Botanical authentication of lavender (Lavandula spp.) honey by a novel DNA-barcoding approach coupled to high resolution melting analysis

Soares, Sónia; Grazina, Liliana; Costa, Joana; Amaral, Joana S.; Oliveira, M. Beatriz P.P.; Mafra, Isabel

doi:10.1016/j.foodcont.2017.11.046

Cited by 41 publications

(30 citation statements)

References 24 publications

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“…DNA barcoding, usually mini-barcoding, can be combined with high resolution melting (HRM) for the authentication of many commercial species from fake products such as Gadidae fish [ 69 ], Monofloral honeys [ 70 ] as well as herbal medicines from toxic species, such as Crotalaria spectabilis Roth. in Thunbergia laurifolia Lindl.…”

Section: Fluorescence Methods Of Dna Barcodingmentioning

confidence: 99%

Advances in DNA Barcoding of Toxic Marine Organisms

Gong¹,

Ding²,

Wang³

et al. 2018

IJMS

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There are more than 200,000 marine species worldwide. These include many important economic species, such as large yellow croaker, ribbonfish, tuna, and salmon, but also many potentially toxic species, such as blue-green algae, diatoms, cnidarians, ctenophores, Nassarius spp., and pufferfish. However, some edible and toxic species may look similar, and the correct identification of marine species is thus a major issue. The failure of traditional classification methods in certain species has promoted the use of DNA barcoding, which uses short, standard DNA fragments to assist with species identification. In this review, we summarize recent advances in DNA barcoding of toxic marine species such as jellyfish and pufferfish, using genes including cytochrome oxidase I gene (COI), cytochrome b gene (cytb), 16S rDNA, internal transcribed spacer (ITS), and Ribulose-1,5-bisphosphate carboxylase oxygenase gene (rbcL). We also discuss the application of this technique for improving the identification of marine species. The use of DNA barcoding can benefit the studies of biological diversity, biogeography, food safety, and the detection of both invasive and new species. However, the technique has limitations, particularly for the analysis of complex objects and the selection of standard DNA barcodes. The development of high-throughput methods may offer solutions to some of these issues.

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Section: Fluorescence Methods Of Dna Barcodingmentioning

confidence: 99%

Advances in DNA Barcoding of Toxic Marine Organisms

Gong¹,

Ding²,

Wang³

et al. 2018

IJMS

View full text Add to dashboard Cite

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“…DNA barcoding is a newly discovered approach to species identification using a standard region of DNA that is widely employed in the large‐scale identification of animal species . DNA barcoding has also been applied in taxonomic identification of flowering plants . Several DNA barcode regions, such as internal transcriber spacers ( ITS1 and ITS2 ) and plastidial genes psbA‐trnH , trnL‐F , matK , and rbcL , have become the most promising loci in plant differentiation .…”

Section: Introductionmentioning

confidence: 99%

“…17 DNA barcoding has also been applied in taxonomic identification of flowering plants. [18][19][20] Several DNA barcode regions, such as internal transcriber spacers (ITS1 and ITS2) and plastidial genes psbA-trnH, trnL-F, matK, and rbcL, have become the most promising loci in plant differentiation. 21 The psbA-trnH sequence has a faster evolution rate and higher species-identification efficiency, and it is becoming a commonly used and reliable chloroplast barcode gene in the study of plant molecular phylogeny.…”

Section: Introductionmentioning

confidence: 99%

DNA barcoding coupled with high‐resolution melting analysis for nut species and walnut milk beverage authentication

et al. 2020

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Background Walnut (Juglans regia L.) is one of the most widely cultivated nuts. Walnut milk beverage is very popular in China due to its nutritional value. However, adulterated walnut milk ingredients have been detected in the Chinese market. Peanut and soybean are sold at much lower prices than walnut and are reported to be commonly used for adulteration in the industrial chain of walnut milk production. The purpose of this study is therefore to develop an accurate and efficient method for detecting the authenticity of the raw materials used in walnut milk beverage. Results DNA barcoding and high‐resolution melting (HRM) analyses were used to identify common adulterated raw ingredients such as peanut and soybean in commercial walnut milk beverage samples. The chloroplast psbA‐trnH gene was used for sequencing, and HRM analysis was performed. We also prepared experimental mixtures, in the laboratory, with different quantities of walnut, peanut, and soybean. High‐resolution melting analysis of the experimental mixtures clearly distinguished all of them. The results revealed that most of the walnut milk beverage samples fell in the same cluster of walnut species. Several samples fell in the peanut cluster, confirming that they were adulterated products. Conclusion The results revealed that HRM analysis based on the psbA‐trnH barcode sequence can be used to identify raw ingredients in walnut milk beverages. © 2020 Society of Chemical Industry

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“…The trnL region discriminated 13 species from the Leguminaceae family (Madesis, Ganopoulos, Anagnostis, & Tsaftaris, 2012) and five species used for fruit juice (Faria, Magalhães, Nunes, & Oliveira, 2013). The matK region was used to discriminate five species of Crocus sativus (Villa, Costa, Meira, Oliveira, & Mafra, 2016) and three different Lavandula species in lavender honey (Soares et al, 2018). A combination of the rbcL and trnL regions (two individual simplex reactions) was used to identify eight broomrape species (Rolland, Dupuy, Pelleray, & Delavault, 2016).…”

Section: Introductionmentioning

confidence: 99%

High-resolution melting of multiple barcode amplicons for plant species authentication

et al. 2019

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In recent years, species identification in herbs has attracted considerable attention due to several cases of fraud; hence inexpensive high-throughput authentication methods are highly welcomed. Species authentication is often performed through DNA analysis and several specific regions (barcodes) are considered suitable. Each barcode (Bar) possesses different qualities in terms of universality and discrimination power. A multiplexed format where information can be extracted simultaneously from several barcode regions is seemingly appropriate to ensure the power of both universality and discrimination. In this approach, we amplified DNA from five different barcode regions in a multiplexed PCR format followed by high-resolution melting (HRM). This multiplexed Bar-HRM approach was first applied to plants spanning the plant kingdom and then gradually narrowing down the genetic variability within the Lamiaceae and the Solanaceae families to finally reach closely related cultivars. Universality was demonstrated through distinct melting profiles obtained for species originating from 29 different families spanning the angiosperms, gymnosperm, mosses, and liverwort (Marchantiophyta). Discrimination power was retained for species, sub-species, and a few cultivars through the application of multivariate statistics to the high-resolution melting profiles. This preliminary investigation has shown the potential to discriminate a vast amount of species within the whole plant kingdom. It requires no a priori knowledge of the species' DNA sequence and occurs in a closed system within 2.5 h at a reduced cost per sample compared to other DNA based approaches.

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Botanical authentication of lavender (Lavandula spp.) honey by a novel DNA-barcoding approach coupled to high resolution melting analysis

Cited by 41 publications

References 24 publications

Advances in DNA Barcoding of Toxic Marine Organisms

Advances in DNA Barcoding of Toxic Marine Organisms

DNA barcoding coupled with high‐resolution melting analysis for nut species and walnut milk beverage authentication

High-resolution melting of multiple barcode amplicons for plant species authentication

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