Lipase and esterase: to what extent can this classification be applied accurately?

Molecular and enzymatic characterization of alkaline lipase from Bacillus amyloliquefaciens E1PA isolated from lipid-rich food waste

“…However, lipases are generally produced using lipidic carbon sources such as oils, fatty acids and glycerol [23] Two groups of hydrolases are lipases (EC 3.1.1.3) and esterases (EC 3.1.1.1), and these enzymes are differentiated according to their substrate specificity [24]. Lipases primarily act on water-insoluble substrates, such as triglycerides composed of long-chain fatty acids, whereas esterases preferentially hydrolyze 'simple' esters and typically act on only triglycerides composed of fatty acid chains shorter than C6 [25]. Lipase is an important enzyme that is used in biotechnological applications.…”

Biochemical characterisation and application of lipases produced by Aspergillus sp. on solid‐state fermentation using three substrates

“…() and Lopes et al . (). Enzyme aliquot (0.070 mL) was added to 3.43 mL of reaction mixture containing 1.12 m m pNPB in 50 m m phosphate buffer pH 7.2 added of 0.2% (w/v) Triton X‐100 and 0.43 m tetrahydrofuran.…”

“…() and Lopes et al . (). The reaction mixture containing 0.9 mL substrate solution, 0.1 mL 50 m m phosphate buffer and 0.1 mL of enzyme was incubated at 37 °C for 30 min.…”

Chemical and Biological Investigation of Organic Wastes of Frying Oils and Beef Fats: Valorization for Biodiesel Production

“…During fungi cultivation, and at regular 24 h intervals, samples were withdrawn and assayed for lipase activity. It was measured according to the method of Lopes et al (2011) [31], using the quantitative assay by titration of the liberated fatty acids after contact between enzymes and the substrate (triglyceride emulsion). Liberated fatty acids were immediately titrated by NaOH solution (0.05 M).…”