8C nuclei and drying does not affect the cell cycle of cells in the embryo of the seed. Termos para indexação: ploidia, citometria de fluxo, secagem.

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“…These markers can be affected by many environmental factors and especially by stress. In contrast, heat resistant proteins are potential markers that do not present any known catalytic activity (Silva-Mann et al, 2002) presenting polymorphism and stability in seeds, such as in maize (Roveri José et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

Physiological quality and protein patterns of corn seeds produced under water and salt stresses

Diniz¹,

Pinho²,

Santos³

et al. 2015

Afr. J. Agric. Res.

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This study evaluated the effect of water and salt stresses on the physiological quality and on the electrophoresis patterns of heat resistant proteins in corn seeds. The experiments were carried out in the laboratories of seed analysis and biotechnology at the Universidade Federal de Lavras. Corn seeds of the hybrid GNZ 2004 and the inbred line LE 57 were used. The seeds were produced in soils with an electric conductivity of 3 dS m -1 (under stress) and 0.4 dS m -1 (without stress) and in pots containing substrate with a water holding capacity of 40% (with stress) and 70% (without stress). The randomized complete block design with treatments arranged in a split-plot scheme was used with four replications. Seeds were harvested at different stages of development. Seed physiological quality was evaluated using the tests of germination, artificial aging and cold test. The patterns of heat resistant proteins were evaluated by electrophoresis. The results showed that depending on seed developmental stage there was an effect of water and salt stresses on the seed vigor. The electrophoretical patterns of heat resistant proteins were stable in seeds produced under different stress conditions.

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Padrões eletroforéticos da enzima alfa-amilase em sementes de milho submetidas a alta temperatura de secagem

Cited by 5 publications

References 23 publications

Importance of amylases for physiological quality in maize seeds

Importance of amylases for physiological quality in maize seeds

Viability and cell cycle of Melanoxylon brauna seeds submitted to drying and imbibition

Physiological quality and protein patterns of corn seeds produced under water and salt stresses

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