Detection of genetically modified maize events in Brazilian maize-derived food products

Branquinho, Maria Regina; Gomes, Déborah Márcia Vasconcelos; Ferreira, Renata Trotta Barroso; Lawson-Ferreira, Rafael; Cardarelli-Leite, Paola

doi:10.1590/s0101-20612013005000063

Cited by 6 publications

(4 citation statements)

References 16 publications

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“…A summary of electrophoresis results is displayed in Figures 4 and 5. This observation is consistent with [35] Arun et al (2013), showed that among 14 tested samples containing maize ingredients, 8 contained Bt11 maize transgenic variety. [12] Similarly, previous study conducted in Iran proved that of all 5 non-labeled positive maize samples, 2 and 3 containing GM lines, that is CaMV35 S promoter were Bt11 and MON810, respectively.…”

Section: Quality Assurancesupporting

confidence: 91%

Qualitative PCR-based detection of genetically modified soy and maize products in Iran

Safaei

Rezaie

Alimohammadi

et al. 2020

International Journal of Food Properties

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Soy and maize are the most cultivated genetically modified (GM) crops in the world, which are commonly used in many food products. Establishing regulations for food products containing transgenic materials are obligatory in many countries in order to provide consumers with information. For this purpose, an accurate qualitative polymerase chain reaction (PCR) method was applied to investigate GM food products from Iran. In present study, a total of 90 non-labeled soy and maize samples were collected from Tehran's market and their DNA were extracted by a kit. All samples were analyzed to screen the 35 S promoter and (nopaline synthase) NOS terminator elements. Having screened the GM positive samples, they were subjected to identification of specific transgenic events RR soy, Bt11 and MON810 maize with PCR. According to our results, 95% of soy and 60% of maize analyzed samples were positive for the 35 S promoter and NOS terminator. Furthermore, event-specific analysis indicated the presence of RR soy, Bt11 and MON810 maize in tested samples, while none of these food-derived samples demonstrated any GM label. The obtained results revealed that the necessity of monitoring system to provide a good reliable control of GM materials in food products and subsequently on their labeling.

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Section: Quality Assurancesupporting

confidence: 91%

Qualitative PCR-based detection of genetically modified soy and maize products in Iran

Safaei

Rezaie

Alimohammadi

et al. 2020

International Journal of Food Properties

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“…Although requiring special reagents and equipment, PCR can detect the presence of specific exogenous genes inserted into the plant genome (including the seeds), and represents the most widely used method to detect GM food and feed (Dinon et al, 2011;Branquinho et al, 2013;Cantelmo et al, 2013). In the present study, we used real time PCR to detect three GM-specific fragments: the promoter region p-35S derived from the cauliflower mosaic virus (Waiblinger et al, 2008;Huber et al, 2013), the terminator region, t-Nos, derived from the nopaline synthase gene of Agrobacterium tumefaciens (Reiting et al, 2007;Huber et al, 2013), and the main cry1A.105 gene from Bt (Dinon et al, 2011).…”

Section: Discussionmentioning

confidence: 99%

“…The only non-labeled flour was negative for the three GM targets. A study was previously performed to verify compliance with Brazilian laws on the reporting of GM crops in food (Branquinho et al, 2013). Those authors reported the occurrence of several food products with GM organisms that were not properly labeled between 2011 and 2012.…”

Section: Discussionmentioning

confidence: 99%

Detection of genetically modified maize in processed products, dry grains, and corn ears intended for fresh consumption in South Brazil

et al. 2016

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ABSTRACT. Conventional and genetically modified (GM) maize cultivars have been widely planted in Brazil to produce grains for processed food, feed, or to be consumed fresh as corn ears. This study used real-time PCR to detect GM maize in processed products and fresh commercial corn ears produced in the last two years in South Brazil. Eighteen conventional and GM maize cultivars were obtained from seed production companies and 50 commercial samples (including canned corn, corn flour, dry grains, and fresh corn ears) were purchased in small local stores and supermarkets. All samples were analyzed by real time TaqMan PCR to detect one constitutive maize gene (hmg) and three genetic regions present in GM plants (p-35S promoter, major gene cry 1A.105, and t-Nos terminator). Each commercial sample was classified as conventional or GM based on the PCR results. PCR targeting the 2 C.A.M. Oliveira et al. Genetics and Molecular Research 15 (4): gmr15048818hmg gene generated positive results from all DNA samples, which were further tested with the GM targets. These targets were not detected in the five conventional maize cultivars, but were detected in the GM seeds hosting these fragments. Analysis of processed foods identified four cultivars as conventional and six as GM, which were mostly correctly labeled. Seven (53.8%) dry grain samples were classified as conventional, while six (46.2%) were classified as GM. Three (11.1%) corn ear samples were identified as conventional, and the remaining 24 (88.9%) were GM maize. These results demonstrate the high frequency of GM maize in processed products, including fresh corn ears intended for consumption in South Brazil.

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“…A rotulagem é um instrumento com múltiplas funções: (i) permite ao consumidor o direito de ser informado, de maneira adequada, sobre a composição do alimento que está disposto a consumir, o que possibilita escolhas e decisões informadas, (ii) atende a necessidade do consumidor que pertence a um grupo de risco de evitar consumir produtos que não deveria por prescrição médica, e (iii) garante a segurança do consumidor ao permitir o rastreamento e o controle pós-comercialização de produtos alimentícios, possibilitando o controle de qualidade destes produtos 26 e pesquisas sobre os impactos na saúde. No entanto, muitos alimentos contendo ingredientes derivados de variedades transgênicas não têm sua composição claramente identificada no rótulo [27][28][29][30] .…”

Section: Introductionunclassified

Reflexões sobre a proposta de modificação da regulamentação de rotulagem de alimentos transgênicos no Brasil

Cortese

Martinelli

Fabri

et al. 2021

Ciênc. saúde coletiva

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Resumo Diante da existência de incertezas científicas em relação à segurança dos transgênicos para a saúde humana e considerando o Princípio da Precaução e preceitos constitucionais em vigor, o consumidor deve ter o direito de ser informado de maneira adequada sobre a presença de transgênicos nos alimentos, por meio da rotulagem. Este ensaio tem por objetivo apresentar as implicações acerca da nova proposta de rotulagem de transgênicos no Brasil. A atual legislação brasileira de rotulagem de alimentos transgênicos e agências governamentais envolvidas não garantem que os produtos não identificados como tal sejam livres de transgênicos. A aprovação do PLC nº 34/2015 contraria dispositivos do Código de Defesa do Consumidor, indo na contramão da escolha e autonomia do consumidor. Além disso, a biovigilância será mais ainda inepta a executar uma atividade de identificação e apreensão de produtos que venham causar danos à saúde humana, animal e ao meio ambiente. A mudança proposta representa um retrocesso na regulamentação de rotulagem de transgênicos vigente no Brasil e um desrespeito aos direitos individuais e coletivos previstos na Constituição Federal, no Código de Defesa do Consumidor e em acordos internacionais assinados pelo Brasil.

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Detection of genetically modified maize events in Brazilian maize-derived food products

Cited by 6 publications

References 16 publications

Qualitative PCR-based detection of genetically modified soy and maize products in Iran

Qualitative PCR-based detection of genetically modified soy and maize products in Iran

Detection of genetically modified maize in processed products, dry grains, and corn ears intended for fresh consumption in South Brazil

Reflexões sobre a proposta de modificação da regulamentação de rotulagem de alimentos transgênicos no Brasil

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