In vitro anti-inflammatory, cytotoxic and antioxidant activities of boesenbergin A, a chalcone isolated from Boesenbergia rotunda (L.) (fingerroot)

Abstract

The current in vitro study was designed to investigate the anti-inflammatory, cytotoxic and antioxidant activities of boesenbergin A (BA), a chalcone derivative of known structure isolated from Boesenbergia rotunda. Human hepatocellular carcinoma (HepG2), colon adenocarcinoma (HT-29), non-small cell lung cancer (A549), prostate adenocarcinoma (PC3), and normal hepatic cells (WRL-68) were used to evaluate the cytotoxicity of BA using the MTT assay. The antioxidant activity of BA was assessed by the ORAC assay and compared to quercetin as a standard reference antioxidant. ORAC results are reported as the equivalent concentration of Trolox that produces the same level of antioxidant activity as the sample tested at 20 µg/mL. The toxic effect of BA on different cell types, reported as IC50, yielded 20.22 ± 3.15, 10.69 ± 2.64, 20.31 ± 1.34, 94.10 ± 1.19, and 9.324 ± 0.24 µg/mL for A549, PC3, HepG2, HT-29, and WRL-68, respectively. BA displayed considerable antioxidant activity, when the results of ORAC assay were reported as Trolox equivalents. BA (20 µg/mL) and quercetin (5 µg/mL) were equivalent to a Trolox concentration of 11.91 ± 0.23 and 160.32 ± 2.75 µM, respectively. Moreover, the anti-inflammatory activity of BA was significant at 12.5 to 50 µg/mL and without any significant cytotoxicity for the murine macrophage cell line RAW 264.7 at 50 µg/mL. The significant biological activities observed in this study indicated that BA may be one of the agents responsible for the reported biological activities of B. rotunda crude extract.

Boesenbergia rotunda

“…Boesenbergin A, from B. rotunda, exhibited cytotoxicity on non-small cell lung cancer (A549), prostate adenocarcinoma (PC3), human hepatocellular carcinoma (HepG2), colon adenocarcinoma (HT-29) and normal hepatic cells (WRL-68) with IC 50 of 20. 22, 10.69, 20.31, 94.10 and 9.324 μg/mL (Isa et al 2012 ).…”

“…B. rotunda ethanolic extract exhibited antioxidant activity as evaluated by the ferric reducing/antioxidant power (FRAP) and DPPH free radical scavenging activity (Jitvaropas et al 2012 ). Boesenbergin A, from B. rotunda, exhibited considerable antioxidant activity, when the results of ORAC assay were reported as Trolox equivalents (Isa et al 2012 ). Boesenbergin A (20 μg/mL) and quercetin (5 μg/mL) were equivalent to a Trolox concentration of 11.91 and 160.32 μM.…”

“…Panduratin A and hydroxypanduratin A showed strong activity against prostaglandin E 2 (PGE 2 ) with IC 50 values of 10.5 and 12.3 μM, respectively, and a moderate effect on tumour necrosis factor-α (TNF-α) production (IC 50 = 60.3 and 57.3 μM), respectively. Boesenbergin A exhibited signifi cant antiinfl ammatory at 12.5 to 50 μg/mL and without any signifi cant cytotoxicity for the murine macrophage cell line RAW 264.7 at 50 μg/mL (Isa et al 2012 ). Study by Boonjaraspinyo et al ( 2010 ) found that fi ngerroot clearly reduced the infl ammatory cells in hamsters that were administered N-nitrosodimethylamine (NDMA) but not in the case of liver fl uke Opisthorchis viverrini infection.…”

Thailands traditionelle Medizin: Ein Überblick

“…So soll der Schwarze Fingerwurz die Immunität stimulieren, Entzündungen bekämpfen und kanzerogenen Entwicklungen vorbeugen [15]. Immerhin scheint das Rhizom bei Magen- und Darmbeschwerden, Blähungen und Darmpilzen gute Dienste zu leisten.…”

Chemical- and Cell-based Antioxidant Capacity of Methanolic Extracts of Three Commonly Edible Plants from Zingiberaceae Family

“…13 ORAC assay has also been tested on the rhizomes which reported high antioxidant activity. 14 In 2004, Apak et al successfully employed cupric ion reducing antioxidant capacity (CUPRAC) chromophore called the Cu(I)-neocuproine (Nc) chelate which formed when undergoing redox reaction with antioxidant to evaluate antiradical activity in plant extracts. This electron-transfer based assay measures the capacity of antioxidant in reduction of an oxidant which is substantiated by colour change.…”