An improved strategy for evaluating the extent of chronic arterial baroreceptor denervation in conscious rats

Partial baroreceptor dysfunction and low plasma nitric oxide bioavailability as determinants of salt-sensitive hypertension: a reverse translational rat study

“…When TGSI was taken as an index of the extent of barodenervation (16) and the ΔpNOB was taken as an index of the change in sodium diet, the modeling of SSi (by multiple regression) indicated that ∼60% of the variation in SSi (r 2 =0.5730; ANOVA P=0.003) could be explained by the predictor variables TGSI (β=-4.357; P=0.003; power=92%), ΔpNOB (β=-0.2986; P=0.62; power=8%), and the TGSI*ΔpNOB interaction (β=1.6297; P=0.02; power=70%). In contrast, the reduction of this model (SSi) leaving only TGSI as the predictor variable, reduced the r 2 to 0.2368 (ANOVA P=0.02; power 74%).…”

“…In addition, the pdMAP was measured, and a validated individualized criterion of aortic barodenervation (TGSI) (16) was used in each rat. The general modeling of MAP data by mixed models showed that MAP differences between groups could be explained by the pdMAP, the effect of partial arterial barodenervation, the transition from a LNa to a HNa diet, and the interaction between partial arterial barodenervation and diet.…”

“…Using aseptic techniques and after administering ip ketamine (33.3 mg/kg; Anesket, PiSA) and xylazine (6.7 mg/kg; Rompun, Bayer, Mexico) anesthesia, atropinized (0.05 mg/kg ip ; Atropisa, PiSA) rats were subjected to either sham or real chronic bilateral aortic barodenervation using Krieger's technique (15) slightly modified by us (16). Sham or real barodenervations lasted 30-40 min.…”

“…The effectiveness of aortic barodenervation was evaluated in the conscious state using the transformed general sensitivity index (TGSI) previously described and validated by us (16). This index allows us to differentiate, on an individual basis, among rats with sham, aortic, or sino-aortic barodenervation: TGSI=[({[3−(ΔHR snp −ΔHR phe /ΔMAP snp −ΔMAP phe )] −0.4 }−1)/−0.04597].…”

“…This index allows us to differentiate, on an individual basis, among rats with sham, aortic, or sino-aortic barodenervation: TGSI=[({[3−(ΔHR snp −ΔHR phe /ΔMAP snp −ΔMAP phe )] −0.4 }−1)/−0.04597]. TGSI ranges previously established (16) were as follows: for sham barodenervation, 10.09 to 11.46; for aortic denervation, 8.40 to 9.94, and for sino-aortic denervation, 7.68 to 8.24.…”