Co-culture of chondrocytes and bone marrow mesenchymal stem cells in vitro enhances the expression of cartilaginous extracellular matrix components

Chang, Qing; Cui, Weiding; Fan, Weimin

doi:10.1590/s0100-879x2011007500026

Cited by 36 publications

(47 citation statements)

References 39 publications

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“…42 Importantly, MSCs have also been shown to stimulate cocultured cells to proliferate and synthesize extracellular matrix. [43][44][45] In fact, transplanted MSCs engrafted into the joint are activated and express Indian hedgehog and other genes. These genes in turn promote expression of collagen II and other chondrogenic genes by host cells.…”

Section: Discussionmentioning

confidence: 99%

Treatment of Knee Osteoarthritis With Allogeneic Bone Marrow Mesenchymal Stem Cells

et al. 2015

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Section: Discussionmentioning

confidence: 99%

Treatment of Knee Osteoarthritis With Allogeneic Bone Marrow Mesenchymal Stem Cells

et al. 2015

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“…These results show that the interactions between the cells in the micromass culture system can regulate the proliferation and differentiation of periosteal mesenchymal cells during chondrogenesis and that this effect is enhanced by TGF-β3. Qing et al 23 determined whether the co-culture of rabbit articular chondrocytes and BMSCs in vitro promotes the expression of cartilaginous extracellular matrix and, if so, what is the optimal ratio of the two cell types. Results demonstrate that the co-culture of rabbit chondrocytes and rabbit BMSCs at defined ratios can promote the expression of cartilaginous extracellular matrix.…”

Section: General Reviewmentioning

confidence: 99%

[ARTIGO RETRATADO] Revisão dos artigos sobre ortopedia recentemente publicados na imprensa científica brasileira

Rocha-e-Silva

Gomes

2012

Acta ortop. bras.

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This paper is a review of articles published in Brazilian scientific periodicals in recent years. Its main purpose is to bring to the attention of the readership of Acta Ortopedica Brasileira original contributions to the field published in non-specialized journals. We hope that this will serve as a general scientific update for readers. The review includes works published in six ISI indexed non-orthopedic journals, following a literature search conducted in fourteen such journals.

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“…In recent years, BMSC and chondrocyte coculture systems for cartilage construction have been investigated [Yang et al, 2009;Qing et al, 2011]. It was demonstrated that in coculture systems chondrocytes promoted the chondrogenesis of BMSCs and suppressed hypertrophy [Fischer et al, 2010], while BMSCs improved the chondrogenic and proliferative potential of engineered cartilage [Kang et al, 2012].…”

Section: Introductionmentioning

confidence: 99%

Different Ratios of Bone Marrow Mesenchymal Stem Cells and Chondrocytes Used in Tissue-Engineered Cartilage and Its Application for Human Ear-Shaped Substitutes in vitro

Kang¹,

Liu²,

Li³

et al. 2013

Cells Tissues Organs

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The application of chondrocyte-based cartilage tissue engineering is limited because of the lack of autologous cartilage sources and chondrocyte dedifferentiation after in vitro expansion. Coculture of bone marrow mesenchymal stem cells (BMSCs) and chondrocytes has been a promising strategy for cartilage engineering as chondrocytes can provide a chondrogenic environment for BMSCs. However, there are no systematic comparison studies for engineered cartilage constructed using different mixing ratios of BMSCs and chondrocytes, and the most effective mixing ratio with the lowest number of chondrocytes is unknown. Here, we set a gradient of mixing ratios of BMSCs to chondrocytes for an in vitro coculture system and compared the shape retention and quality of the engineered cartilage using macroscopic and histological assays, glycosaminoglycan content assessment and immunohistochemical staining of type II collagen, biomechanical evaluation and hypertrophy-related gene expression analysis. The results showed that at least 30% chondrocytes were required to generate cartilage tissue with satisfactory shape and quality. Therefore, we preliminarily assessed the feasibility of engineering a human ear-shaped substitute using a coculture system with a 7:3 ratio of BMSCs to chondrocytes. After 8 weeks of in vitro culture, the precise architecture of the human ear-shaped construct was well maintained with the typical cartilaginous composition confirmed by histological assays.

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Co-culture of chondrocytes and bone marrow mesenchymal stem cells in vitro enhances the expression of cartilaginous extracellular matrix components

Cited by 36 publications

References 39 publications

Treatment of Knee Osteoarthritis With Allogeneic Bone Marrow Mesenchymal Stem Cells

Treatment of Knee Osteoarthritis With Allogeneic Bone Marrow Mesenchymal Stem Cells

[ARTIGO RETRATADO] Revisão dos artigos sobre ortopedia recentemente publicados na imprensa científica brasileira

Different Ratios of Bone Marrow Mesenchymal Stem Cells and Chondrocytes Used in Tissue-Engineered Cartilage and Its Application for Human Ear-Shaped Substitutes in vitro

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