Estudo da produção de biopolímeros via enzimática através de inativação e lise celular e com células viáveis de Beijerinckia sp. 7070

Moreira, Ângela Nunes; Pino, Francisco A. B. Del; Vendruscolo, Claire Tondo

doi:10.1590/s0101-20612003000200034

Cited by 11 publications

(10 citation statements)

References 10 publications

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“…They may also be recovered by the process of precipitation after treatment with organic solvents soluble in water. The most commonly used are ethanol, acetone, methanol, and isopropanol (CERNING et al, 1994;MOREIRA;PINO;VENDRUSCOLO, 2003;GÓMEZ, 2006;BENNAMA et al, 2012). Extracellular microbial biopolymers have been widely used in several industry sectors adding value to various products, especially those food products, to improve their rheological characteristics.…”

Section: Resultsmentioning

confidence: 99%

An alternative method for screening lactic acid bacteria for the production of exopolysaccharides with rapid confirmation

et al. 2012

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The accumulation of exopolysaccharides (EPS) produced by microorganisms occurs in the presence of excess substrate and limiting conditions of elements that are essential to growth, such as nitrogen, phosphorus, sulfur, and magnesium. The presence of EPS produced by bacterial cells contributes to slime colonies formation in solid medium and increased viscosity in liquid medium. This paper proposes an alternative method for screening EPS-producing lactic acid bacteria using solid medium-containing discs of filter paper that are saturated with active cultures. The screening was carried out under different culture conditions varying the type of sugar, pH, and temperature. EPS production was visualized by the presence of mucoid colonies on the discs, which was confirmed by the formation of a precipitate when part of this colony was mixed with absolute alcohol. The established conditions for obtaining a high number of isolates producing EPS were 10% sucrose, pH 7.5 and 28 ºC. This method proved to be effective and economical because several strains could be tested on the same plate, with immediate confirmation.

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Section: Resultsmentioning

confidence: 99%

An alternative method for screening lactic acid bacteria for the production of exopolysaccharides with rapid confirmation

et al. 2012

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“…Studies on commercial EPS applications are more advanced for the free-living NFB, e.g., clairana produced by Beijerinckia sp. (Moreira et al, 2003) and the alginate produced by Azotobacter vinelandii (Garcia-Cruz et al, 2008).…”

Section: Possible Industrial Applications Of Rhizobium Epsmentioning

confidence: 99%

Exopolysaccharides produced by the symbiotic nitrogen-fixing bacteria of leguminosae

Bomfeti

Florentino

Guimarães³

et al. 2011

Rev. Bras. Ciênc. Solo

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SUMMARYThe process of biological nitrogen fixation (BNF), performed by symbiotic nitrogen fixing bacteria with legume species, commonly known as α and β rhizobia, provides high sustainability for the ecosystems. Its management as a biotechnology is well succeeded for improving crop yields. A remarkable example of this success is the inoculation of Brazilian soybeans with Bradyrhizobium strains. Rhizobia produce a wide diversity of chemical structures of exopolysaccharides (EPS). Although the role of EPS is relatively well studied in the process of BNF, their economic and environmental potential is not yet explored. These EPS are mostly species-specific heteropolysaccharides, which can vary according to the composition of sugars, their linkages in a single subunit, the repeating unit size and the degree of polymerization. Studies have showed that the EPS produced by rhizobia play an important role in the invasion process, infection threads formation, bacteroid and nodule development and plant defense response. These EPS also confer protection to these bacteria when exposed to environmental stresses. In general, strains of rhizobia that produce greater amounts of EPS are more tolerant to adverse conditions when compared with strains that produce less. Moreover, it is known that the EPS produced by microorganisms are widely used in various industrial activities. These compounds, also called biopolymers, provide a valid alternative for the commonly used in food industry through the development of products with identical properties or with better rheological characteristics, which can be used for new applications. The microbial EPS are also able to increase the adhesion of soil particles favoring the mechanical stability of aggregates, increasing levels of water retention and air flows in this environment. Due to the importance of EPS, in this review we discuss the role of these compounds in the process of BNF, in the adaptation of rhizobia to environmental stresses and in the process of soil aggregation. The possible applications of these biopolymers in industry are also discussed.Index terms: Exopolysaccharides, nodules, environmental stress, soil aggregation, rhizobia. RESUMO: EXOPOLISSACARÍDEOS PRODUZIDOS POR BACTÉRIAS FIXADORAS DE NITROGÊNIO SIMBIÓTICAS DE LEGUMINOSAE O processo de fixação biológica de nitrogênio (FBN), realizado por bactérias fixadoras de

