Extraction of DNA from honey and its amplification by PCR for botanical identification

Jain, Sona; Jesus, Flávia Thalita de; Marchioro, Giulia Manso; Araújo, Edilson Divino de

doi:10.1590/s0101-20612013000400022

Cited by 36 publications

(26 citation statements)

References 19 publications

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“…dna extraction and Pcr Propolis (5 g) was washed with hexane. Then, 200 mg of each pre-washed sample was used for DNA extraction using a CTAB method as described by Jain et al (2013Jain et al ( , 2014. The extracted DNA was dissolved in 25 μL of TE and stored at -20ºC for further use.…”

Section: Ultra-fast Liquid Chromatography (Uflc)mentioning

confidence: 99%

Botanical Origin of the Brazilian Red Propolis: a New Approach Using DNA analysis

Jain

Marchioro

Mendonça

et al. 2014

Journal of Apicultural Science

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a b s t r a c t Propolis is produced by the honeybees by using resin and other plant secretions. Propolis from different geographical regions have different chemical compositions. this is because the chemical constituents of propolis depend on the vegetation surrounding the apiary. in this report we present a new approach using dna barcoding for the identification of the botanical origin of propolis. red propolis samples were collected at different times of the year from the state of sergipe situated in northeast Brazil. extraction of the dna from propolis was made using a ctaB method. amplification was done using its2 universal primers, followed by dna sequencing. sequence analysis confirmed the presence of Dalbergia ecastaphyllum in the Brazilian red propolis. formononetin is a chemical marker for the Brazillian red propolis and D. ecastaphyllum. Propolis samples analysed by dna sequencing, were also checked by Ultra-fast liquid chromatography for the presence of formononetin. Peaks corresponding to formononetin were observed in all the analysed propolis samples. this is the first report of the botanical origin of propolis using dna technology.

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Section: Ultra-fast Liquid Chromatography (Uflc)mentioning

confidence: 99%

Botanical Origin of the Brazilian Red Propolis: a New Approach Using DNA analysis

Jain

Marchioro

Mendonça

et al. 2014

Journal of Apicultural Science

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“…While plant identification from DNA has presented numerous challenges (CBOL 2009), it has the advantage of providing finer definition of species‐specific foraging preferences for plant families whose pollen is difficult to distinguish morphologically (Bell et al., 2016; Bruni et al., 2015; Hawkins et al., 2015; Kraaijeveld et al., 2015; Pornon, Andalo, Burrus, & Escaravage, 2017). DNA sequencing has been used successfully to identify the foraging preference of honeybees and the floral composition of their honey (Bruni et al., 2015; De Vere et al., 2017; Galimberti et al., 2014; Hawkins et al., 2015; Jain, Jesus, Marchioro, & Araújo, 2013; Valentini, Miquel, & Taberlet, 2010). Several studies have also used pollen DNA sequencing to identify solitary bee foraging preferences (Sickel et al., 2015; Wilson, Sidhu, Levan, & Holway, 2010).…”

Section: Introductionmentioning

confidence: 99%

Flower preferences and pollen transport networks for cavity‐nesting solitary bees: Implications for the design of agri‐environment schemes

Gresty

Clare

Devey

et al. 2018

Ecology and Evolution

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Floral foraging resources are valuable for pollinator conservation on farmland, and their provision is encouraged by agri‐environment schemes in many countries. Across Europe, wildflower seed mixtures are widely sown on farmland to encourage pollinators, but the extent to which key pollinator groups such as solitary bees exploit and benefit from these resources is unclear. We used high‐throughput sequencing of 164 pollen samples extracted from the brood cells of six common cavity‐nesting solitary bee species (Osmia bicornis, Osmia caerulescens, Megachile versicolor, Megachile ligniseca, Megachile centuncularis and Hylaeus confusus) which are widely distributed across the UK and Europe. We documented their pollen use across 19 farms in southern England, UK, revealing their forage plants and examining the structure of their pollen transport networks. Of the 32 plant species included currently in sown wildflower mixes, 15 were recorded as present within close foraging range of the bees on the study farms, but only Ranunculus acris L. was identified within the pollen samples. Rosa canina L. was the most commonly found of the 23 plant species identified in the pollen samples, suggesting that, in addition to providing a nesting resource for Megachile leafcutter bees, it may be an important forage plant for these species. Higher levels of connectance and nestedness were characteristic of pollen transport networks on farms with abundant floral resources, which may increase resilience to species loss. Our data suggest that plant species promoted currently by agri‐environment schemes are not optimal for solitary bee foraging. If a diverse community of pollinators is to be supported on UK and European farmland, additional species such as R. canina should be encouraged to meet the foraging requirements of solitary bees.

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“…Species-specific real-time PCR assays with TaqMan™ probes were successfully developed to detect relevant species in Corsican honey (acacia, broom, citrus, clover, heather, eucalyptus, lavender, linden, oak, olive, rape, rockrose, rosemary, sunflower, and sweet chestnut) (Laube et al, 2010). However, previous sample pre-treatments and the selection/optimisation of DNA extraction from honey are crucial steps for further amplification by polymerase chain reaction (PCR) (Jain, Jesus, Marchioro, & Araújo, 2013;Lalhmangaihi, Ghatak, Laha, Gurusubramanian, & Kumar, 2014;Soares, Amaral, Oliveira, & Mafra, 2015).…”

Section: Introductionmentioning

confidence: 99%

Botanical authentication of lavender (Lavandula spp.) honey by a novel DNA-barcoding approach coupled to high resolution melting analysis

et al. 2018

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a b s t r a c tMonofloral honeys (such as, lavender honey) are highly appreciated by the consumers due to their flavour, taste and properties. However, since they are considered prime products, they are often targets of adulteration. This work exploits DNA barcoding combined with high resolution melting (HRM) analysis to establish the botanical origin of honey, using lavender honey as a case study. The plastidial matK gene was targeted as a candidate barcode for Lavandula species identification. The method allowed differentiating the species in three clusters with confidence levels >99%, being the results well correlated with the sequencing analysis. It was successfully applied to identify the botanical origin of several lavender honeys, which were grouped in the cluster of the most common species in Portugal (L. stoechas subsp., L. penduculata and L. viridis). The proposed method represents a simple, specific and cost-effective tool to authenticate the botanical origin of honey.

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Extraction of DNA from honey and its amplification by PCR for botanical identification

Cited by 36 publications

References 19 publications

Botanical Origin of the Brazilian Red Propolis: a New Approach Using DNA analysis

Botanical Origin of the Brazilian Red Propolis: a New Approach Using DNA analysis

Flower preferences and pollen transport networks for cavity‐nesting solitary bees: Implications for the design of agri‐environment schemes

Botanical authentication of lavender (Lavandula spp.) honey by a novel DNA-barcoding approach coupled to high resolution melting analysis

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