Influence of eNOS gene polymorphism on cardiometabolic parameters in response to physical training in postmenopausal women

Abstract

The health-promoting effects of exercise training (ET) are related to nitric oxide (NO) production and/or its bioavailability. The objective of this study was to determine whether single nucleotide polymorphism of the endothelial NO synthase (eNOS) gene at positions -786T>C, G894T (Glu298Asp) and at the variable number of tandem repeat (VNTR) Intron 4b/a would interfere with the cardiometabolic responses of postmenopausal women submitted to physical training. Forty-nine postmenopausal women were trained in sessions of 30-40 min, 3 days a week for 8 weeks. Genotypes, oxidative stress status and cardiometabolic parameters were then evaluated in a double-blind design. Both systolic and diastolic blood pressure values were significantly reduced after ET, which was genotype-independent. However, women without eNOS gene polymorphism at position -786T>C (TT genotype) and Intron 4b/a (bb genotype) presented a better reduction of total cholesterol levels (-786T>C: before = 213 ± 12.1, after = 159.8 ± 14.4, Δ = -24.9% and Intron 4b/a: before = 211.8 ± 7.4, after = 180.12 ± 6.4 mg/dL, Δ = -15%), and LDL cholesterol (-786T>C: before = 146.1 ± 13.3, after = 82.8 ± 9.2, Δ = -43.3% and Intron 4b/a: before = 143.2 ± 8, after = 102.7 ± 5.8 mg/dL, Δ = -28.3%) in response to ET compared to those who carried the mutant allele. Superoxide dismutase activity was significantly increased in trained women whereas no changes were observed in malondialdehyde levels. Women without eNOS gene polymorphism at position -786T>C and Intron 4b/a showed a greater reduction of plasma cholesterol levels in response to ET. Furthermore, no genotype influence was observed on arterial blood pressure or oxidative stress status in this population.

eNOS genotype modifies the effect of leisure-time physical activity on serum triglyceride levels in a Japanese population

“…This suggests that the presence of the C allele enhances the beneficial effect of leisure-time physical activity on serum triglyceride levels. An intervention study showed that the presence of the C allele did not affect the beneficial effect of exercise in reducing serum triglyceride levels among postmenopausal women (n = 49) [19], but the sex difference of this effect was unclear in the current study.…”

[ARTIGO RETRATADO] Exercício e suas interações com vários aspectos da vida do homem e dos animais

“…A genetic study conducted by Esposti et al 25 endeavored to determine whether a single nucleotide polymorphism of the endothelial NO synthase (eNOS) gene at positions -786T>C, G894T (Glu298Asp) and at the variable number of tandem repeat Intron 4b/a would interfere with the cardiometabolic responses of trained postmenopausal women. Blood pressure was reduced after training, which was genotype-independent, but women without the studied eNOS gene polymorphism and Intron 4b/a (bb genotype) presented a better reduction of total cholesterol levels in response to training compared to those who carried the mutant allele.…”

Prevalência de dislipidemia em adultos de meia-idade com polimorfismo do gene NOS3 e baixa aptidão cardiorrespiratória

“…Além disso, esses autores observaram que essa variação polimórfica está relacionada a uma reduzida dissociação da enzima eNOS (35%) do complexo eNOS/ Caveolina-1, levando essa condição a uma redução da atividade enzimática e formação de nitro/nitrato. Estudo recente realizado no nosso laboratório demonstrou menor magnitude de redução do colesterol total e LDL-colesterol em resposta a oito semanas de treinamento físico aeróbio em mulheres com polimorfismo para as posições -786T>C e Glu298Asp do gene da eNOS em comparação àquelas que não apresentavam polimorfismo para essas posições, que foram estudadas de maneira isolada (16). Os possíveis mecanismos pelos quais a presença de polimorfismo para o gene da NOS3 estaria relacionada à maior prevalência de dislipidemia poderiam ser devido ao papel do NO sobre o metabolismo do colesterol.…”

“…Estudos prévios avaliaram a relação entre a presença de polimorfismos do gene da NOS3 e a prática de atividade física (11), o nível de aptidão cardiorrespiratória (12), bem como o efeito do treinamento físi-co (13)(14)(15)(16). No entanto, apenas um trabalho avaliou a influência do polimorfismo do gene da eNOS sobre parâmetros metabólicos em indivíduos treinados (16).…”

“…No entanto, apenas um trabalho avaliou a influência do polimorfismo do gene da eNOS sobre parâmetros metabólicos em indivíduos treinados (16). Além disso, até o presente momento nenhum estudo avaliou a interação entre os polimorfismos do gene da NOS3 (-786T>C, Glu298Asp e íntron 4b/a) e o nível de aptidão cardiorrespiratória.…”