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“…Quando o caldo é tratado sob condições apropriadas (80 -130°C, 10 -20 min, pH 6,3 -6,9), a dissolução da goma xantana ocorre sem degradação térmica, e o rompimento das células é observado (Smith e Pace, 1982). O aumento da temperatura também diminui a viscosidade do meio, facilitando a remoção dos insolúveis por centrifugação (Giavasis et al, 2000;Moreira et al, 2003;Boza et al, 2004) ou filtração (Giavasis et al, 2000).…”

Section: Recuperação Da Gomaunclassified

“…A precipitação do polímero é realizada por diminuição da solubilidade do colóide dissolvido, usandose métodos tais como adição de sais, de solventes miscíveis em água e concentração por evaporação, além da precipitação com solventes orgânicos, tais como o etanol e o isopropanol, o uso de misturas de álcool e sais (Giavasis et al, 2000;Moreira et al, 2003;Boza et al, 2004) e da precipitação com sais trivalentes e tetravalentes (Kennedy e Bradshaw, 1984). Também o uso de ultrafiltração tem sido relatado (Lo et al, 1997).…”

Section: Recuperação Da Gomaunclassified

Goma xantana: produção, recuperação, propriedades e aplicação

Luvielmo¹,

Scamparini²

2009

ETE

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Resumo AbstractAs bactérias pertencentes ao gênero Xanthomonas podem produzir goma xantana, um polissacarídeo de enorme interesse para as indústrias de alimentos, farmacêuticas e de petróleo. A goma apresenta capacidade de formar soluções viscosas e géis hidrossolúveis que lhe fornece propriedades reológicas únicas. Atualmente toda a goma xantana consumida no Brasil, provém de importações, porém o Brasil tem um grande potencial para a fabricação deste polímero em escala industrial, já que temos matéria-prima básica para sua produção: açúcar, extrato de levedura e álcool do setor sucro-alcooleiro. O objetivo dessa revisão é atualizar os conhecimentos sobre a produção, recuperação, propriedades e aplicação da goma xantana.The bacteria belonging to the gender Xanthomonas can produce xanthan gum, a polysaccharide of enormous interest for food, pharmaceutical and petroleum industries. The gum presents capacity to form viscous solutions and gels hydrosoluble that supplies its unique reological properties. Actually, all xanthan gum consumed in Brazil, becomes from imports, however Brazil has a great potential for production of this polymeric in industrial scale, since we have basic raw material for its production: sugar, yeast extract and alcohol of sugar-alcohol section. The objective of this review is to update the knowledge about production, recovery, properties and application of the xanthan gum.Palavras-chave: goma xantana, produção, aplicação.

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Estudo da produção de biopolímeros via enzimática através de inativação e lise celular e com células viáveis de Beijerinckia sp. 7070

Cited by 11 publications

References 10 publications

An alternative method for screening lactic acid bacteria for the production of exopolysaccharides with rapid confirmation

An alternative method for screening lactic acid bacteria for the production of exopolysaccharides with rapid confirmation

Exopolysaccharides produced by the symbiotic nitrogen-fixing bacteria of leguminosae

Goma xantana: produção, recuperação, propriedades e aplicação

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